Anti-SNAP-tag Antibody (Polyclonal) P9310S from New England Biolabs

Product Specs
ItemAnti-SNAP-tag Antibody (Polyclonal)
CompanyNew England Biolabs
Price $299.00 Inquire
Catalog NumberP9310S
HostRabbit
TargetSNAP-Tag
Antibody TypePrimary Antibodies
Formataffinity purified using SNAP-BG resin
ApplicationsCellular Biology
Quantity100 µl
Conjugate/TagUnconjugated
ClonalityPolyclonal
TypeAntibody
Storage Temperature-20 C

Questions and Answers

Can this antibody be used for Western blotting applications?

Yes, the Anti-SNAP-tag Antibody has been utilized in Western blotting as indicated in multiple studies, including its application in detecting SNAP-tagged proteins in various experimental contexts, such as in the research by Kemter et al. (2021) and Tsuda et al. (2017).

What types of samples have been successfully analyzed using this antibody?

The Anti-SNAP-tag Antibody has been shown to work effectively in various biological samples, including primary and immortalized cells, as demonstrated in studies like those by Gandin et al. (2022) and Johnson et al. (2015), where it was used to detect SNAP-tagged proteins in cell lysates.

Is this antibody suitable for use in live-cell imaging experiments?

The Anti-SNAP-tag Antibody is primarily used in fixed-cell applications, as indicated by its use in studies that involve Western blotting and immunohistochemistry. However, it is important to note that the SNAP-tag system itself is compatible with live-cell imaging techniques, as seen in the work by Kemter et al. (2021), which utilized SNAP-tag for in vivo labeling.

What is the host species for this antibody, and how does that affect its use?

The Anti-SNAP-tag Antibody is produced in rabbits, which typically provides a strong immune response and high specificity. This can enhance the sensitivity and reliability of detection in various applications, including Western blotting and immunofluorescence, as noted in the literature.

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Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
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978-921-1350

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Website: http://www.neb.com

(1) Clathrin-independent endocytosis and retrograde transport in cancer cells tune immune synapse organization and CD8 T cell responseeLifeApril 22, 2026Shiqiang Xu, Alix Buridant, Thibault Hirsch, Céline Duhamel, Benjamin Ledoux, Massiullah Shafaq-Zadah, Estelle Dransart, Louise Thines, Ludger Johannes, Pierre Van Der Bruggen, et al.antibody,"Santa Cruz Biotechnology, sc-2354",AB_628490,WB,1:1000 Anti-SNAP-tag antibody,"New England Biolabs, P9310S",AB_10631145,WB,1:1000 Anti-Rab6A antibody,"Abcam, ab95954",AB_10679758,WB,1:1000 Anti-CD166 antibody,"Bio-Rad,

(2) The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stressLife Sci AllianceFebruary 5, 2026Tania Singh, Kyle A Jacobs, William J Polacheck, Matthew L Kutyssecondary antibodies (1:400) were from Invitrogen. DAPI nuclear stain was from Sigma-Aldrich. Anti-SNAP (P9310S, 1:1,000; WB) was from New England Biolabs. IRDye donkey anti-rabbit and anti-mouse IgG secondary antibodies

(3) Single-molecule dynamics of the TRiC chaperonin system in vivoNatureFebruary 4, 2026Rongqin Li, Niko Dalheimer, Martin B. D. Müller, F. Ulrich Hartl1/1,000 dilution), anti-GCN4 (SunTag) (Addgene, 218104-rAb, 1/1,000 dilution) and anti-SNAP-tag (NEB, P9310S, 1/1,000 dilution). Blots were then washed 3 times for 10 min with blocking buffer without low-fat milk

(4) Active fluctuations of cytoplasmic actomyosin networks facilitate dynein-driven transportiScienceDecember 19, 2025Takayuki Torisawa, Kei Saito, Ken’ya Furuta, Akatsuki Kimurapolystyrene beads (Invitrogen, F8811) were incubated with 0.6 μL of anti-SNAP antibody (New England Biolabs, P9310S) in a total of 50 μL BRB80 buffer (80 mM PIPES-KOH pH 6.8, 2 mM MgCl2, and 1 mM EGTA) for 20 minutes

(5) Proteomics- and BRET- screens identify SPRY2 as a Ras effector that impacts its membrane organizationiScienceDecember 19, 2025Karolina Pavic, Fiona Elizabeth Hood, Carla Jane Duval, Ganesh babu Manoharan, Christina Laurini, Farid Ahmad Siddiqui, Stephanie Puy Lam Mo, Ian Andrew Prior, Daniel Kwaku Abankwaanti-GAPDH (GAPDH71.1),Sigma,G8795; RRID: AB_1078991 Rabbit polyclonal anti-SNAP,New England Biolabs,P9310S; RRID: AB_10631145 Mouse monoclonal anti-B-Raf (F-7),Santa Cruz Biotechnology,sc-5284; RRID: AB_626760 Rabbit

