P-Selectin/CD62P Antibody AF737 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemP-Selectin/CD62P Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF737
NCBI Gene AliasesPADGEM, CD62, Grmp, GMP140, PSEL, CD62P, GRMP, GMP-140, LECAM3
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,In vivo assay,Simple Western,Immunocytochemistry,Functional Assay
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat,Transgenic Mouse
TargetP-Selectin/CD62P
HostGoat
IsotypeIgG
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
NCBI Full Gene Nameselectin, platelet, selectin P
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse P-Selectin/CD62P, Trp42-Ala709, Accession # Q01102
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse P-Selectin/CD62P in direct ELISAs. Detects mouse and rat P-Selectin/CD62P in Western blot. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Effects of Repeated Early-life Anesthesia Exposure on Visual System Development in MiceAnesthesiologyMarch 1, 2026Célia Seillier, Amandine Élodie Bonnet, Shahad Albadri, Nicolas Poirel, Gilles Orliaguet, Denis Vivien, Jean-Philippe SalaünMRI Vascular cell adhesion molecule 1 (VCAM-1),BD Biosciences,553330,Rat,,, P-selectin,R&D Systems,AF737,Goat,,, "IgG2a, κ isotype, clone R35-95",BD Pharmingen,553927,Rat,,, Antibodies used in flow cytometry,Antibodies

(2) Effects of Repeated Early-life Anesthesia Exposure on Visual System Development in MiceAnesthesiologyDecember 4, 2025Célia Seillier, Amandine Élodie Bonnet, Shahad Albadri, Nicolas Poirel, Gilles Orliaguet, Denis Vivien, Jean-Philippe SalaünMRI Vascular cell adhesion molecule 1 (VCAM-1),BD Biosciences,553330,Rat,,, P-selectin,R&D Systems,AF737,Goat,,, "IgG2a, κ isotype, clone R35-95",BD Pharmingen,553927,Rat,,, Antibodies used in flow cytometry,Antibodies

(3) Endothelial cells retain inflammatory memory through chromatin remodeling in a two-hit model of infection-induced inflammationAmerican Journal of Physiology-Cell PhysiologyDecember 1, 2025Daniel Gonsales Gonsales Spindola, Amber Bahr, Samantha Clark, Gabriel Pin Pin de Jesus, Nina Martino, Anthony Lowery, Shuhan Lyu, Andrew Seeman, Grace Martino, Giesse Albeche Albeche Duarte, et al.in PBS-TX with 5% fetal bovine serum (FBS) for 1 h. Primary antibodies—P-Selectin (R&D Systems, Cat# AF737), E-Selectin (ABCAM, Cat# RM2070), VCAM1 (Cell Signaling, Cat# D8U5V), and Isolectin (Thermo Fisher Scientific

(4) From early development to maturity: a phenotypic analysis of the Townes sickle cell disease miceBiol OpenFebruary 15, 2025Ariadna Carol Carol Illa, Henning Hvid, Torben Elm, Christa Andsbjerg Frederiksen, Lonnie Frimodt Bangshof, Dennis Funch Danielsen, Søren Skov, Carsten Dan Leythe primary antibody was blocked in TNB buffer (Roche) for 20 min. A goat anti-P-selectin antibody (#AF737, R&D Systems) was used as the primary antibody at a concentration of 0.2 µg/ml diluted in TNB buffer

(5) LRRC8 complexes are adenosine nucleotide release channels regulating platelet activation and arterial thrombosisbioRxiv : the preprint server for biologySeptember 26, 2024Tranter JD, Mikami RT, Kumar A, Brown G, Abd El-Aziz TM, Zhao Y, Abraham N, Meyer C, Ajanel A, Xie L, et al.

(6) Central nervous system-associated macrophages modulate the immune response following stroke in aged miceNature neuroscienceSeptember 1, 2024Levard D, Seillier C, Bellemain-Sagnard M, Fournier AP, Lemarchand E, Dembech C, Riou G, McDade K, Smith C, McQuaid C, et al.

