Mouse SPARC Antibody AF942 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse SPARC Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF942
NCBI Gene AliasesON, BM-40
ApplicationsWestern Blo,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
TargetSPARC
HostGoat
ReactivityHuman,Mouse,Rat,Transgenic Mouse
NCBI Full Gene Namesecreted acidic cysteine rich glycoprotein
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin, Ala18-Ile302, Accession # P07214
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) SPARC upregulation mediates podocyte injury in Alport syndrome miceBMC NephrologyApril 3, 2026Dongxuan Chi, Hongyuan Chu, Jing Nie, Yanqin Zhang, Fang Wang, Jie Dingrespectively, incubated with ADGRB1 (1:100, Abcam, ab135907), goat-anti-mouse SPARC (1:200, R&D systems, AF942), rabbit-anti-human SPARC (1:100, Abcam, ab203284), overnight at 4℃. After washing thrice with PBS,

(2) ECM-Induced IL-23 Drives Immune Suppression in Breast Cancer via Regulating PD-1 on TregsJournal of Experimental & Clinical Cancer ResearchSeptember 1, 2025Giovanna Talarico, Mara Lecchi, Anna Zanichelli, Paola Portararo, Laura Botti, Vera Cappelletti, Massimo Costanza, Annamaria Piva, Pietro Pratesi, Francesco Bertolini, et al.were incubated overnight at 4 °C with the following antibodies: goat anti-mouse SPARC IgG (catalog #AF942, R&D Systems; dilution 1:500) and monoclonal mouse anti-mouse/human vinculin (clone hVIN-1, catalog

(3) Dual lineage origins contribute to neocortical astrocyte diversityNature communicationsJuly 30, 2025Zhou J, Vitali I, Roig-Puiggros S, Javed A, Cantando I, Puglisi M, Bezzi P, Jabaudon D, Mayer C, Bocchi R.(Millipore, MAB1596, 1:600), goat anti-EGFR (R&D Systems, FAB9577B, 1:1000), goat anti-Sparc (R&D Systems, AF942; 1:200), goat anti-sparcl1(R&D Systems, AF2836, 1:1000), mouse anti-Gad2 (Merck, MAB5406; 1:500), guineapig

(4) Astrocyte modulation of synaptic plasticity mediated by activity-dependent Sonic hedgehog signalingJ NeurosciMarch 12, 2025Anh Duc Le, Marissa Fu, Ashley Carper, Elizabeth Zegarowicz, Riya Kumar, Gloria Zacharias, A. Denise R. Garciaresource,Source,Identifier Antibodies,Antibodies,Antibodies Polyclonal goat anti-SPARC,R&D Systems,AF942 Polyclonal goat anti-SPARC-like 1 (SPARCL1/Hevin),R&D Systems,AF2836 Polyclonal chicken anti-β-Gal,Abcam,AB9361 Polyclonal

(5) Astrocyte Modulation of Synaptic Plasticity Mediated by Activity-Dependent Sonic Hedgehog SignalingJ NeurosciFebruary 3, 2025Anh Duc Le, Marissa Fu, Ashley Carper, Elizabeth Zegarowicz, Riya Kumar, Gloria Zacharias, A. Denise R. Garciaresource,Source,Identifier Antibodies,Antibodies,Antibodies Polyclonal goat anti-SPARC,R&D Systems,AF942 Polyclonal goat anti-SPARC-like 1 (SPARCL1/Hevin),R&D Systems,AF2836 Polyclonal chicken anti-β-Gal,Abcam,AB9361 Polyclonal

(6) Astrocyte modulation of synaptic plasticity mediated by activity-dependent Sonic hedgehog signalingbioRxiv : the preprint server for biologyJune 13, 2024Le AD, Fu M, Kumar R, Zacharias G, Garcia ADR.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,, Polyclonal Goat anti SPARC,R&D Systems,AF942 Polyclonal Goat anti SPARC-like 1 (SPARCL1/ Hevin),R&D Systems,AF2836 Polyclonal Chicken anti β-Gal,Abcam,AB9361 Polyclonal

