STAT5b Antibody AF1584 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSTAT5b Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $399.00Supplier Page View Company Product Page
Catalog NumberAF1584
HostRabbit
Conjugate/TagUnconjugated
Quantity50 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ReactivityHuman,Mouse
TargetSTAT5b
ClonalityPolyclonal
ImmunogenHuman STAT5b synthetic peptide, aa 777-787
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesGHISID2, STAT5
NCBI Full Gene Namesignal transducer and activator of transcription 5B
ApplicationsWB, FCM, ICC, IHC, IP, ChIP, MC

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse STAT5b.

Sizes Available: 50 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Disease-associated mutations in the STAT5B SH2 domain reprogram hepatic cholesterol and lipid metabolismbioRxiv : the preprint server for biologyDecember 1, 2025Lee HK, Pyatkov M, Gavrilova O, Liu N, Demby T, Ye B, Furth PA, Hennighausen L, Waxman DJ.immunoprecipitated with Dynabeads Protein A (Novex) coated with ChIP-seq grade antibodies: STAT5B (R&D systems, AF1584; ThermoFisher scientific, 13–5300), H3K27ac (Abcam, ab4729) and RNA polymerase II (Abcam, ab5408). Libraries

(2) STAT5B leukemic mutations, altering SH2 tyrosine 665, have opposing impacts on immune gene programsLife Sci AllianceApril 14, 2025Hye Kyung Lee, Jichun Chen, Rachael L Philips, Sung-Gwon Lee, Xingmin Feng, Zhijie Wu, Chengyu Liu, Aaron B Schultz, Molly Dalzell, Manja Meggendorfer, et al.previously described (Metser et al, 2016). The following antibodies were used for ChIP-seq: STAT5B (AF1584; R&D Systems; 13-5300; Thermo Fisher Scientific), H3K27ac (ab4729; Abcam), and RNA polymerase II (ab5408;

(3) Disease-Associated Mutations of the STAT5B SH2 Domain Regulate Cytokine-Driven Enhancer Function and Mammary DevelopmentJ Mammary Gland Biol NeoplasiaMarch 31, 2025Hye Kyung Lee, Jakub Jankowski, Chengyu Liu, Lothar Hennighausenwith ChIP-seq grade antibodies: STAT5A (Santa Cruz Biotechnology, sc-271542), STAT5B (R&D systems, AF1584; ThermoFisher scientific, 13-5300), NFIB (Sigma-Aldrich, HPA003956), H3K27ac (Abcam, ab4729) and RNA

(4) STAT5B leukemic mutations, altering SH2 tyrosine 665, have opposing impacts on immune gene programsbioRxivDecember 22, 2024Hye Kyung Lee, Jichun Chen, Rachael L. Philips, Sung-Gwon Lee, Xingmin Feng, Zhijie Wu, Chengyu Liu, Aaron B. Schultz, Molly Dalzell, Foster Birnbaum, et al.antibodies were used for ChIP-seq: STAT5A (Santa Cruz Biotechnology, sc-271542), STAT5B (R&D systems, AF1584; ThermoFisher scientific, 13–5300), H3K27ac (Abcam, ab4729) and RNA polymerase II (Abcam, ab5408). Libraries

(5) STAT5B SH2 variants disrupt mammary enhancers and the stability of genetic programs during pregnancybioRxivMay 14, 2024Hye Kyung Lee, Chengyu Liu, Lothar Hennighausenantibodies were used for ChIP-seq: STAT5A (Santa Cruz Biotechnology, sc-271542), STAT5B (R&D systems, AF1584; ThermoFisher scientific, 13-5300), H3K27ac (Abcam, ab4729) and RNA polymerase II (Abcam, ab5408). Libraries

(6) Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein MarkersBiosensorsAugust 27, 2023Meah A, Vedarethinam V, Bronstein R, Gujarati N, Jain T, Mallipattu SK, Li Y, Wang J.