(7) CNS-associated macrophages contribute to intracerebral aneurysm pathophysiologyActa Neuropathol CommunMarch 18, 2024Martina Glavan, Ana Jelic, Damien Levard, Juhana Frösen, Sara Keränen, Bart A. A. Franx, Ana-Rita Bras, Estelle R. Louet, Ádám Dénes, Mario Merlini, et al.covalently conjugated to purified polyclonal goat anti-mouse antibodies for P-selectin (R&D Systems, clone AF737) and purified polyclonal mouse anti-rat antibodies for VCAM-1 (Clone MR106, BD Pharmingen) were prepared

(8) Transcriptional space-time mapping identifies concerted immune and stromal cell patterns and gene programs in wound healing and cancerCell stem cellJune 1, 2023Hu KH, Kuhn NF, Courau T, Tsui J, Samad B, Ha P, Kratz JR, Combes AJ, Krummel MF.smooth muscle actin antibody (polyclonal),Abcam,AB5694 anti-mouse P-selectin (polyclonal),R&D Systems,AF737 anti-CD31 AlexaFluor647 (clone 390),BioLegend,102416 anti-CD11b AlexaFluor594 (clone M1/70),BioLegend,101254 anti-CD206-AlexaFluor647

(9) JAK-STAT inhibition reduces endothelial prothrombotic activation and leukocyte–endothelial proadhesive interactionsJournal of Thrombosis and HaemostasisMay 1, 2023Joan D. Beckman, Angelica DaSilva, Elena Aronovich, Aithanh Nguyen, Julia Nguyen, Geneva Hargis, David Reynolds, Gregory M. Vercellotti, Brian Betts, David K. WoodCell Signaling Technology. The following antibodies were used for immunofluorsence: P-selectin (R&D Systems #AF737), VWF (vWF, Cedarlane #CL20176A-R), and VE-cadherin (R&D Systems #AF1002). 2.3 | Endothelial cell culture Primary

(10) Adhesion analysis via a tumor vasculature-like microfluidic device identifies CD8(+) T cells with enhanced tumor homing to improve cell therapyCell reportsMarch 28, 2023Camargo CP, Muhuri AK, Alapan Y, Sestito LF, Khosla M, Manspeaker MP, Smith AS, Paulos CM, Thomas SN.incubated overnight at 4°C with the following primary antibody: goat anti-mouse CD62P (1:13, R&D Systems, AF737), and rat anti-mouse CD3 (1:50, Invitrogen) or rat anti-mouse CD31 (1:50, Invitrogen). The following

(11) Angiopoietin-1 Is Required for Vortex Vein and Choriocapillaris Development in MiceArteriosclerosis, Thrombosis, and Vascular BiologyNovember 1, 2022Pan Liu, Jeremy A. Lavine, Amani Fawzi, Susan E. Quaggin, Benjamin R. Thomson(Cell Signaling #2880), chicken anti GFP 1:2000 (Abcam Ab13970), goat anti P-Selectin 1:100 (R&D Systems AF737). Complete antibody information is provided in the attached major resources table. For low-magnification

(12) Angiopoietin-1 is required for vortex vein and choriocapillaris development in miceArterioscler Thromb Vasc BiolSeptember 29, 2022Pan Liu, Jeremy A. Lavine, Amani Fawzi, Susan E Quaggin, Benjamin R. Thomson(Cell Signaling #2880), chicken anti GFP 1:2000 (Abcam Ab13970), goat anti P-Selectin 1:100 (R&D Systems AF737). Complete antibody information is provided in the attached major resources table. For low-magnification

(13) Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activationCell death & diseaseSeptember 22, 2022Marona P, Górka J, Kwapisz O, Jura J, Rys J, Hoffman RM, Miekus K.Pharmingen, San Jose, CA, USA, 4163758 and 4234535); anti-selectin P (SelP) antibody (10 µg/ml, R&D Systems, AF737) or anti-ICAM-1 antibody (1:100, eBioscience, Affymetrix, USA, 14-0542-82) in 1% BSA in PBS. On the

