CFTR R Domain Antibody MAB1660 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCFTR R Domain Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $399.00Supplier Page View Company Product Page
Catalog NumberMAB1660
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry
Quantity50 ug (also 25 ug)
TargetCFTR
HostMouse
IsotypeIgG1
Conjugate/TagUnconjugated
Clone13-1
ReactivityAmphibian,Hamster,Human,Mouse,Ovine,Rat,Frog - Xenopus (African Clawed Frog)
ClonalityMonoclonal
FormatSupplied as a solution in PBS containing BSA. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Immunogenß-galactosidase-coupled CFTR, Cys590-Lys830 (predicted), Accession # P13569
ConcentrationLYOPH
PurityProtein A or G purified from hybridoma culture supernatant
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening
Antibody TypePrimary Antibodies
NCBI Full Gene NameCF transmembrane conductance regulator
NCBI Gene AliasesTNR-CFTR, ABC35, CFTR/MRP, CF, dJ760C5.1, ABCC7, MRP7

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human CFTR. Specifically recognizes in vitro synthesized CFTR, recombinant CFTR protein, and non-recombinant CFTR protein (1, 2).

Sizes Available: 50 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the CFTR protein in human nasal epithelial organoids?

Yes, the CFTR R Domain Antibody has been utilized in human nasal epithelial organoid models to assess CFTR expression and function. In a study, the antibody was used for immunofluorescence staining to evaluate CFTR activity, demonstrating its applicability in this context ().

Is the CFTR R Domain Antibody effective for immunohistochemical studies in the developing human brain?

The CFTR R Domain Antibody has been shown to be effective in immunohistochemical studies of the developing human brain, where it was used to compare CFTR expression between normal and mutated forms in fetal brain samples ().

How has this antibody been applied in research related to cystic fibrosis?

The CFTR R Domain Antibody has been employed in various studies investigating cystic fibrosis, including its role in the trafficking and function of the F508del-CFTR mutant. It has been used to detect CFTR protein levels in cell lines and tissues, contributing to the understanding of therapeutic strategies for cystic fibrosis (, ).

What are the optimal conditions for using this antibody in immunoprecipitation assays?

In immunoprecipitation assays, the CFTR R Domain Antibody has been used successfully at a dilution of 1:50 in cell lysates. It is important to follow appropriate protocols for cell lysis and antibody incubation to ensure effective binding and detection of the CFTR protein ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signalingbioRxivApril 30, 2026Hung Caohuy, Mungunsukh Ognoon, Tinghua Chen, Qingfeng Yang, Thalia Dib, Bette S. Pollard, Naheed Fatima, Thomas Flagg, Dharmendra K. Soni, Roopa Biswas, et al.(Thermo Fisher). All antibodies were purchased commercially. β-actin (Sigma, A5441); CFTR (R&D Systems, MAB1660); CFTR (SCBT, sc-376683); β2AR (Novus Biologicals, NBP261710); TRADD (SCBT, sc-46653); TNFR1 (Cell Signaling,

(2) Culture and Imaging of Human Nasal Epithelial OrganoidsJournal of visualized experiments : JoVEDecember 17, 2021Liu Z, Anderson JD, Natt J, Guimbellot JS.Extract,Lonza,CC-4098, Insulin,Sigma,I-9278, Hydrocortisone,Sigma,H-0888, Triiodothyronine,Sigma,T-6397, Transferrin,Sigma,T-0665, Ethanolamine,Sigma,E-0135, Epinephrine,Sigma,E-4250, O-Phosphorylethanolamine,Sigma,P-0503, Retinoic Acid,Sigma,R-2625, Primary antibodies,,, Human CFTR antibody,R&D Systems,MAB1660,Dilution: 100x ZO-1 antibody,Thermo Fisher,MA3–39100-A647,Dilution: 1000x Anti-MUC5B antibody,Sigma,HPA008246,Dilution:

