GAPDH Antibody PA5-85074 from Thermo Fisher Scientific

Product Specs
ItemGAPDH Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-85074
Quantity100 µL
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Concentration0.08 mg/mL
Conjugate/TagUnconjugated
FormatPBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
PurityAntigen affinity chromatography
ReactivityBacteria, Bovine, Canine, Chicken, Drosophila, Fish, Hamster, Human, Insect, Mouse, Nematode, Non-human primate, Porcine, Rabbit, Rat, Zebrafish
TargetGAPDH
IsotypeIgG
HostRabbit
ImmunogenRecombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary
NCBI Full Gene Nameglyceraldehyde-3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
NCBI Gene Aliasesgapd, HEL-S-162eP, bb02e05, Gapd-s, GAPDS, wu:fk58c09, fk58c09, wu:ft80f05, G3PD, wu:fb33a10, mg:bb02e05, fb71f08, KNC-NDS6, cb350, GAPD, gapdh-2, zgc:76908, cb609, BARS-38, Gapds, wu:fb71f08, Gapd, GAPDH-2, g3pdh, HSD-35, gapdh, gapds, GAPD2, HEL-S-278
ClonalityPolyclonal
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) BVDV utilizes PGC-1α downregulation to remodel the mitochondrial metabolic microenvironment, enhancing viral replication and impairing host immunityFrontiers in Veterinary ScienceMay 19, 2026Hongming Zhou, Yixing Zhao, Yuxin Kong, Jiying Yin, Ning He, Qi Wang, Jiayin Liu, Tongbo Zhou, Yu Han, Shouyi Dong, et al.in our laboratory. 2.2 Antibodies We used the following primary antibodies:Anti-GAPDH(Invitrogen, PA5-85074); Anti-BetaActin(AC026), Anti-Mfn2, Anti-NRF2(A21176), Anti-PGC1α(A20995), Anti-VDAC1(A15735), Anti-Drp1(A13699),

(2) Discovery of a pyrazolopyridine alkaloid that mitigates neuronal ER stress and age-related declineCommunications BiologyMay 8, 2026Salinee Jantrapirom, Apiwat Sangphukieo, Natsinee U-on, Pattaporn Poonsawas, Wasinee Wongkumool, Ranchana Yeewa, Chansunee Panto, Puttachat Poound, Ester Zito, Alice Marrazza, et al.(Abclonal, A23453, 1:1000), Caspase-3 (Cell Signaling, #9661, 1:1000), GAPDH (Thermo Fisher Scientific, PA5-85074, 1:5000), MAP2 (Merck, ZMS1013, 1:1000), phospho-eIF2α (Ser51) (Cell Signaling, #3398, 1:1000), phospho-PERK

(3) Helicobacter pylori CagA disrupts pancreatic epithelial barrier integrityFrontiers in Cell and Developmental BiologyNovember 18, 2025Anais Romero-Fabela, Angelica Silva-Olivares, Melisa Chacon, Erika P. Rendon-Huerta, Abigail BetanzosSanta Cruz Biotechnology; sc-25336, Dallas, TX, United States), and polyclonal anti-GAPDH (1:5,000; Invitrogen; PA5-85074, Carlsbad, CA, United States). HRP-conjugated secondary antibodies (anti-mouse or anti-rabbit; 1:3,000;

(4) High-Frequency Irreversible Electroporation Alters Proteomic Profiles and Tropism of Small Tumor-Derived Extracellular Vesicles to Promote Immune Cell InfiltrationCellsNovember 13, 2025Kelsey R. Murphy, Kenneth N. Aycock, Spencer Marsh, Liping Yang, Jonathan Hinckley, Aubrie Selmek, Robert Gourdie, Shay Bracha, Rafael V. Davalos, John H. Rossmeisl, et al.containing one of the following primary antibodies at 4 °C overnight: anti-GAPDH (Invitrogen, Cat #PA585074, 1:7500), anti-HSP70 (Invitrogen, Cat #MA3006, 1:1000), or anti-moesin (Invitrogen, Cat #PA5-70605,

(5) Harnessing macrophage-drug conjugates for allogeneic cell-based therapy of solid tumors via the TRAIN mechanismNature communicationsFebruary 4, 2025Taciak B, Bialasek M, Kubiak M, Marszalek I, Gorczak M, Osadchuk O, Kurpiel D, Strzemecki D, Barwik K, Skorzynski M, et al.8555), anti-ICAM1 (E3Q9N) XP (1:1000; Cell Signaling Technology, 67836), anti-GAPDH (1:5000, Invitrogen, PA5-85074), anti-lamin B1 (1:1000, Abcam, ab16048), anti-β-actin (1:10000; Proteintech, 66009-1) or anti-α-tubulin

(6) Targeting STAT3, FOXO3a, and Pim-1 kinase by FDA-approved tyrosine kinase inhibitor-Radotinib: An in silico and in vitro approachArchiv der PharmazieDecember 1, 2024Manoharan S, Santhakumar A, Perumal E.

