EPCR Antibody AF2245 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEPCR Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF2245
NCBI Gene AliasesCCCA, EPCR, CCD41
ApplicationsBinding Assay,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Dot Blot,Neutralization,Immunocytochemistry,CyTOF-ready
ReactivityHuman,Mouse
TargetEPCR
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
Quantity100 ug (also 25 ug)
IsotypeIgG
NCBI Full Gene Nameprotein C receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human EPCR, Ser18-Ser210, Accession # Q9UNN8
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse EPCR is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Febrile temperature enhances Plasmodium falciparum cytoadhesion by disrupting the endothelial glycocalyxbioRxivSeptember 16, 2025Viola Introini, Rory Long, Olawunmi Rashidat Oyerinde, Silvia Sanz Sender, Frank Stein, Gyu Min Hwang, Borja Lopez Gutierrez, Karl Boynton Seydel, Gretchen Birbeck, Maria Bernabeuincubated for 30 minutes. The following antibodies were used: goat anti-human EPCR (5 μg/ml; R&D Systems, AF2245), followed by donkey anti-goat Alexa Fluor 647-conjugated secondary antibody (1:400; Invitrogen, A32849);

(2) Non-myogenic mesenchymal cells contribute to muscle degeneration in facioscapulohumeral muscular dystrophy patientsCell death & diseaseSeptember 16, 2022Di Pietro L, Giacalone F, Ragozzino E, Saccone V, Tiberio F, De Bardi M, Picozza M, Borsellino G, Lattanzi W, Guadagni E, et al.antibodies in PBS with 1% of BSA: anti-laminin (1:400, #L9393, Sigma-Aldrich), anti-CD201 (1:100, #AF2245, R&D systems, Minneapolis, MN, USA) anti-PDGFRα (1:500, #3174, Cell Signalling Technology, Danvers,

(3) EPCR-PAR1 biased signaling regulates perfusion recovery and neovascularization in peripheral ischemiaJCI insightJuly 22, 2022Bochenek ML, Gogiraju R, Großmann S, Krug J, Orth J, Reyda S, Georgiadis GS, Spronk HM, Konstantinides S, Münzel T, et al.label platelets, eNOS (D9A5L; Cell Signaling Technology; 32027; dilution, 1:100), EPCR (R&D Systems; AF2245; dilution, 1:100), fibrinogen (Abcam; clone EPR18145-84; ab189490; dilution, 1:100), HBA (LifeSpan BioSciences;

(4) Role of thrombomodulin expression on hematopoietic stem cellsJournal of thrombosis and haemostasis : JTHJanuary 1, 2020Basu S, Liang HPH, Hernandez I, Zogg M, Fields B, May J, Ogoti Y, Wyseure T, Mosnier LO, Burns RT, et al.primary antibodies (sheep anti-human Thbd: AF3947; goat anti-mouse Thbd: AF3894; goat anti-human EPCR: AF2245; R&D Systems, Minneapolis, MN; goat-anti mouse EPCR; kind gift from Dr. Charles Esmon, Oklahoma Medical

(5) Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruptionScientific reportsSeptember 11, 2019Avril M, Benjamin M, Dols MM, Smith JD.anti-human CX3CL1/Fractalkine (15 μg/ml, R&D Systems, AF365) or goat anti-human EPCR (30 μg/ml, R&D Systems, AF2245) followed by rabbit anti-goat Alexa488 coupled antibodies (A-21467, Molecular Probes, 1/400). Mouse

(6) Human platelets express endothelial protein C receptor, which can be utilized to enhance localization of factor VIIa activityJournal of thrombosis and haemostasis : JTHSeptember 1, 2018Fager AM, Machlus KR, Ezban M, Hoffman M.A/G beads: Thermo Scientific (Rockford, IL). Affi-Gel 10: Bio-Rad (Hercules, CA). Goat-anti-hEPCR (AF2245), Mouse-anti-hEPCR (MAB22451), and isotype controls: R&D Systems (Minneapolis, MN). Donkey-anti-sheep/goat

(7) An ADAM-10 dependent EPCR shedding links meningococcal interaction with endothelial cells to purpura fulminansPLoS pathogensApril 1, 2018Lécuyer H, Virion Z, Barnier JP, Matczak S, Bourdoulous S, Bianchini E, Saller F, Borgel D, Nassif X, Coureuil M.

(8) Infected erythrocytes expressing DC13 PfEMP1 differ from recombinant proteins in EPCR-binding functionProceedings of the National Academy of Sciences of the United States of AmericaJanuary 30, 2018Azasi Y, Lindergard G, Ghumra A, Mu J, Miller LH, Rowe JA.effect of antibodies, HBEC-5i was preincubated with 20 μg/mL goat polyclonal antibody to human EPCR (AF2245; R&D Systems), rat monoclonal antibody to human EPCR (clone RCR-252, HM2145; Hycult), or isotype control

(9) Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytesPloS oneJanuary 1, 2018Ecklu-Mensah G, Olsen RW, Bengtsson A, Ofori MF, Hviid L, Jensen ATR, Adams Y.

