TIMP-1 Antibody AF980 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTIMP-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF980
NCBI Gene AliasesTIMP, TPA-S1, TIMP-1, Timp, EPO, CLGI, Clgi, HCI, EPA
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Electron Microscopy,In vivo assay,Neutralization,Immunocytochemistry
Quantity100 ug (also 25 ug)
IsotypeIgG
TargetTIMP-1
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Transgenic Mouse
HostGoat
NCBI Full Gene Nametissue inhibitor of metalloproteinase 1, TIMP metallopeptidase inhibitor 1
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse TIMP-1, Cys25-Arg205, Accession # P12032
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this TIMP-1 antibody be used for Western blotting in mouse models?

Yes, the TIMP-1 antibody has been successfully utilized in Western blotting applications, as demonstrated in studies where it was used to analyze TIMP-1 expression in various mouse models, including those investigating tumor proliferation and inflammation responses (, ).

Is this antibody effective for immunohistochemistry in tissue samples?

The TIMP-1 antibody has been shown to work effectively in immunohistochemistry, as evidenced by its application in studies examining tissue samples from mouse models, where it was used to assess TIMP-1 expression in lung tissues and other relevant samples (, ).

What species does this antibody react with, and how specific is it?

This TIMP-1 antibody is reactive with mouse and human TIMP-1. It exhibits approximately 15% cross-reactivity with recombinant rat TIMP-1 and less than 5% with recombinant human TIMP-1, indicating a high level of specificity for mouse TIMP-1 ().

Are there any notable applications of this antibody in recent research?

Recent studies have highlighted the role of TIMP-1 in various biological processes, including its involvement in tumor cell proliferation mediated by PECAM-1 interactions (), and its anti-angiogenic properties in mesenchymal stem cell therapy (). These applications underscore the antibody's relevance in current research contexts.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Cold and hot fibrosis define clinically distinct cardiac pathologiesCell SystemsMarch 19, 2025Shoval Miyara, Miri Adler, Kfir B. Umansky, Daniel Häußler, Elad Bassat, Yalin Divinsky, Jacob Elkahal, David Kain, Daria Lendengolts, Ricardo O. Ramirez Flores, et al.used for staining. anti-LRP1 (cell culture, 1:100, ab92544, Abcam), anti-TIMP1 (cell culture, 1:50, AF980, R&D systems), anti-aSMA (cell culture & tissue sections, 1:400, A2547, Sigma), Anti-GFP (tissue sections,

(2) SLPI deficiency alters airway protease activity and induces cell recruitment in a model of muco-obstructive lung diseaseFrontiers in ImmunologySeptember 5, 2024Ryan Brown, Caoifa Dougan, Peter Ferris, Rebecca Delaney, Claire J. Houston, Aoife Rodgers, Damian G. Downey, Marcus A. Mall, Bronwen Connolly, Donna Small, et al.Healthcare, Buckinghamshire, UK). Membranes were blocked and incubated with TIMP-1 antibody (Bio-techne (AF980) 1:2000) or SLPI antibody (R&D Systems (AF1735), 1:2000) overnight at 4°C. Membranes were washed, incubated

(3) Elevated matrix Metalloproteinase-9 associated with reduced cerebellar perineuronal nets in female mice with toxoplasmosisBrain, Behavior, & Immunity - HealthMarch 1, 2024Jianchun Xiao, Jing Huang, Robert H. Yolken(polyclonal, AF909, 1: 800, R&D systems), NGAL (polyclonal, ab63929, 1:500, Abcam), TIMP-1 (polyclonal, AF980, 1:500, R&D systems), synaptophysin (monoclonal, ab8049, 1:500, Abcam), pGSK-3α/β (Ser21/9) (polyclonal,

(4) Astrocytic TIMP-1 regulates production of Anastellin, an inhibitor of oligodendrocyte differentiation and FTY720 responsesProceedings of the National Academy of Sciences of the United States of AmericaJanuary 30, 2024Sutter PA, Willis CM, Menoret A, Nicaise AM, Sacino A, Sikkema AH, Jellison ER, Win KK, Han DK, Church W, et al.1:100-immunodepletion), Anti-Fibronectin antibody (ab2033) (Abcam; rabbit, 1:200), Anti-Timp1 antibody (AF980) (R&D Systems; goat, 1:125), Anti-MBP antibody (12) (Millipore; rat monoclonal, 1:500), Mouse anti-A2B5

