Use of Anti-Tubulin Antibody for ICC

Molecular Biosciences
University of Texas Austin
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

anti-beta tubulin

Catalog Number:

ab6046

Our lab is interested in the processes that govern T cell activation and focuses on identifying crucial events that govern this process. Because tubulin is known to localize to the immunological synapse during T cell activation, we used this antibody as an indicator of proper T cell activation.

Experimental Design and Results Summary

Applications

Immunofluorescence, Immunocytochemistry

Sample

Jurkat T cells

Primary Incubation

1:200, 4 C, overnight

Blocking Agent

Serum

Secondary Incubation

1:200, anti-rabbit secondary, 1 hr, room temperature

Tertiary Incubation

None

Detection

LEICA DMI4000B inverted microscope

Results Summary

This antibody works very well in all of the contexts that we use it and provides a bright signal

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works great and is reliable

The Bad

I can't think of any right now

The Bottom Line

Great antibody! We buy it all the time

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