Immunocytochemical analysis of PRMT5 in HepG2 cells using a PRMT5 Monoclonal antibody (Product # MA5-32160) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western blot analysis was performed on nuclear enriched cell extract (30 µg lysate) of HeLa (Lane 1), T-47D (Lane 2), and Hep G2 (Lane 3). The blot was probed with Anti-PRMT5 Monoclonal Antibody (Product # MA5-32160, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 73 kDa band corresponding to PRMT5 was observed in all cell lines tested with a *non-specific band at ~160 kDa.