Western blot analysis of CD3G on different lysates with Rabbit anti-CD3G antibody (ET7107-55) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MOLT-4 cell lysate Lane 3: Mouse thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 17/20 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-55) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.