Western blot was performed using Anti-PAX2 Polyclonal Antibody (Product # PA5-81235) and a 40 to 45 kDa band corresponding to Paired box protein Pax-2 was observed across cell lines tested . Nuclear enriched extracts (50 µg lysate) of HEK-293 (Lane 1), SK-O-V3 (Lane 2), HaCaT (Lane 3), NIH:OVCAR-3 (Lane 4), MIA PaCa-2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).