Fig 1: Human tRNA ?54 synthase activity is present in the cytoplasm. 2D-TLC of RNase T2 (A) and 1D-TLC (in solvent I) of nuclease P1 (B) digests of HeLa nuclear (NE) and cytoplasmic (CE) extract-treated [a-32P]UTP-labeled H. volcanii tRNATrp (Hv Trp). Relevant modified residues are labeled. “p” before and after a nucleoside letter in (A,B) indicates the 5' and 3' phosphate of that nucleoside. (C) Immunoblots of nuclear and cytoplasmic extracts using anti-Pus10 (HPA049582, Sigma) antibody. Purity of extracts was determined by using anti-lamin A (ab26300, Abcam) and anti-tubulin (ab6046, Abcam) antibodies as nuclear and cytoplasmic markers, respectively.
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