Immunofluorescence staining of HSP60 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with HSP60 Polyclonal Antibody (Product # PA5-80696, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Mitochondrion matrix.

Western Blot using HSP60 Polyclonal Antibody (Product # PA5-80696) at 1:500 dilution. Lane A: HepG2 whole cell lysate, Lane B: K562 whole cell lysate, Lane C: NIH-3T3 whole cell lysate, Lane D: A431 whole cell lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa. (We are unsure of the identity of these extra bands).