Human eNOS Antibody AF950 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman eNOS Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF950
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry
Conjugate/TagUnconjugated
ReactivityGuinea Pig,Human,Porcine
IsotypeIgG
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargeteNOS
HostGoat
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human eNOS
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namenitric oxide synthase 3
NCBI Gene AliasesECNOS, eNOS

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Phenolic Metabolites Protocatechuic Acid and Vanillic Acid Improve Nitric Oxide Bioavailability via the Akt-eNOS Pathway in Response to TNF-α Induced Oxidative Stress and Inflammation in Endothelial CellsMetabolitesNovember 11, 2024Festa J, Hussain A, Al-Hareth Z, Bailey SJ, Singh H, Da Boit M.AF887), p-eNOS (ser1177) (cat. number: MAB9028), total Akt (cat. number: 281046), and eNOS (cat. number: AF950) (all purchased from R&D systems) at a 1:1000 dilution overnight at 4 °C. Following a wash step, the

(2) Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosisFood science & nutritionJuly 1, 2023Festa J, Hussain A, Hackney A, Desai U, Sahota TS, Singh H, Da Boit M.plate (cat: 140675); Nunclon delta surface 12‐well plate (cat: 150628). Western blot antibodies: eNOS AF950, p‐eNOS MAB9028, actin PA1‐46296, akt‐p AF887, akt 281046, NF‐κB MAB5078, NF‐κB p‐p65 72261, Nrf2‐AF3926,

(3) Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathyNature communicationsApril 25, 2023Hussong SA, Banh AQ, Van Skike CE, Dorigatti AO, Hernandez SF, Hart MJ, Ferran B, Makhlouf H, Gaczynska M, Osmulski PA, et al.(Thermo Fisher Scientific R960-25, Waltham, MA, USA), Tau5 (Millipore, MAB361, Billerica, MA, USA)), and/or eNOS (AF950, R&D Systems, Minneapolis, MN, USA) overnight at 4 °C. Cells were incubated with the appropriate secondary

(4) High glutathionylation of placental endothelial nitric oxide synthase in preeclampsiaRedox biologyApril 1, 2019Guerby P, Swiader A, Augé N, Parant O, Vayssière C, Uchida K, Salvayre R, Negre-Salvayre A.anti-iNOS (ab3523) used for immunohistochemistry were from Abcam (Paris, France). Anti-eNOS antibody (AF950) used for immunoprecipitation experiments was from R&D Systems (Bio-Techne, France). Anti-glutathione

(5) Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial CellsMediators of inflammationJanuary 1, 2019Wang Z, Wang F, Kong X, Gao X, Gu Y, Zhang J.

(6) A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in AtherosclerosisFrontiers in immunologyJanuary 1, 2019Noonan J, Grassia G, MacRitchie N, Garside P, Guzik TJ, Bradshaw AC, Maffia P.immunoblotting with antibodies against endothelial nitric oxide synthase (eNOS; R&D Systems, Abingon, UK; Cat# AF950), α-SMA (Agilent, CA, USA; Clone 1A4), and CD11b (R&D systems, Cat# MAB16992). Anti-goat- (Cat# HAF017;

(7) Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF‑αMolecular medicine reportsAugust 1, 2018Liu B, Xu L, Yu X, Li W, Sun X, Xiao S, Guo M, Wang H.no. ab2647; 1:1,000; Abcam, Cambridge, UK), anti-endothelial nitric oxide synthase (eNOS; cat. no. AF950; 1:700; R&D Systems, Inc., Minneapolis, MN, USA), anti-monocyte chemoattractant protein-1 (MCP-1; cat.

(8) Memantine protects against ischemia/reperfusion-induced brain endothelial permeabilityIUBMB lifeApril 1, 2018Liu Y, Huang Y, Xu Y, Qu P, Wang M.

(9) VEGF Receptor-2-Linked PI3K/Calpain/SIRT1 Activation Mediates Retinal Arteriolar Dilations to VEGF and Shear StressInvestigative ophthalmology & visual scienceAugust 1, 2015Hein TW, Rosa RH Jr, Ren Y, Xu W, Kuo L.(1:100, sc-393163; Santa Cruz Biotechnology) and a goat anti-endothelial NOS (eNOS) antibody (1:100, AF950; R&D Systems, Minneapolis, MN, USA). Images were observed using fluorescence microscopy as reported

(10) Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cellsAmerican journal of physiology. Cell physiologyMarch 1, 2010Kawai Y, Yokoyama Y, Kaidoh M, Ohhashi T.

(11) Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compressionBiochemical and biophysical research communicationsJuly 13, 2007Hennerbichler A, Fermor B, Hennerbichler D, Weinberg JB, Guilak F.

(12) Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteriesAmerican journal of respiratory cell and molecular biologyNovember 1, 2004Wright JL, Tai H, Churg A.

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Human eNOS Antibody

eNOS Antibody

eNOS Antibody

eNOS Antibody

eNOS Antibody

Human eNOS Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (12)

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Human eNOS Antibody from R&D Systems, a Bio-Techne Brand

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