Western blot was performed using Anti-CDC37 Polyclonal Antibody (Product # PA5-77802) and a ~50 kDa band corresponding to CDC37 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane2), Jurkat (Lane 3), HEL 92.1.7 (Lane 4), HEK-293 (Lane 5) and NIH/3T3 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).