Fig 1: Activation of osteogenic and hypoxia signaling and calcification in the heart of CKD mice. (A) Scheme of the experimental protocol. (B) Body weight, (C) plasma urea, (D) plasma creatinine, (E) plasma phosphate levels in control (Ctrl) and CKD mice (n = 5/group). (F,G) Relative mRNA expressions of Runx2, Sox9, HIF-1a and HIF-1a normalized to HPRT from heart tissue derived from Ctrl and CKD mice (n = 5, measured in triplicates). (H) Bright-field and macroscopic fluorescence reflectance imaging of calcification and quantification in the heart of Ctrl and CKD mice (n = 5/group). Data are expressed as mean ± SD. Ordinary one-way ANOVA followed by Tukey's multiply comparison test was used to calculate p values. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.