DPPIV/CD26 Antibody AF954 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemDPPIV/CD26 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF954
NCBI Gene AliasesADCP2, ADABP, CD26, Cd26, Dpp-4, TP103, DPPIV, THAM
ApplicationsImmunodepletion,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetDPPIV/CD26
HostGoat
IsotypeIgG
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Rat,Transgenic Mouse
NCBI Full Gene Namedipeptidyl peptidase 4, dipeptidylpeptidase 4
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse DPPIV/CD26, Ser29-His760, Accession # P28843
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human DPPIV/CD26 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used for immunohistochemistry in mouse tissues?

Yes, the DPPIV/CD26 antibody has been shown to be effective for immunohistochemistry applications in mouse tissues, as evidenced by its use in various studies, including those investigating the cellular composition of mouse teeth and the role of DPP4 in scleroderma models.

What is the specificity of this antibody when detecting DPPIV/CD26?

The DPPIV/CD26 antibody specifically detects mouse DPPIV/CD26 with less than 20% cross-reactivity with recombinant human DPPIV/CD26, making it suitable for studies focused on mouse models.

Has this antibody been utilized in any studies related to obesity and insulin resistance?

Yes, the DPPIV/CD26 antibody was used in a study that demonstrated the role of hepatocyte-secreted DPP4 in promoting adipose inflammation and insulin resistance in obesity, highlighting its relevance in metabolic research.

What applications has this antibody been cited in within the scientific literature?

The DPPIV/CD26 antibody has been cited in applications such as Western blotting, flow cytometry, immunocytochemistry, and immunohistochemistry, indicating its versatility in various experimental setups.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunityCellJanuary 8, 2026Emily Scott-Solomon, Shlomi Brielle, Alexander O. Mann, Mark J. Khoury, Jingyu Peng, Liana Tellez, Mackenzie Harrigan, Myrto Ziogas, H. Amalia Pasolli, Monica Cassandras, et al.(1:300),MilliporeSigma,Catalog# C6198; RRID:AB_476856 Goat anti CD26 (1:100),R&D Systems,Catalog# AF954; RRID:AB_355739 Guinea pig anti Trp2 (1:1200),Lab generated,N/A Rat anti CD34 (1:50),Thermo Fisher Scientific

(2) Unique Role of Proximal Tubule Dipeptidyl Peptidase 4 on Blood Pressure, Renal Sodium Handling, and Na + /H + Exchanger Isoform 3 PhosphorylationActa Physiol (Oxf)December 1, 2025Flavia L. Martins, Joao Carlos Ribeiro‐Silva, Erika Fernandes de Jesus, Ravi Nistala, Adriana C. C. Girardimin. Sections were incubated overnight at 4°C with primary antibodies against DPP4 (1:100, catalog #AF954, R&D Systems) and SGLT2 (1:100, catalog #20802, Bicell) in blocking solution. Following three 5‐min

(3) 3D imaging and single-cell analysis reveal cellular heterogeneity of lymphatic valve endothelial cell typesiScienceNovember 21, 2025Emmanuelle Marchaud, Renaud Morin, Jason S. Iacovoni, Tangra Draia-Nicolau, Aurélie Gomes, Marine Norlund, Pascale Bernes-Lasserre, Jean-Michel Lagarde, Anne-Catherine Prats, Barbara Garmy-Susini, et al.polyclonal antibody conjugated with Alexa Fluor 647 (NB110-40760AF647, Novus Biologicals), goat anti-DPP4 (AF954, R&D system), mouse anti-human αSMA (clone 1A4, M0851, Dako), goat anti-LYVE1 (AF2125, R&D system).

(4) TGF-β-mediated epithelial-mesenchymal transition of keratinocytes promotes fibrosis in secondary lymphedemaJCI insightSeptember 9, 2025Park HJ, Shin J, Sarker A, Klang MG, Riedel E, Coriddi M, Dayan JH, Pal S, Mehrara BJ, Kataru RP.incubated with antibodies against fibronectin (ab2413; Abcam, Cambridge, UK), MMP9 (ab38898; Abcam), CD25 (AF954; R&D Systems, Inc., Minneapolis, MN, USA), vimentin (ab137321; Abcam), FSP1 (ABF32; Sigma-Aldrich),

