Knockdown of GPIP137 was achieved by transfecting A549 cells with specific siRNA (Silencer® select Product # s8377, s8378). (Fig1) Immunofluorescence analysis was performed on A549 cells (untransfected, panel a-d), transfected with GPIP137 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-GPIP137 Recombinant Rabbit Monoclonal Antibody (Product # 703658, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to GPIP137 green). The images were captured at 60X magnification. (Fig.2) shows fluorescent quantification analysis of GPIP137 siRNA immunofluorescence.