(6) Rate-limiting enzymes in nucleotide metabolism synchronize nucleotide biosynthesis and chromatin formationMolecular CellDecember 18, 2025Shashank Srivastava, Daniela Samaniego-Castruita, Shubhi Srivastava, Sakshi Khurana, Jalees Rehman, Vipul Shukla, Issam Ben-Sahra, Daniel R. Foltz(Thermo Fisher Scientific R961–25), Streptavidin (Jackson ImmunoResearch 016 030–084), SNAP (New England Biolabs P9310S), NASP (Abcam ab181169), HSP90 (Cell Signaling Technologies 4874), Importin4 (Proteintech 11679–1-AP),

(7) Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fateNat CommunOctober 27, 2025Massiullah Shafaq-Zadah, Estelle Dransart, Ilyes Hamitouche, Christian Wunder, Valérie Chambon, Cesar A. Valades-Cruz, Ludovic Leconte, Nirod Kumar Sarangi, Jack Robinson, Siau-Kun Bai, et al.antibodies (kindly provided by Patrick Caswell), anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-CHC antibody (BD Bioscience, Ref. 610500), mouse anti-vinculin antibody (Sigma, Ref. V9131),

(8) Translation-independent association of mRNAs that encode protomers of the 5-HT2A-mGlu2 receptor complexJournal of Biological ChemistryJune 26, 2025Somdatta Saha, Javier Gonzalez-Maesocatalog no. 2278), anti-HA (Cell Signaling technology, catalog no. 3724), anti-SNAP (NEB, catalog no. P9310S), anti-RPS24 (Abcam, Catalog no. ab196652), or anti-IgG (RIP-Assay Kit, see above) antibodies. This

(9) Endogenous SNAP-Tagging of Munc13‑1 for Monitoring Synapse NanoarchitectureJACS AuJune 23, 2025Kowald M, Bachollet SPJT, Benseler F, Steinecker M, Boll M, Kaushik S, Soykan T, Sun S, Birke R, Ilic D, et al.Rabbit anti Munc13–1 (Synaptic Systems 126 103, diluted 1:1000), Rabbit anti SNAP (New England Biolabs, P9310S, diluted 1:500), Mouse anti synapsin 1 (Synaptic Systems 106 011, diluted 1:1000), Mouse anti Syntaxin

(10) Berberine is a Novel Mitochondrial Calcium Uniporter Inhibitor that Disrupts MCU-EMRE AssemblyAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2025Zhao H, Chen S, Cao N, Wu W, Liu G, Gao J, Chen J, Li T, Lu D, Zeng L, et al.Cell Signaling Technology, Cat# 14997 (1:1000); anti‐SNAP, rabbit polyclonal, New England Biolabs, Cat# P9310S (1:1000). Immunoprecipitation HEK293T cells were transfected with the indicated plasmids using Lipofectamine 2000 transfection reagent

(11) Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell divisionNat CommunApril 26, 2025Hien Bui, Simon Andersson, Agustin Sola-Carvajal, Tommaso De Marchi, Eliisa Vähäkangas, Minna Holopainen, Andrew H. House, Bohdana M. Rovenko, Johanna I. Englund, Maria Kasper, et al.(DM1A) antibody (Cell Signaling Technology, 3873, 1:1000); anti-SNAP-tag antibody (New England BioLabs, P9310S; 1:1,000); anti-beta Actin antibody (Abcam, ab8227, 1:2,000); Anti-UQCRFS1 [EPR16288] (Abcam, ab191078,

(12) Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cellsElifeApril 24, 2025Affiong Ika Oqua, Kin Chao, Liliane El Eid, Lisa Casteller, Billy P Baxter, Alba Miguéns-Gómez, Sebastian Barg, Ben Jones, Jorge Bernardino de la Serna, Sarah L Rouse, et al.and corresponding SNAP/FLAG-hGLP-1R levels detected by Western blotting with an anti-SNAP antibody (P9310S, New England Biolabs, RRID:AB_10631145). Data was represented as amount of bound Photo-Click cholesterol