(5) Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma CellsInternational journal of molecular sciencesNovember 28, 2023Varga AJ, Nemeth IB, Kemeny L, Varga J, Tiszlavicz L, Kumar D, Dodd S, Simpson AWM, Buknicz T, Beynon R, et al.AF902 and MAB973, respectively), TIMP-1, TIMP-2, prosaposin, and MMP-1 (R&D Systems, Abingdon, UK; AF980, AF971, AF8520, and MAB901, respectively) were utilized. A densitometric analysis of the immunoblots

(6) HMGB2 is a potential diagnostic marker and therapeutic target for liver fibrosis and cirrhosisHepatol CommunNovember 6, 2023Yi Huang, Suthat Liangpunsakul, Swetha Rudraiah, Jing Ma, Santosh K. Keshipeddy, Dennis Wright, Antonio Costa, Diane Burgess, Yuxia Zhang, Nazmul Huda, et al.p-Smad2/3 (#8828), Lamin A/C (#4777), and α-SMA (#14968). Antibodies against metalloproteinases (MMP)2 (#AF980), MMP9 (#AF1488), and TIMP1 (#AF909) were obtained from R&D Systems (Minneapolis, MN). An anti-HA antibody

(7) HMGB2 is a potential diagnostic marker and therapeutic target for liver fibrosis and cirrhosisHepatology CommunicationsNovember 6, 2023Yi Huang, Suthat Liangpunsakul, Swetha Rudraiah, Jing Ma, Santosh K. Keshipeddy, Dennis Wright, Antonio Costa, Diane Burgess, Yuxia Zhang, Nazmul Huda, et al.p-Smad2/3 (#8828), Lamin A/C (#4777), and α-SMA (#14968). Antibodies against metalloproteinases (MMP)2 (#AF980), MMP9 (#AF1488), and TIMP1 (#AF909) were obtained from R&D Systems (Minneapolis, MN). An anti-HA antibody

(8) Regorafenib prevents the development of emphysema in a murine elastase modelBMB reportsAugust 1, 2023Oh K, Lee GW, Kim HB, Park JH, Shin EY, Kim EG.(Cambridge, UK), CXCL1 antibody (12335-1-AP) from Proteintech (Rosemont, IL, USA), and TIMP-1 antibody (#AF980) from R&D Systems were purchased respectively. Mouse inflammation antibody array (Cat # ab133999) was

(9) Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-γInflammationJune 1, 2022Coleman CS, Stanley BA, Lang CH.polyclonal, 0.1 μg/ml (AF365), VCAM1 goat polyclonal, 1 μg/ml (AF643), TIMP1 goat polyclonal, 0.1 μg/ml (AF980) were from R&D Systems, Minneapolis, MN). For Western analysis, representative blots are from a single

(10) Ym1⁺ macrophages orchestrate fibrosis, lesion growth, and progression during development of murine pancreatic canceriScienceMay 20, 2022Fleming Martinez AK, Döppler HR, Bastea LI, Edenfield BH, Liou GY, Storz P.smooth muscle actin,Abcam,"Cat# ab5694, RRID:AB_2223021" Goat polyclonal anti-TIMP1,R&D Systems,Cat# AF980; RRID:AB_355759 Rabbit polyclonal anti-Y369-TGFβ1,Boster Biological Technology,Cat# A00019Y369; RRID:AB_2904551 Goat

(11) Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activationFrontiers in cellular neuroscienceJanuary 1, 2022Piekna-Przybylska D, Na D, Zhang J, Baker C, Ashton JM, White PM.EMD Millipore; MAB1572), goat anti-OPN (SPP1; 1:100; R&D Systems; AF808), goat anti-TIMP1 (1:100; R&D Systems; AF980), rabbit anti-CD44 (1:100; Abcam; AB157107), rabbit anti-pERBB2 (1:200; Santa Cruz; sc-12352), goat

