MMP-9 Antibody AF911 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMMP-9 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF911
ApplicationsELISA Microarray Development,Zymography,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Proximity Ligation Assay,Immunocytochemistry
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rabbit,Xenograft
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetMMP-9
HostGoat
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human MMP-9
ConcentrationLYOPH
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namematrix metallopeptidase 9
NCBI Gene AliasesCLG4B, GELB, MANDP2, MMP-9
PurityAntigen Affinity-purified

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse MMP-9 in Western blots, dot blots, and human MMP-9 by immunocytochemistry. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.

Sizes Available: 100 ug (also 25 ug)

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Decoupling Mechanical Confinement and Fibrotic Extracellular Matrix Signaling in Vestibular Schwannoma Using Tunable 3D HydrogelsCellular and Molecular BioengineeringMay 15, 2026Melanie Fisher, Han TN. Nguyen, Rinky Ghosh, Yael Vodovotz, Yin Ren1:200), YAP (Abcam, ab56701, 1:200), pFAK (Cell Signaling Technology, Y397, 1:100), MMP9 (R&D Systems, AF911, 1:100), SMAD3 (Cell Signaling Technology, 9523, 1:200), OCT6 (Abcam, ab27925, 1:200), SOX10 (Novus,

(2) (Non)canonical Wnt signaling, cytoarchitecture and stemness: new insights from primary nonmetastatic, primary metastatic, regional and distant metastatic models of adrenocortical carcinomaExperimental & Molecular MedicineNovember 28, 2025Igor Shapiro, Huguette Debaix, Chiara Kräuchi, Andrea Abate, Stefan R. Bornstein, Svenja Nölting, Samuel Gunz, Alfredo Berruti, Sandra Sigala, Felix Beuschlein, et al.SIGMA-ALDRICH (St. Louis, MO, USA)",1/100 Actin,"A2066, SIGMA-ALDRICH (St. Louis, MO, USA)",1/50 MMP9,"AF911, R&D systems (Minneapolis, MN, USA)",1/100 Wnt4,"CSB-PA026137LA01HU, Cusabio (Houston, TX, USA)",1/100 Donkey

(3) Escherichia coli induced matrix metalloproteinase-9 activity and type IV collagen degradation is regulated by progesterone in human maternal decidualBMC Pregnancy and ChildbirthOctober 4, 2024Gerardo Bautista-Bautista, Santos Salguero-Zacarias, Graciela Villeda-Gabriel, Guadalupe García-López, Mauricio Osorio-Caballero, Martha Leticia Palafox-Vargas, Ricardo Josué Acuña-González, Irlando Lara-Pereyra, Oscar Díaz-Ruíz, Hector Flores-Herreraand blocked with 5.0% BSA and 0.05% Tween-20 for 45 min. Next, washed and incubated with anti-MMP-9 (AF911, R&D System) and human collagen-IV (MAB6308, R&D System) primary antibodies at 1:1000 in 1% bovine serum

(4) Ubiquitin-specific protease TRE17/USP6 promotes tumor cell invasion through the regulation of glycoprotein CD147 intracellular traffickingThe Journal of biological chemistrySeptember 1, 2022Ogura Y, Ohbayashi N, Kanaho Y, Kawaguchi A, Funakoshi Y.(ab179751) and anti-LAMP1 (ab24170) antibodies were purchased from Abcam, and goat polyclonal anti-MMP9 (AF911) antibody was purchased from R&D Systems. All Alexa-Fluor-conjugated secondary antibodies were purchased

(5) Splice-switching of the insulin receptor pre-mRNA alleviates tumorigenic hallmarks in rhabdomyosarcomaNPJ precision oncologyJanuary 11, 2022Khurshid S, Montes M, Comiskey DF Jr, Shane B, Matsa E, Jung F, Brown C, Bid HK, Wang R, Houghton PJ, et al.polyvinylidene difluoride membrane, and analyzed for expression of either MMP-9 (1:500; R&D Systems AF911), p-AKT (1:1000; Cell Signaling 4060), pan AKT (1:1000; Cell Signaling 4691), GAPDH (1:7000; Cell Signaling

(6) Acute elevation of interleukin 6 and matrix metalloproteinase 9 during the onset of pituitary apoplexy in Cushing's diseasePituitaryDecember 1, 2021Araki T, Sangtian J, Ruanpeng D, Tummala R, Clark B, Burmeister L, Peterson D, Venteicher AS, Kawakami Y.at room temperature, and stained and rocked overnight with goat polyclonal anti-MMP9 (1:20 dilution; AF911, R&D Systems Inc.) and mouse monoclonal anti-IL-6 (1:10 dilution; MAB206, R&D Systems Inc.). After washing

(7) CD44 modulates metabolic pathways and altered ROS-mediated Akt signal promoting cholangiocarcinoma progressionPloS oneJanuary 1, 2021Thanee M, Dokduang H, Kittirat Y, Phetcharaburanin J, Klanrit P, Titapun A, Namwat N, Khuntikeo N, Wangwiwatsin A, Saya H, et al.were exposed to 3% BSA and incubated with primary antibodies against p21(1:50), MMP-9 (1:200, cat.no. AF911, R&D system, Minneapolis, MN, USA), E-cadherin (1:200), and vimentin (1:200) overnight at 4°C. After

(8) miR‑205 suppresses cell migration, invasion and EMT of colon cancer by targeting mouse double minute 4Molecular medicine reportsAugust 1, 2020Fan Y, Wang K.(MMP)2 (1:1,000; cat. no. MA1‑772; Invitrogen; Thermo Fisher Scientific, Inc.), anti‑MMP9 (1:800; cat. no. AF911; R&D Systems, Inc.) and anti‑GAPDH (1:800; cat. no. AF5718; R&D Systems, Inc.). Following primary incubation,