(13) An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferationMolecular CellApril 17, 2025Jong-Sun Lee, Tu Dan, He Zhang, Yujing Cheng, Frederick Rehfeld, James Brugarolas, Joshua T. MendellRRID:AB_2182395 Anti-RPL23A,Proteintech,Cat#16386-1-AP; RRID:AB_2182395 Anti-SNAP-Tag,New England Biolabs,Cat#P9310S; RRID:AB_10631145 Anti-NMD3,Proteintech,Cat#16060-1-AP; RRID:AB_2282830 Anti-NHP2L1 (15.5K),Bethyl Laboratories

(14) Sub-organellar mitochondrial hydrogen peroxide observed using a SNAP tag targeted coumarin-based fluorescent reporter☆Redox BiologyMarch 1, 2025Ross Eaglesfield, Erika Fernandez-Vizarra, Erik Lacko, Stuart T. Caldwell, Nikki L. Sloan, Daniel Siciarz, Richard C. Hartley, Kostas Tokatlidisblocking, cells were incubated overnight at 4 °C with 1:500 dilution of SNAP primary antibody (NEB #P9310S) in 2 % w/v BSA in PBS. The next day cells were washed 3x in PBS at room temperature for 15 min and

(15) The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signalingNat CommunFebruary 4, 2025Naoyuki Kondo, Yuko Mimori-Kiyosue, Keizo Tokuhiro, Giuseppe Pezzotti, Tatsuo Kinashivector (N9183S), CLIP-Cell Starter Kit (E9200), CLIP-Cell TMR-Star (S9219S), and anti-SNAP-tag antibody (P9310S) were from New England Biolabs. Anti-phospho LC3 antibody (AP4013), and anti-CD18 antibody (BS6922)

(16) Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purificationJournal of Extracellular VesiclesOctober 14, 2024Madhusudhan Reddy Bobbili, André Görgens, Yan Yan, Stefan Vogt, Dhanu Gupta, Giulia Corso, Samir Barbaria, Carolina Patrioli, Sylvia Weilner, Marianne Pultar, et al.TA504796, 1:1000; Calnexin, abcam, ab22595; HA‐tag, Cell Signalling, 3724, 1:1000; SNAP/CLIP‐tag, NEB, P9310S, 1:1000; FLAG‐tag, Sigma, F3165, 1:5000). The membrane was washed with PBS supplemented with 0.1% Tween‐20

(17) RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursorsJ Cell BiolJune 3, 2024Dan Dou, Jayne Aiken, Erika L.F. HolzbaurGFP-1020, RRID:AB_10000240)","WB @ 1:5,000" "Anti-SNAP-tag, rabbit polyclonal","New England BioLabs (Cat# P9310S, RRID:AB_10631145)","WB @ 1:1,000" Secondary antibodies,Secondary antibodies,Secondary antibodies "Anti-rabbit

(18) Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologiesNature communicationsApril 17, 2024Zhu Y, Akkaya KC, Ruta J, Yokoyama N, Wang C, Ruwolt M, Lima DB, Lehmann M, Liu F.antibodies were used in this study: anti-HA (1:1000, mouse, Abcam, ab18181); anti-SNAP (1:1000, rabbit, NEB, P9310S); anti-MRPS18B, anti-TIMM44 and TOMM70 (1:500, 1:1000, 1:500, rabbit, ProteinTech, 16139-1-AP, 13859-1-AP,

(19) Molecular mechanism of dynein-dynactin complex assembly by LIS1ScienceMarch 29, 2024Kashish Singh, Clinton K. Lau, Giulia Manigrasso, José B. Gama, Reto Gassmann, Andrew P. Cartermonoclonal anti-GAPDH (Cat no. ab8245, Abcam, 1:1000 dilution), Rabbit polyclonal anti-SNAP-tag (Cat no. P9310S, New England Biolabs, 1:1000 dilution). Secondary antibodies used were: Goat anti-mouse IgG HRP (Cat

(20) Cardiac contraction and relaxation are regulated by distinct subcellular cAMP poolsNature chemical biologyJanuary 1, 2024Lin TY, Mai QN, Zhang H, Wilson E, Chien HC, Yee SW, Giacomini KM, Olgin JE, Irannejad R.from Abcam. Goat anti-β1AR antibody (EB07133) is from Everest Biotech. Rabbit anti-SNAP tag antibody (P9310S) is from New England Biolabs. Mouse anti-GM130 (610822) is from BD Biosciences. Sheep anti-TGN38 (AHP499G)

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Anti-SNAP-tag Antibody (Polyclonal)

Anti-SNAP-tag Antibody (Polyclonal) from New England Biolabs

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Anti-SNAP-tag Antibody (Polyclonal) from New England Biolabs

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