Western Blot was performed using Anti-EpoR Recombinant Rabbit Monoclonal Antibody (16) (Product # MA5-29248). In Fig.a, a 60 kDa band corresponding to Erythropoietin receptor was observed across cell lines tested except MCF-7 and SK-OV3 which is reported to be low. In Fig.b, a 95 kDa band corresponding to Erythropoietin receptor was upregulated during 5 days of HSC to erythroid differentiation and gradually downregulated in further differentiation process which is expected. In Fig.a, membrane enriched extracts (30 µg lysate) of K-562 (Lane 1), HEL 92.1.7 (Lane 2), MCF7 (Lane 3), SK-O-V3 (Lane 4) and in Fig.b, membrane enriched extracts (30 µg lysate) of Hematopoietic Stem Cells (Lane 1), HSC differentiated to Erythroid for 5 days (Lane 2), HSC differentiated to Erythroid for 10 days (Lane 3) and HSC differentiated to Erythroid for 15 days (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).