Immunofluorescence staining of CD97 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD97 Polyclonal Antibody (Product # PA5-80320, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.

Western Blot using CD97 Polyclonal Antibody (Product # PA5-80320) at 1:500 dilution. Lane A: HL-60 Membrane Lysate, Lane B: K562 Membrane Lysate. Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa.