Immunocytochemistry analysis of TLR9 in 4% paraformaldehyde-fixed HeLa cells using TLR9 polyclonal antibody (Product # PA5-85316) at a dilution of 1:100. Sample was then incubated with Hoechst secondary antibody.

Western blot was performed using Anti-TLR9 Polyclonal Antibody (Product # PA5-85316) and a 110 kDa band corresponding to CD289; Tlr9; toll like receptor 9; toll-like receptor 9; UNQ5798/PRO19605 was observed across in all cell lines. Whole cell extracts (40 µg lysate) of Daudi (Lane 1), HEL 92.1.7 (Lane 2), Raji (Lane 3), SH-SY5Y (Lane 4), IMR-32 (Lane 5), U-87 MG (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).Uncharacterized bands (*) were also observed in all the cell lines.