(7) Abnormal Morphology and Synaptogenic Signaling in Astrocytes Following Prenatal Opioid ExposureCellsMay 14, 2024Niebergall EB, Weekley D, Mazur A, Olszewski NA, DeSchepper KM, Radant N, Vijay AS, Risher WC.Minneapolis, MN, USA), mouse anti-hevin (1:2000; R&D Systems AF2836), mouse anti-SPARC (1:1000; R&D Systems AF942), goat anti-TSP1 (1:1000; R&D Systems AF3074), goat anti-TSP2 (1:1000; R&D Systems AF1635), or goat

(8) A branching model of lineage differentiation underpinning the neurogenic potential of enteric gliaNature communicationsSeptember 22, 2023Laddach A, Chng SH, Lasrado R, Progatzky F, Shapiro M, Erickson A, Sampedro Castaneda M, Artemov AV, Bon-Frauches AC, Amaniti EM, et al.

(9) Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial CellsCellsJanuary 16, 2023Song YS, Jamali N, Sorenson CM, Sheibani N.

(10) Altered excitatory and inhibitory neocortical circuitry leads to increased convulsive severity after pentylenetetrazol injection in an animal model of schizencephaly, but not of microgyriaEpilepsia openSeptember 1, 2022Dos Santos Heringer L, Rios Carvalho J, Teixeira Oliveira J, Texeira Silva B, de Souza Aguiar Dos Santos DM, Martinez Martinez Toledo AL, Borges Savoldi LM, Magalhães Portela D, Adriani Marques S, Campello Costa Lopes P, et al.antibodies GAP‐43 (Ab75810),Rabbit,1:100,Abcam Gephyrin (sc25311),Mouse,1:100,Santa Cruz SPARC (AF942),Mouse,1:100,R&D systems Secondary antibodies,Secondary antibodies,Secondary antibodies,Secondary antibodies Alexa

(11) Activity of acute pancreatitis is modified by secreted protein acidic and rich in cysteine ablationUnited European gastroenterology journalJuly 1, 2022Ammer-Herrmenau C, Wolf L, Nasrin SS, Ramu I, Roggiolani R, Goetze RG, Buchholz SM, Sendler M, Ellenrieder V, Neesse A.standard protocol as previously stated. 18 The following antibodies were used: SPARC (R&D Systems, AF942, 1:100, Research Resource Identifiers (RRID): Antibody (AB)_2286625), α‐SMA (Dako, Clone 1A4, 1:250,

(12) Effect of SPARC Suppression in Mice, Perfused Human Anterior Segments, and Trabecular Meshwork CellsInvestigative ophthalmology & visual scienceJune 1, 2022MacDonald WW, Swaminathan SS, Heo JY, Castillejos A, Hsueh J, Liu BJ, Jo D, Du A, Lee H, Kang MH, et al.Sigma-Aldrich), fibronectin (ab23750; Abcam, Cambridge, MA, USA), laminin (AB2034; EMD Millipore), and SPARC (AF942; R&D Systems, Minneapolis, MN, USA). Sections were washed with xylene and subsequently hydrated with

(13) Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse developmentCell reportsFebruary 22, 2022Xie Y, Kuan AT, Wang W, Herbert ZT, Mosto O, Olukoya O, Adam M, Vu S, Kim M, Tran D, et al.RRID:AB_2138836 Goat monoclonal anti-Il33,Novus Bio,AF3626; RRID:AB_884269 Goat monoclonal anti-Sparc,R&D Systems,AF942; RRID:AB_2286625 Rabbit monoclonal anti-Kir4.1,Millipore,AB5818; RRID:AB_92052 Rabbit monoclonal anti-Olig2,Millipore,AB9610;