(10) Interaction between Endothelial Protein C Receptor and Intercellular Adhesion Molecule 1 to Mediate Binding of Plasmodium falciparum-Infected Erythrocytes to Endothelial CellsMBioJuly 12, 2016Avril M, Bernabeu M, Benjamin M, Brazier AJ, Smith JD.

(11) Plasma endothelial protein C receptor influences innate immune response in ovarian cancer by decreasing the population of natural killer and TH17 helper cellsInternational journal of oncologyOctober 1, 2013Azzazene D, Al Thawadi H, Al Farsi H, Besbes S, Geyl C, Mirshahi S, Pardo J, Faussat AM, Jeannette S, Therwath A, et al.patients. Materials and methods Reagents Reagents were obtained from following sources: primary antibody AF2245 against the EPCR (R&D Systems, Minneapolis, MN, USA); primary antibody ATAP2 against PAR-1 (Invitrogen,

(12) Severe malaria is associated with parasite binding to endothelial protein C receptorNatureJune 27, 2013Turner L, Lavstsen T, Berger SS, Wang CW, Petersen JE, Avril M, Brazier AJ, Freeth J, Jespersen JS, Nielsen MA, et al.

(13) An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasisJournal of proteomicsDecember 21, 2012Karhemo PR, Ravela S, Laakso M, Ritamo I, Tatti O, Mäkinen S, Goodison S, Stenman UH, Hölttä E, Hautaniemi S, et al.was also required. 2.5. Antibodies We used the following primary antibodies: anti-PROCR (anti-EPCR, AF2245, R&D Systems Inc. Minneapolis, USA), anti-CD109 (AF4385, R&D Systems Inc., Minneapolis, USA), anti-integrin-α6

(14) Soluble endothelial protein C receptor (sEPCR) is likely a biomarker of cancer-associated hypercoagulability in human hematologic malignanciesCancer medicineOctober 1, 2012Ducros E, Mirshahi SS, Faussat AM, Mirshahi P, Dimicoli S, Tang R, Pardo J, Ibrahim J, Marie JP, Therwath A, et al.treatment implications in patient care. Materials and Methods Antibodies and primers Primary antibody AF2245 against the EPCR (R&D Systems, Lille, France); biotinylated anti-goat IgG, streptavidin-fluorescein

(15) Endothelial protein C receptor expressed by ovarian cancer cells as a possible biomarker of cancer onsetInternational journal of oncologyAugust 1, 2012Ducros E, Mirshahi S, Azzazene D, Camilleri-Broët S, Mery E, Al Farsi H, Althawadi H, Besbess S, Chidiac J, Pujade-Lauraine E, et al.lines. Materials and methods Reagents Reagents were obtained from the following sources: primary antibody AF2245 against EPCR (R&D Systems, Minneapolis, MN, USA); primary antibody ATAP2 against PAR-1 (Invitrogen,

(16) Cellular regulation of blood coagulation: a model for venous stasisBloodDecember 23, 2010Campbell JE, Brummel-Ziedins KE, Butenas S, Mann KG.

(17) Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancerJournal of proteome researchOctober 1, 2009Gunawardana CG, Kuk C, Smith CR, Batruch I, Soosaipillai A, Diamandis EP.

(18) Analysis of Endothelial Protein C Receptor Functionality on Living Cells’TOBIOCJMay 13, 2009E Ducros, S Mirshahi, C Bermot, M Mirshahicells were thereafter incubated for 1 h at room temperature with 20 µg/ml of EPCR specific antibodies (AF2245, R&D Systems, Minneapolis, MN, USA), and then washed with PBS. The slides were then incubated successively

(19) Analysis of endothelial protein C receptor functionality on living cells'The Open Biochemistry JournalMay 13, 2009E Ducros, S Mirshahi, C Bermot, M Mirshahicells were thereafter incubated for 1 h at room temperature with 20 g/ml of EPCR specific antibodies (AF2245, R&D Systems, Minneapolis, MN, USA), and then washed with PBS. The slides were then incubated successively

(20) Aldosterone modifies hemostasis via upregulation of the protein-C receptor in human vascular endotheliumBiochemical and biophysical research communicationsAugust 22, 2008Ducros E, Berthaut A, Mirshahi SS, Faussat AM, Soria J, Agarwal MK, Mirshahi M.

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EPCR Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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EPCR Antibody from R&D Systems, a Bio-Techne Brand

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