(12) Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine β-coronavirus-induced neuroinflammationVirologyJanuary 1, 2022Sengupta S, Addya S, Biswas D, Banerjee P, Sarma JD.inhibitor cocktail (Cat:11836170001) was from Roche Diagnostics, Germany. Mouse TIMP-1 antibody (Cat: AF980) was obtained from R&D Systems, USA. Mouse MMP-8 antibody (Cat: SAB4501895) was purchased from Sigma-Aldrich,

(13) TIMP1 expression underlies sex disparity in liver metastasis and survival in pancreatic cancerThe Journal of experimental medicineNovember 1, 2021Hermann CD, Schoeps B, Eckfeld C, Munkhbaatar E, Kniep L, Prokopchuk O, Wirges N, Steiger K, Häußler D, Knolle P, et al.

(14) Myeloid-Derived TSP1 (Thrombospondin-1) Contributes to Abdominal Aortic Aneurysm Through Suppressing Tissue Inhibitor of Metalloproteinases-1Arteriosclerosis, thrombosis, and vascular biologyDecember 1, 2020Yang H, Zhou T, Sorenson CM, Sheibani N, Liu B.10% normal donkey serum and incubated at 4 °C overnight with primary antibodies anti- TIMP1 (1:100, AF980, R&D Systems), anti- MYH11 (1:200, ab53219, Abcam), PE anti-mouse F4/80 (1:100, 123110, clone BM8, BioLegend).

(15) CD73⁺ extracellular vesicles inhibit angiogenesis through adenosine A_2B receptor signallingJournal of extracellular vesiclesJanuary 1, 2020Angioni R, Liboni C, Herkenne S, Sánchez-Rodríguez R, Borile G, Marcuzzi E, Calì B, Muraca M, Viola A.clone KMC8), anti-CD39 (Biolegend, clone Duha59), anti-CD73 (Abcam, ab175396) and TIMP-1 (R&D systems AF980) antibodies were used at the dilution 1:1000. Thus, membranes were incubated with the appropriate peroxidase-conjugated

(16) In Vivo Activation and Pro-Fibrotic Function of NF-κB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon NanotubesFrontiers in pharmacologyJanuary 1, 2019Dong J, Ma Q.p65 S276 (Santa Cruz, sc-101749, rabbit polyclonal), anti-TIMP1 (R&D systems, Minneapolis, MN, USA, AF980, goat polyclonal), and anti-OPN (R&D systems, AF808, goat polyclonal) antibodies. All primary antibodies

(17) Orthologous proteins of experimental de- and remyelination are differentially regulated in the CSF proteome of multiple sclerosis subtypesPloS oneJanuary 1, 2018Martin NA, Nawrocki A, Molnar V, Elkjaer ML, Thygesen EK, Palkovits M, Acs P, Sejbaek T, Nielsen HH, Hegedus Z, et al.

(18) Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasisOncogeneAugust 31, 2017Giebeler N, Schönefuß A, Landsberg J, Tüting T, Mauch C, Zigrino P.

(19) Mouse mesenchymal stem cells inhibit high endothelial cell activation and lymphocyte homing to lymph nodes by releasing TIMP-1LeukemiaMay 1, 2016Zanotti L, Angioni R, Calì B, Soldani C, Ploia C, Moalli F, Gargesha M, D'Amico G, Elliman S, Tedeschi G, et al.inhibitor of metalloproteinase-1 immunoneutralization Goat polyclonal anti-TIMP-1 IgG19 (catalog no. AF980; R&D Systems, Minneapolis, MN, USA) was intravenously (i.v.) administered (0.5 mg/kg) in immunized mice

(20) Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in MiceThe Journal of biological chemistryApril 8, 2016Fan MH, Zhu Q, Li HH, Ra HJ, Majumdar S, Gulick DL, Jerome JA, Madsen DH, Christofidou-Solomidou M, Speicher DW, et al.

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6/27/2017Excellent Antibody Against Murine TIMP-1Dr. Elena DeryuginaThe AF980 goat polyclonal antibody AF98- from R&D Systems can be successfully used to detect murine TIMP-1 by western blotting.

TIMP-1 Antibody

TIMP-1 Antibody

TIMP-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (33)

Reviews

TIMP-1 Antibody from R&D Systems, a Bio-Techne Brand

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