Fig 2: a Radiation-induced changes in KGF expression in normal (KTC = primary keratinocytes, HaCat = immortalized keratinocytes, DF-19 = primary fibroblasts) and tumour cells (BW-225, ZMK-1, GR-145, OH-65). Error bars represent standard deviations. Measurements were performed in duplicate and experiments were repeated two times. b Radiation-induced changes in KGF receptor expression in keratinocytes (KTC) and HaCat cells. Error bars represent standard deviations. Measurements were performed in duplicate, and experiments were repeated two times

Fig 3: a No stimulated proliferation by recombinant KGF added to tumour cell cultures for 72 h. Open symbols represent cells plated in KGF-free medium; closed symbols represent cells plated in medium containing 0.2 μg/ml recombinant KGF. Data were recorded over 72 h, error bars represent ±1 standard deviation. Measurements were performed in triplicate and experiments were repeated at least three times. b No effect of recombinant KGF added to tumour cell cultures for 96 h. Closed bars represent cells plated in KGF-free medium; horizontal stripes represent cells plated in medium containing 0.2 μg/ml recombinant KGF. Data were recorded over 96 h, error bars represent ±1 standard deviation. Measurements were performed in triplicate, and experiments were repeated at least three times

Fig 4: a Example of KGF expression in normal (keratinocytes, fibroblasts DF-19) and tumour (ZMK-1, GR-145, BW-225, OH-65) cells. b KGF receptor expression in keratinocytes and ZMK-1 tumour cells

Fig 5: a No effect of the neutralizing anti-KGF antibody on the proliferation of fibroblasts (DF-19) or epithelial tumour cells (BW-225, ZMK-1, GR-145, OH-65) was found. Data were recorded for non-irradiated cells, 96 h after plating. Closed bars represent cells plated in KGF-free medium; dashed bars represent cells plated in medium containing 0.2 μg/ml recombinant KGF. Error bars represent ±1 standard deviation. Measurements were performed in triplicate and experiments were repeated at least three times. b No effect of the neutralizing KGF antibody on the proliferation of fibroblasts (DF-19) or epithelial tumour cells (BW-225, ZMK-1, GR-145, OH-65) was observed. Data were recorded for cells irradiated with 1 Gy, 96 h after plating. Closed bars represent cells plated in KGF-free medium; dashed bars represent cells plated in medium containing 0.2 μg/ml recombinant KGF. Error bars represent ±1 standard deviation. Measurements were performed in triplicate and experiments were repeated at least three times. c No effect of the neutralizing KGF antibody on the proliferation of fibroblasts (DF-19) or epithelial tumour cells (BW-225, ZMK-1, GR-145, OH-65) was found. Data were recorded for cells irradiated with 4 Gy, 96 h after plating. Closed bars represent cells plated in KGF-free medium; dashed bars represent cells plated in medium containing 0.2 μg/ml recombinant KGF. Error bars represent ±1 standard deviation. Measurements were performed in triplicate and experiments were repeated at least three times