Immunofluorescence staining of FOSL1 in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Fra1 Polyclonal Antibody (Product # PA5-81175) (1 µg/mL) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Western Blot using Fra1 Polyclonal Antibody (Product # PA5-81175) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 37 kDa.