(14) Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Leads to an Accelerated Ageing Phenotype Which Is Improved by Exercise Whereas SPARC Overexpression Mimics Exercise Effects in MiceMetabolitesJanuary 28, 2022Ghanemi A, Melouane A, Yoshioka M, St-Amand J.overnight with 1/250 dilution (in PierceTM Protein-Free (TBS) blocking buffer) of the primary antibody (AF942, R&D Systems, Inc., Minneapolis, MN, USA), and a 2 h incubation with 1/1000 dilution (in PierceTM Protein-Free

(15) USP22 Suppresses SPARC Expression in Acute Colitis and Inflammation-Associated Colorectal CancerCancersApril 10, 2021Kosinsky RL, Saul D, Ammer-Herrmenau C, Faubion WA, Neesse A, Johnsen SA.h and antibodies detecting CD45 (553089, BD Biosciences, Franklin Lakes, NJ, USA, 1:200) and SPARC (AF942, R&D Systems, Minneapolis, MN, USA, 1:200) were utilized. Biotinylated secondary antibodies (1:200;

(16) Tracing tumorigenesis in a solid tumor model at single-cell resolutionNature communicationsFebruary 20, 2020Praktiknjo SD, Obermayer B, Zhu Q, Fang L, Liu H, Quinn H, Stoeckius M, Kocks C, Birchmeier W, Rajewsky N.(Boster, PA1709), mouse-anti-Ptn (Santa Cruz Biotechnology, SC74443), goat-anti-Sparc (R&D Systems; AF942). Secondary antibodies were conjugated with Cy2, Cy3, or Cy5 fluorochromes (Jackson ImmunoResearch Laboratories).

(17) Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cellsPloS oneJanuary 1, 2020Falero-Perez J, Sheibani N, Sorenson CM.antibody (MS-421P clone A6.1; NeoMarker, Fremont, CA), anti-osteopontin (AF808; R&D Systems), anti-SPARC (AF942; R&D Systems) and anti-β-actin (MAB-515739; Thermofisher), anti-phospho JNK (Thr221;#AF-1205; R&D Systems),

(18) Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal DevelopmentFrontiers in neuroscienceJanuary 1, 2020Shoneye T, Orrego AT, Jarvis R, Men Y, Chiang MSR, Yang Y.against Ki-67 (1:100 rabbit anti-Ki-67, Pierce #PA5-19462), Sparc (1:500 goat anti-Sparc, R&D Systems #AF942), or Sox9 (1:100 goat anti-Sox9, R&D Systems #AF3075) were incubated overnight at 4°C in the appropriate

(19) Stromal Features of the Primary Tumor Are Not Prognostic in Genetically Engineered Mice of Pancreatic CancerCellsDecember 24, 2019Hasselluhn MC, Klein L, Patzak MS, Buchholz SM, Ströbel P, Ellenrieder V, Maisonneuve P, Neesse A.USA",RM-9106-S0,IHC/IF α-SMA,"Dako Denmark A/S; Glostrup, Denmark",M0851,IHC/IF SPARC,"R and D Systems, Inc.; Minneapolis, MN, USA",AF942,IHC Antibodies used for immunohistochemistry (IHC) and immunofluorescence (IF) staining. Abbreviations:

(20) Single-cell transcriptomic profiling of the aging mouse brainNature neuroscienceOctober 1, 2019Ximerakis M, Lipnick SL, Innes BT, Simmons SK, Adiconis X, Dionne D, Mayweather BA, Nguyen L, Niziolek Z, Ozek C, et al.antibodies in blocking solution (typically at 1:100 dilution): goat polyclonal anti-SPARC (R&D Systems #AF942), rabbit polyclonal anti-IBA1 (Wako #019-19741), goat polyclonal anti-IL33 (R&D Systems #AF3626), and

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Mouse SPARC Antibody

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Mouse SPARC Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Mouse SPARC Antibody from R&D Systems, a Bio-Techne Brand

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