Knockdown of TYRO3 was achieved by transfecting HCT 116 cells with TYRO3 specific siRNA (Silencer® select Product # s14544, s14546). Western blot analysis (Fig a) was performed using membrane enriched extracts from the HCT 116 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-TYRO3 Recombinant Rabbit Monoclonal Antibody (Product # 703554, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to TYRO3.