(3) Phosphorylation of the Chaperone-Like HspB5 Rescues Trafficking and Function of F508del-CFTRInternational Journal of Molecular SciencesJuly 8, 2020Fanny Degrugillier, Abdel Aissat, Virginie Prulière-Escabasse, Lucie Bizard, Benjamin Simonneau, Xavier Decrouy, Chong Jiang, Daniela Rotin, Pascale Fanen, Stéphanie Simonwashed twice with ice-cold PBS, incubated with primary monoclonal mouse antibody (Ab) against CFTR (clone 13.1 MAB1660, R&D Systems, Minneapolis, MN, USA) and polyclonal rabbit Ab against HspB5 (8851, Cell Signaling, Danvers,

(4) Human Nasal Epithelial Organoids for Therapeutic Development in Cystic FibrosisGenesMay 29, 2020Liu Z, Anderson JD, Deng L, Mackay S, Bailey J, Kersh L, Rowe SM, Guimbellot JS.antibodies at 4 °C for 2 days as follows: Anti-human CFTR (R&D Systems, Inc., Minneapolis, MN, R domain, MAB1660; 1:100), anti-human ZO-1 (Zona occludens 1; Thermo Scientific, Waltham, MA, USA, MA3-39100-A647; 1:1000),

(5) Changes in the R-region interactions depend on phosphorylation and contribute to PKA and PKC regulation of the cystic fibrosis transmembrane conductance regulator chloride channelFASEB bioAdvancesJanuary 1, 2020Poroca DR, Amer N, Li A, Hanrahan JW, Chappe VM.METHODS 2.1 Chemicals MM13‐4 and M3A7 mouse monoclonal anti‐CFTR antibodies were from Millipore‐Sigma. MAB1660 mouse monoclonal anti‐CFTR R‐region antibody was from R&D Systems. D6W6L rabbit monoclonal anti‐R‐region

(6) TALEN-Mediated Gene Targeting for Cystic Fibrosis-Gene TherapyGenesJanuary 11, 2019Xia E, Zhang Y, Cao H, Li J, Duan R, Hu J.Triton-100 in PBS). Cells were incubated with mouse monoclonal antibodies against the human CFTR R-domain MAB1660 and C-terminus MAB25031 (R&D Systems, Minneapolis, MN, USA) at 1:500 dilution overnight at 4 °C, washed

(7) Overcoming the Undesirable CRISPR-Cas9 Expression in Gene CorrectionMolecular therapy. Nucleic acidsDecember 7, 2018Xia E, Duan R, Shi F, Seigel KE, Grasemann H, Hu J.were separated on a 6.5% polyacrylamide gel, as described earlier. Anti-CFTR antibody (catalog no. MAB1660; R&D Systems, Minneapolis, MN, USA) was used at a concentration of 1:800. Five washes in 0.1% TBS-T

(8) Synergy of cAMP and calcium signaling pathways in CFTR regulationProceedings of the National Academy of Sciences of the United States of AmericaMarch 14, 2017Bozoky Z, Ahmadi S, Milman T, Kim TH, Du K, Di Paola M, Pasyk S, Pekhletski R, Keller JP, Bear CE, et al.

(9) Localization of cystic fibrosis transmembrane conductance regulator signaling complexes in human salivary gland striated duct cellsEuropean journal of oral sciencesJune 1, 2015Zinn VZ, Khatri A, Mednieks MI, Hand AR.LM Dilution – EM Chemicon AB3555 1:40 – 1:75 1:5 – 1:20 Mouse monoclonal anti-CFTR R&D Systems MAB1660 --- 1:10 – 1:25 Mouse monoclonal anti-ezrin Sigma-Aldrich E8897 --- 1:20 Abcam ab41672 1:150

(10) Mutating the Conserved Q-loop Glutamine 1291 Selectively Disrupts Adenylate Kinase-dependent Channel Gating of the ATP-binding Cassette (ABC) Adenylate Kinase Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and Reduces Channel Function in Primary Human Airway EpitheliaThe Journal of biological chemistryMay 29, 2015Dong Q, Ernst SE, Ostedgaard LS, Shah VS, Ver Heul AR, Welsh MJ, Randak CO.