(9) EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosusJournal of cellular and molecular medicineJanuary 1, 2019Ugarte-Berzal E, Martens E, Boon L, Vandooren J, Blockmans D, Proost P, Opdenakker G.mouse anti‐human C1s MAB2060) and C1r antibodies (AF1807‐SP) and antibodies recognizing human MMP‐9 (AF911) and human MMP‐2 (AF902) were purchased from R&D Systems (Minneapolis, MN, USA). HRP‐conjugated antibodies

(10) Progranulin modulates cholangiocarcinoma cell proliferation, apoptosis, and motility via the PI3K/pAkt pathwayOncoTargets and therapyJanuary 1, 2018Daya M, Loilome W, Techasen A, Thanee M, Sa-Ngiamwibool P, Titapun A, Yongvanit P, Namwat N.(1:100, Cat no 82846; Abcam), and goat polyclonal anti-human metalloproteinase (MMP)-9 (1:1,000, Cat no AF911; R&D Systems, Inc., Minneapolis, MN, USA). Mouse monoclonal anti-beta actin antibody (1:20,000, Cat

(11) Interleukin-6 as a potential molecular target in esophageal squamous cell carcinomaOncology lettersFebruary 1, 2016Zhao ZF, Li JX, Ye R, Wu X, Gao LL, Niu BL.anti-human mouse STAT3 (cat. no. MAB1799; R&D Systems, Inc.) and polyclonal goat anti-human MMP-9 (cat. no. AF911; R&D Systems, Inc.) (1:1,200 dilution) antibodies. The membranes were further incubated with horseradish

(12) TREM-1 Mediates Inflammatory Injury and Cardiac Remodeling Following Myocardial InfarctionCirculation researchMay 22, 2015Boufenzer A, Lemarié J, Simon T, Derive M, Bouazza Y, Tran N, Maskali F, Groubatch F, Bonnin P, Bastien C, et al.mononucleated cells. We detected matrix metalloproteinase 9 (MMP9) with a goat antibody to mouse MMP9 (catalog number AF911, R&D Systems). Cardiac healing after MI was assessed at day 1, 14, and 42. Hearts were excised, rinsed

(13) RAGE mediates S100A7-induced breast cancer growth and metastasis by modulating the tumor microenvironmentCancer researchMarch 15, 2015Nasser MW, Wani NA, Ahirwar DK, Powell CA, Ravi J, Elbaz M, Zhao H, Padilla L, Zhang X, Shilo K, et al.

(14) Anti-vascular endothelial growth factor therapy-induced glioma invasion is associated with accumulation of Tie2-expressing monocytesOncotargetApril 30, 2014Gabrusiewicz K, Liu D, Cortes-Santiago N, Hossain MB, Conrad CA, Aldape KD, Fuller GN, Marini FC, Alonso MM, Idoate MA, et al.instructions. Overnight staining at 4°C with antibodies against Tie2 (C-20, diluted 1:100; Santa Cruz), MMP9 (AF911, diluted 1:50; R&D Systems), mouse Arg1 (V-20, diluted 1:1,000, Santa Cruz). Bound antibodies were detected

(15) Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective studyBMC cancerDecember 13, 2013Thorsen SB, Christensen SL, Würtz SO, Lundberg M, Nielsen BS, Vinther L, Knowles M, Gee N, Fredriksson S, Møller S, et al.prepared by linking a single batch of affinity purified polyclonal antibody (MMP-9 (R&D Systems, Cat.no. AF911) or TIMP-1 (R&D Systems, Cat.no. AF970)) to 3’-hydroxyl free and 5’-phosphate free 40-mer oligonucleotide

(16) Up-regulation of cyclooxygenase 2 and matrix metalloproteinases-2 and -9 in cutaneous squamous cell carcinoma: active role of inflammation and tissue remodeling in carcinogenesisAnnals of dermatologyMay 1, 2013Lee JH, Piao MS, Choi JY, Yun SJ, Lee JB, Lee SC.Biosciences, San Jose, CA, USA), anti-MMP2 (ab809, 1:1000; Chemicon, Billerica, MA, USA), anti-MMP9 (AF911, 1:1,000; R&D Systems, Minneapolis, MN, USA), and anti-β-actin Up-Regulation of CCX-2 and MMPs-2

(17) Pivotal role of matrix metalloproteinase 13 in extracellular matrix turnover in idiopathic pulmonary fibrosisPloS oneJanuary 1, 2013Nkyimbeng T, Ruppert C, Shiomi T, Dahal B, Lang G, Seeger W, Okada Y, D'Armiento J, Günther A.monoclonal antibody to human MMP-8 (1:1000, #IM38L), goat polyclonal antibody to human MMP-9 (1:1000, #AF911, R&D Systems, Minneapolis, MN), monoclonal antibody to latent and active human MMP-13 (1:500, #IM78),

(18) Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humansPloS oneJanuary 1, 2012Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A.

(19) Proteolytic action of kallikrein-related peptidase 7 produces unique active matrix metalloproteinase-9 lacking the C-terminal hemopexin domainsBiochimica et biophysica actaAugust 1, 2011Ramani VC, Kaushal GP, Haun RS.western blot analysis, PVDF membranes were incubated with MMP-9 antibodies generated using full-length proMMP-9 (AF911, R&D Systems), the catalytic domain of MMP-9 (AB19016, Millipore), or peptide immunogens located in

(20) GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in miceNature medicineMay 1, 2011Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, et al.

Be the First to Write a Review!

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody

MMP-9 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (28)

Reviews

MMP-9 Antibody from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map