Western blot analysis of Adiponectin in mouse tissue extract using 50 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with Adiponectin polyclonal antibody (Product # PA5-78288) using a dilution of 1:500.

Western Blot using Adiponectin Polyclonal Antibody (Product # PA5-78288). Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin Polyclonal Antibody (Product # PA5-78288) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 µg/mL gentamicin, 1 nM L-glutamin, 500 µM IBMX, 1 µM dexamethasone, 2 µM rosiglitazone and 1 µg/mL insulin.).