(7) Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cellsBMC biologyAugust 26, 2024Luo X, Zhang D, Zheng J, Liu H, Sun L, Guo H, Wang L, Cui S.LPR6 (1:1000, M026228, Abmart), β-catenin (1:1000, 8480, Cell Signaling Technologies), GAPDH (1:2000, PA5-85074, Ambion) and α-tubulin antibody (1:1000, T40103, Abmart) at 4 ○C overnight. The membrane was then washed

(8) The Zn2+ transporter ZIP7 enhances endoplasmic-reticulum-associated protein degradation and prevents neurodegeneration in DrosophilaDevelopmental CellJuly 1, 2024Xiaoran Guo, Morgan Mutch, Alba Yurani Torres, Maddalena Nano, Nishi Rauth, Jacob Harwood, Drew McDonald, Zijing Chen, Craig Montell, Wei Dai, et al.proteins antibody, clone FK2 (Sigma-Aldrich, #04–263), 1:10.000 GAPDH antibody (Thermo Fisher Scientific, #PA5–85074), 1:500 anti-Actin (DSHB, #JLA20), 1:15.000 IRDye® 680LT Donkey anti-Mouse IgG Secondary Antibody (LI-COR Biosciences

(9) Effects of Green and Gold Kiwifruit Varieties on Antioxidant Neuroprotective Potential in Pigs as a Model for Human AdultsNutrientsApril 9, 2024Kanon AP, Giezenaar C, Roy NC, Jayawardana IA, Lomiwes D, Montoya CA, McNabb WC, Henare SJ.anti-SOD1 (Abcam, Cambridge, UK, ab13498), and rabbit anti-GAPDH (Thermo Scientific, Waltham, MA, USA, PA5-85074) diluted to 1:1000, 1:5000, 1:5000, and 1:10,000, respectively, in PBS-Tween. After washing with PBS-Tween,

(10) TRIM44 promotes autophagy through SQSTM1 oligomerization in the response to oxidative stress induced by Arsenic Trioxide in cancer cellsResearch SquareFebruary 21, 2024Nami McCarty, Yuqin Wang, Lin Lyu, Trung Vuantibody (R &D, MAB8525); anti-KEAP1 antibody (Origene, TA502059); anti-GAPDH antibody (Invitrogen, PA5–85074); anti-FLAG antibody (Invitrogen, PA1–984); anti-mTOR antibody (CST, 2972); anti-phospho-mTOR (Ser2448)

(11) Identification of Maleimide-Fused Carbazoles as Novel Noncanonical Bone Morphogenetic Protein SynergizersACS pharmacology & translational scienceAugust 11, 2023Riege D, Herschel S, Heintze L, Fenkl T, Wesseler F, Sievers S, Peifer C, Schade D.

(12) Tubulin tyrosination regulates synaptic function and is disrupted in Alzheimer's diseaseBrain : a journal of neurologyJuly 29, 2022Peris L, Parato J, Qu X, Soleilhac JM, Lanté F, Kumar A, Pero ME, Martínez-Hernández J, Corrao C, Falivelli G, et al.(anti-total tau (tau 46) (sc-32274) from Santa Cruz; anti-tau AT8 (MN1020) and anti-GAPDH (MA5-15738 and PA5-85074) from Invitrogen; anti-TTL (13618-1-AP) from Proteintech; anti-detyrosinated tubulin (MAB5566) from

(13) RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cellsAnnals of translational medicineMarch 1, 2022Luo X, Liu H, Guo H, Sun L, Gou K, Cui S.Cell Signaling Technologies), ERK1/2 (1:1,000; A4782; ABclonal), and internal control GAPDH (1:2,000, PA5-85074; Ambion) overnight at 4 ℃. The membrane was washed in TBST (0.1% Tween-20 in TBS) every 10 min and incubated

(14) Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoyAndrologiaFebruary 1, 2022Feng C, Wang Q, Deng L, Peng N, Yang M, Wang X.

(15) Crosstalk between acetylation and the tyrosination/detyrosination cycle of α-tubulin in Alzheimer's diseaseFrontiers in cell and developmental biologyJanuary 1, 2022Martínez-Hernández J, Parato J, Sharma A, Soleilhac JM, Qu X, Tein E, Sproul A, Andrieux A, Goldberg Y, Moutin MJ, et al.AT8 (MN1020 and 30505) from Invitrogen and Cell Signaling Technology, and anti-GAPDH (MA5-15738 and PA5-85074) from Invitrogen; anti acetylated tubulin antibody (T7451) from Sigma; anti α-tubulin antibody (11224-1-AP)

(16) TRIM44 enhances autophagy via SQSTM1 oligomerization in response to oxidative stressScientific reportsJanuary 1, 0001"Wang Y, Lyu L, Vu T, McCarty N."anti-LaminB1 antibody (R&D, MAB8525); antiKEAP1 antibody (Origene, TA502059); anti-GAPDH antibody (Invitrogen, PA5-85074); anti-FLAG antibody (Invitrogen, PA1-984); anti-mTOR antibody (CST, 2972); antiphospho-mTOR (Ser2448)

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GAPDH Antibody from Thermo Fisher Scientific

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GAPDH Antibody from Thermo Fisher Scientific

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