Fig 1: Functional exploration of AKR1B10-mediated p53 regulation and pathway rescue. After transfection of siAKR1B10, AKR1B10 OE or siNC/EV control in SMMC7721 cells (A-B), qPCR was used to detect the expression of AKR1B10 and p53 mRNA. (C-E) Western blot analysis of AKR1B10 and p53 proteins under the same treatment conditions. After transfection of IER3-OE, RFX5-OE or co-transfection of AKR1B10-KD in SMMC7721 cells, (F-G) Western blot was used to detect the expression of p53 protein. (H) qPCR analysis of p53 mRNA under the same treatment conditions. Data are from three independent experiments and presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (One-way ANOVA)
Fig 2: Experimental verification of IER3-RFX5 functional interaction and AKR1B10 regulation. (A) Dual luciferase reporter gene transcription co-activation detection of IER3-RFX5 interaction. (B) Co-IP verification of the direct effect of IER3-RFX5 in SMMC7721 cells. SMMC7721 cells were transfected with siIER3, siRFX5, RFX5 OE + IER3 OE or siNC/EV control. (C-E) qPCR analysis of IER3, RFX5 and AKR1B10 mRNA expression in SMMC7721 cells. (F-I) Western blot analysis of IER3, RFX5 and AKR1B10 protein expression in SMMC7721 cells. Data are from three independent experiments and presented as mean ± SD. **p < 0.01, ***p < 0.001 (One-way ANOVA)
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