(7) Human signal transducer and activator of transcription 5b (STAT5b) mutation causes dysregulated human natural killer cell maturation and impaired lytic functionThe Journal of allergy and clinical immunologyJanuary 1, 2020Vargas-Hernández A, Witalisz-Siepracka A, Prchal-Murphy M, Klein K, Mahapatra S, Al-Herz W, Mace EM, Carisey AF, Orange JS, Sexl V, et al.nitrocellulose membrane (Invitrogen). The following primary antibodies were used: rabbit polyclonal anti-STATb (AF1584, R&D Systems), mouse monoclonal anti-STAT5a (clone 4H1, Cell Signaling Technologies), and mouse anti-beta

(8) AMPK Alpha-1 Intrinsically Regulates the Function and Differentiation of Tumor Myeloid-Derived Suppressor CellsCancer researchOctober 1, 2019Trillo-Tinoco J, Sierra RA, Mohamed E, Cao Y, de Mingo-Pulido Á, Gilvary DL, Anadon CM, Costich TL, Wei S, Flores ER, et al.D2O6Y, Cell Signaling Technologies), Stat-5a (Rabbit monoclonal E289, Abcam), Stat-5b (Rabbit polyclonal AF1584, R&D systems), or Vinculin (Clone hVIN-1, Sigma-Aldrich) (All at 1:1000). Horseradish peroxidase linked

(9) Retinoic Acid Receptor Alpha Represses a Th9 Transcriptional and Epigenomic Program to Reduce Allergic PathologyImmunityJanuary 15, 2019Schwartz DM, Farley TK, Richoz N, Yao C, Shih HY, Petermann F, Zhang Y, Sun HW, Hayes E, Mikami Y, et al.Diagenode), anti-NRIP1 (ab42126; Abcam), a 1:1 mix of anti-STAT5A(PA-ST5A; R&D Systems)/anti-STAT5B (AF1584, R&D Systems), anti-STAT6 (ab32520; Abcam), or anti-CTCF (07–729; Millipore). Aliquots of genomic DNA

(10) TSLP signaling in CD4(+) T cells programs a pathogenic T helper 2 cell stateScience signalingMarch 13, 2018Rochman Y, Dienger-Stambaugh K, Richgels PK, Lewkowich IP, Kartashov AV, Barski A, Khurana Hershey GK, Leonard WJ, Singh H.monoclonal anti-H3K4me3 antibody (17-614, Millipore), or a rabbit polyclonal anti-STAT5A/5B antibody (AF1584, R&D Systems) and Dynabeads Protein G (Invitrogen) in radioimmunoprecipitation assay buffer (10 mM tris-EDTA,

(11) Critical functions for STAT5 tetramers in the maturation and survival of natural killer cellsNature communicationsNovember 6, 2017Lin JX, Du N, Li P, Kazemian M, Gebregiorgis T, Spolski R, Leonard WJ.immunoprecipitated with control rabbit IgG (sc-3888, Santa Cruz Biotechnology, Dallas TX) or anti-STAT5B (AF1584, R&D Systems) and Magna ChIP Protein A + G Magnetic Beads (16–663, Millipore, Billerica MA). ChIP-Seq

(12) BCR-ABL affects STAT5A and STAT5B differentiallyPloS oneJanuary 1, 2014Schaller-Schönitz M, Barzan D, Williamson AJ, Griffiths JR, Dallmann I, Battmer K, Ganser A, Whetton AD, Scherr M, Eder M.(611835), BCL-x (610211) from BD Transduction; STAT5B from BD Pharmingen (556517) or from R&D Systems (AF1584) and phospho-STAT5 (Tyr694) (C11C5), phospho-JAK2 (Tyr1007/1008) (C80C3), phospho-GAB2 (Tyr452) (C33G1),

(13) Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factorsThe Journal of cell biologyDecember 18, 2006Kawashima T, Bao YC, Nomura Y, Moon Y, Tonozuka Y, Minoshima Y, Hatori T, Tsuchiya A, Kiyono M, Nosaka T, et al.

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STAT5b Antibody

STAT5b Antibody

STAT5b Antibody

STAT5b Antibody

STAT5b Antibody

STAT5b Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (13)

Reviews

STAT5b Antibody from R&D Systems, a Bio-Techne Brand

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