(14) Humanized Anti-RGMa Antibody Treatment Promotes Repair of Blood-Spinal Cord Barrier Under Autoimmune Encephalomyelitis in MiceFrontiers in immunologyJanuary 1, 2022Hirata T, Itokazu T, Sasaki A, Sugihara F, Yamashita T.Afterwards, spinal cord was serially sectioned into 20-μm transverse sections. Goat anti-P-selectin (1:1,000, AF737; R&D Systems), rabbit anti-collagen 1 (1:100, ab34710; Abcam), goat anti-collagen 4 (1:50, AB769; Sigma-Aldrich),

(15) A cellular and spatial map of the choroid plexus across brain ventricles and agesCellMay 27, 2021Dani N, Herbst RH, McCabe C, Green GS, Kaiser K, Head JP, Cui J, Shipley FB, Jang A, Dionne D, et al.Biolegend), Rat anti-CD31/PECAM1 (1:200, 550274, BD Pharmingen), Goat anti-CD62P/P-Selectin (1:100, AF737, R&D Systems), Mouse anti-CLDN5-488 (1:400, 331588, Invitrogen), Rabbit anti-COL1A1 (1:250, AB34710,

(16) Disrupting tumour vasculature and recruitment of aPDL1-loaded platelets control tumour metastasisNature communicationsMay 13, 2021Li H, Wang Z, Chen Z, Ci T, Chen G, Wen D, Li R, Wang J, Meng H, Bryan Bell R, et al.(BioLegend, catalogue no. 124308, clone: 10F.9G2, PE-conjugated), CD62P (R&D Systems, catalogue no. AF737, goat IgG), CD31 (R&D Systems, catalogue no. AF3628, goat IgG), Donkey anti-goat IgG (Invitrogen, catalogue

(17) Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNSThe Journal of clinical investigationApril 1, 2020Li Z, Korhonen EA, Merlini A, Strauss J, Wihuri E, Nurmi H, Antila S, Paech J, Deutsch U, Engelhardt B, et al.rat anti-mouse TER-119 (clone TER-119, BD Pharmingen), and goat anti-P-selectin (R&D Systems, catalog AF737). After washing with PBS containing 0.1–0.3% Triton X-100, sections were stained with fluorophore-conjugated

(18) Carfilzomib Delivery by Quinic Acid-Conjugated Nanoparticles: Discrepancy Between Tumoral Drug Accumulation and Anticancer Efficacy in a Murine 4T1 Orthotopic Breast Cancer ModelJournal of pharmaceutical sciencesApril 1, 2020Jun Y, Xu J, Kim H, Park JE, Jeong YS, Min JS, Yoon N, Choi JY, Yoo J, Bae SK, et al.(Torrance, CA, USA). Antibodies against E-selectin (catalog No. AF575) and P-selectin (catalog No. AF737) were from R&D systems (Minneapolis, MN, USA). All other reagents were from Thermo Fisher Scientific

(19) Alcohol exposure-induced neurovascular inflammatory priming impacts ischemic stroke and is linked with brain perivascular macrophagesJCI insightFebruary 27, 2020Drieu A, Lanquetin A, Levard D, Glavan M, Campos F, Quenault A, Lemarchand E, Naveau M, Pitel AL, Castillo J, et al.covalently conjugated to purified polyclonal goat anti-mouse antibodies for P-selectin (R&D Systems, clone AF737) were prepared as previously described (46). Details are provided in the Supplemental Methods. In vivo

(20) Endothelial TLR4 Expression Mediates Vaso-Occlusive Crisis in Sickle Cell DiseaseFrontiers in immunologyJanuary 1, 2020Beckman JD, Abdullah F, Chen C, Kirchner R, Rivera-Rodriguez D, Kiser ZM, Nguyen A, Zhang P, Nguyen J, Hebbel RP, et al.microtome-cryostat into 6 µm sections. Tissues were stained with primary antibodies to P-selectin (R&D Systems #AF737) and von Willebrand factor (vWF, Cedarlane #CL20176A-R), and with the nuclear stain DAPI (Sigma-Aldrich).

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P-Selectin/CD62P Antibody

P-Selectin/CD62P Antibody

P-Selectin/CD62P Antibody

P-Selectin/CD62P Antibody

P-Selectin/CD62P Antibody

P-Selectin/CD62P Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (32)

Reviews

P-Selectin/CD62P Antibody from R&D Systems, a Bio-Techne Brand

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