(5) Identification of the velum interpositum as a meningeal-CNS route for myeloid cell trafficking into the brainNeuronAugust 6, 2025Lindsay A. Hohsfield, Sung Jin Kim, Rocio A. Barahona, Caden M. Henntingfield, Kimiya Mansour, Kristen D. Vallejo, Kate I. Tsourmas, Nellie E. Kwang, Yasamine Ghorbanian, Julio Alejandro Ayala Angulo, et al.L9393, Sigma), TIE2 (1:100, ab24859, Abcam), LYVE1 (1:200, ab14917, Abcam), DPP4 (1:200, R&D Systems, AF954), MMP2 (1:200, ab97779, Abcam), NeuN (1:1000; MAB377, EMD Millipore), RFP (1:200, 600–401-379, Rockland),

(6) CD206+ macrophages facilitate wound healing through interactions with Gpnmbhi fibroblastsEMBO ReportsJune 10, 2025Azusa Honda, Hiroyuki Koike, Teruyuki Dohi, Eri Toyohara, Sumio Hayakawa, Kazuyuki Tobe, Ichiro Manabe, Rei Ogawa, Yumiko OishiCA, USA; 1:200), anti-engrailed-1 (PA5-14149, Thermo Fisher Scientific, CA, USA; 1:200), anti-CD26 (AF954, R&D Systems, MN, USA; 1:200), anti-FAP (MAB9727, R&D Systems, MN, USA; 1:200) and anti-PDGF-A (SC-9974,

(7) Mindin/spondin-2 regulates fibroblast subpopulations through distinct Src family kinases during fibrogenesisJCI InsightFebruary 10, 2025Sunny Kataria, Isha Rana, Krithika Badarinath, Rania F. Zaarour, Gaurav Kansagara, Sultan Ahmed, Abrar Rizvi, Dyuti Saha, Binita Dam, Abhik Dutta, et al.al., 2018, ref. 49; Nakasaki et al., 2015, ref. 36); SCA1 (R&D Systems, AF1226); CD26 (R&D Systems, AF954); CD11b (Abcam, ab8878); CD3 (ebiosciences, 14-0032-85), and F4/80 (ebiosciences, 14-4801-81). α-SMA

(8) Response to Commentary on “Structural characterization of SLYM – a 4th meningeal membrane” by Julie Siegenthaler and Christer BetsholtzFluids and Barriers of the CNSSeptember 9, 2024Virginia Plá, Styliani Bitsika, Michael J. Giannetto, Antonio Ladrón-de-Guevara, Daniel Gahn-Martinez, Yuki Mori, Maiken Nedergaard, Kjeld Møllgårdand orange box high magnification insets focus on SLYM and ABCL respectively. (D) DPP4 (R&D Systems, AF954, 1:200) staining shows a distinct immunoreactivity in pia (arrowheads), in the perineurium surrounding

(9) Control of murine brown adipocyte development by GATA6Developmental CellNovember 1, 2023Seoyoung Jun, Anthony R. Angueira, Ethan C. Fein, Josephine M. E. Tan, Angela H. Weller, Lan Cheng, Kirill Batmanov, Jeff Ishibashi, Alexander P. Sakers, Rachel R. Stine, et al.gamma (K.242.9),"Thermo Fisher, MA5–14889",RRID:AB_10985650 Goat anti-DPPIV/CD26,"R and D Systems, AF954",RRID:AB_355739 Sheep anti-Cadherin-4,"R and D Systems, AF6677",RRID:AB_10890570 Anti-PDGFRɑ-PECy7,"Biolegend,

(10) Structural characterization of SLYM - a 4 (th) meningeal membranebioRxiv : the preprint server for biologyOctober 28, 2023Plá V, Bitsika S, Giannetto M, Ladron-de-Guevara A, Gahn-Martinez D, Mori Y, Nedergaard M, Møllgård K.

(11) Defects in microvillus crosslinking sensitize to colitis and inflammatory bowel diseaseEMBO reportsOctober 9, 2023Mödl B, Awad M, Zwolanek D, Scharf I, Schwertner K, Milovanovic D, Moser D, Schmidt K, Pjevac P, Hausmann B, et al.anti‐IAP (Thermo Fisher Scientific #PA5‐22210), anti‐SI (Santa Cruz #sc‐393424), anti‐DPPIV (R&D Systems #AF954), anti‐NHE3 (Novus Biologicals #NBP1‐82574), anti‐p‐ERM (Cell Signaling Technology #3726), anti‐ezrin

(12) Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regenerationCell reportsJune 27, 2023Sun L, Zhang X, Wu S, Liu Y, Guerrero-Juarez CF, Liu W, Huang J, Yao Q, Yin M, Li J, et al.

