Immunocytochemistry-Immunofluorescence analysis of NSE was performed in PC-12 cells using NSE Polyclonal Antibody (Product # PA5-88488) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of NSE in extracts of various cell lines. Samples were incubated with NSE Polyclonal antibody (Product # PA5-88488) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.