(11) Cystic fibrosis transmembrane conductance regulator protein (CFTR) expression in the developing human brain: comparative immunohistochemical study between patients with normal and mutated CFTRThe journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyNovember 1, 2014Marcorelles P, Friocourt G, Uguen A, Ledé F, Férec C, Laquerrière A.carefully checked using different anti-CFTR antibodies (MAB3480 from Chemicon, Temecula, CA; MAB25031 and MAB1660 from R&D Systems, Abington, UK) and by testing them in parallel on lung, kidney and vas deferens sections

(12) A novel chimeric adenoassociated virus 2/human bocavirus 1 parvovirus vector efficiently transduces human airway epitheliaMolecular therapy : the journal of the American Society of Gene TherapyDecember 1, 2013Yan Z, Keiser NW, Song Y, Deng X, Cheng F, Qiu J, Engelhardt JF.

(13) Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosisPloS oneJanuary 1, 2011Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verzè G, Assael BM, et al.

(14) Functional Cystic Fibrosis Transmembrane Conductance Regulator Expression in Cystic Fibrosis Airway Epithelial Cells by AAV6.2-Mediated SegmentalTrans-SplicingHuman Gene TherapyMarch 5, 2009Yuhu Song, Howard H. Lou, Julie L. Boyer, Maria P. Limberis, Luk H. Vandenberghe, Neil R. Hackett, Philip L. Leopold, James M. Wilson, Ronald G. Crystalinstructions. The protein lysate was immunoprecipitated with 1.6 mg of anti-CFTR monoclonal antibody (clone MAB1660; R&D Systems, Minneapolis, MN), followed by immunoblotting with anti-CFTR antibody (kindly provided

(15) Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epitheliumThe Journal of clinical investigationFebruary 1, 2009Wong AP, Keating A, Lu WY, Duchesneau P, Wang X, Sacher A, Hu J, Waddell TK.type IV, vimentin, CD90, CD105, CD106, and CD73-PE (all from BD); collagen type I (Rockland); CFTR (MAB1660; R&D Systems); ENaC (AB3532P; Chemicon); acetylated α-tubulin IV (ab24610; Abcam); and AQP5 (178615;

(16) Molecular and Functional Characterization of CBAVD-Causing Mutations Located in CFTR Nucleotide-Binding DomainsCellular Physiology and BiochemistryJune 9, 2008Ana Grangeia, René Barro-Soria, Filipa Carvalho, Ana M Damas, Ana Colette Maurício, Karl Kunzelmann, Alberto Barros, Mário Sousaprotein in the cell surface and in the cytoplasm. CFTR was detected by a monoclonal mouse antibody MAB1660 (dilution 1:1000 in PBS, 1% BSA, 0.2% v/v Triton X-100; R&D Systems, Minneapolis, MN, USA), which recognizes

(17) Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airwaysAmerican journal of respiratory cell and molecular biologyJuly 1, 2006Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, et al.

(18) Chloride channels in the small intestinal cell line IEC-18Journal of cellular physiologyJanuary 1, 2005Basavappa S, Vulapalli SR, Zhang H, Yule D, Coon S, Sundaram U.

(19) Protection of Cftr knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epitheliaProceedings of the National Academy of Sciences of the United States of AmericaDecember 23, 2003Koehler DR, Sajjan U, Chow YH, Martin B, Kent G, Tanswell AK, McKerlie C, Forstner JF, Hu J.

(20) Mitochondria-rich cells as experimental model in studies of epithelial chloride channelsBiochimica et biophysica actaNovember 13, 2002Willumsen NJ, Amstrup J, Møbjerg N, Jespersen A, Kristensen P, Larsen EH.

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