(13) Pancreas-derived DPP4 is not essential for glucose homeostasis under metabolic stressiScienceMay 19, 2023Fadzeyeva E, Locatelli CAA, Trzaskalski NA, Nguyen MA, Capozzi ME, Vulesevic B, Morrow NM, Ghorbani P, Hanson AA, Lorenzen-Schmidt I, et al.room temperature. Sections were incubated with polyclonal goat anti-mouse DPP4 (1/40, R&D Systems, AF954), recombinant rabbit monoclonal anti-insulin (1/250; Abcam ab181547, clone number EPR17359), and recombinant

(14) Differential Anti-Tumor Effects of IFN-Inducible Chemokines CXCL9, CXCL10, and CXCL11 on a Mouse Squamous Cell Carcinoma Cell LineMedical sciences (Basel, Switzerland)April 25, 2023Matsumoto A, Hiroi M, Mori K, Yamamoto N, Ohmori Y.min. The tissue sections were then incubated with goat anti-mouse DPP4 (CD26) polyclonal antibody (AF954; R&D Systems, Minneapolis, MN, USA) at 4 °C in a humidified chamber for 16 h, washed with TBS for 30

(15) Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue developmentCell reportsMarch 28, 2023Liu Q, Li C, Deng B, Gao P, Wang L, Li Y, Shiri M, Alkaifi F, Zhao J, Stephens JM, et al.anti-Perilipin-1 antibody,Abcam,"Cat# ab61682, RRID:AB_944751" Goat anti-Dpp4 antibody,R & D Systems,"Cat# AF954, RRID:AB_355739" Mouse anti-Icam1 antibody,Novus,Cat# NBP2–22541 Goat anti-Pdgfrα antibody,Novus,"Cat#

(16) Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regenerationCell reportsDecember 20, 2022Mahmud N, Eisner C, Purushothaman S, Storer MA, Kaplan DR, Miller FD.

(17) TGF-β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammationClinical and translational medicineJune 1, 2022Baik JE, Park HJ, Kataru RP, Savetsky IL, Ly CL, Shin J, Encarnacion EM, Cavali MR, Klang MG, Riedel E, et al.human; PA1‐26204 for mouse, 1:1000), collagen III (Proteintech, 22734‐1‐AP, 1:1000), CD26 (R&D Systems, AF954 for mouse, 1:1000; Thermo Fisher Scientific, MA532643 for human, 1:1000), fibronectin (Abcam, ab2413,

(18) Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammationJournal of cell scienceApril 1, 2022Pantasis S, Friemel J, Brütsch SM, Hu Z, Krautbauer S, Liebisch G, Dengjel J, Weber A, Werner S, Bordoli MR.#Ab7778, Abcam, Cambridge, UK), rabbit anti-Ki67 (1:500, #ab15580, Abcam), goat anti-DPPIV/CD26 (1:400, #AF954, R&D Systems), rabbit anti-cleaved caspase 3 (1:400, #9661, Cell Signaling), multilabeling donkey anti-rabbit

(19) Suspension culture promotes serosal mesothelial development in human intestinal organoidsCell reportsFebruary 15, 2022Capeling MM, Huang S, Childs CJ, Wu JH, Tsai YH, Wu A, Garg N, Holloway EM, Sundaram N, Bouffi C, et al.anti-KI67,Thermo Scientific,Cat# RM-9106-S1; RRID: AB_149792 Goat polyclonal anti-DPPIV,R&D Systems,Cat# AF954; RRID: AB_355739 Goat polyclonal anti-LYZ,Santa Cruz Biotechnology,Cat# sc-27958; RRID: AB_2138790 Rabbit

(20) Dpp4+ interstitial progenitor cells contribute to basal and high fat diet-induced adipogenesisMolecular metabolismDecember 1, 2021Stefkovich M, Traynor S, Cheng L, Merrick D, Seale P.following antibodies: anti-GFP (goat, 1:500, Abcam #AB6673), anti-DPP4 (goat; 1:500; R&D Systems # AF954), anti-PLIN1 (rabbit, 1:500, Cell Signaling #3470). Paraffin processing, embedding, sectioning, hematoxylin/eosin

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