SLK Antibody PA5-85695 from Thermo Fisher Scientific

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SLK Antibody

Immunofluorescence analysis of STE20-like serine/threonine-protein kinase was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SLK Polyclonal Antibody (Product # PA5-85695) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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Immunocytochemistry analysis of SLK in 4% paraformaldehyde-fixed H1299 cells using SLK polyclonal antibody (Product # PA5-85695) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
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Western blot was performed using Anti-SLK Polyclonal Antibody (Product # PA5-85695) and a 140 and180 kDa band corresponding to STE20-like serine/threonine-protein kinase was observed across all the cell lines5. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), HEK-293 (Lane 3), Caco-2 (Lane 4), NIH/3T3 (Lane 5), A-431 (Lane 6), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1500) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Multiple non specific bands (*) were also observed across samples tested.

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Thermo Fisher Scientific for
SLK Antibody

Thermo Fisher Scientific's SLK Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, and Western Blot. The SLK Antibody was generated using SLK as the antigen and it reacts with Homo Sapiens, and Human.

SLK Antibody

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SLK Antibody from Thermo Fisher Scientific

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SLK Antibody from Thermo Fisher Scientific

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