CD31/PECAM-1 Antibody AF806 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD31/PECAM-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF806
ApplicationsFACS,Flourescense Microscopy,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry, ELISA Capture
Quantity100 ug (also 25 ug)
TargetCD31/PECAM-1
IsotypeIgG
ReactivityBovine,Human,Mouse,Primate,Primate - Macaca fuscata (Japanese Macaque),Primate - Macaca mulatta (Rhesus Macaque),Rabbit,Transgenic Mouse,Xenograft
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
ValidationKnockout Validated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesCD31, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, endoCAM
NCBI Full Gene Nameplatelet and endothelial cell adhesion molecule 1
ConcentrationLYOPH
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human CD31, Extracellular domain
ClonalityPolyclonal
HostSheep
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study endothelial cell behavior in the context of vascular remodeling?

Yes, the CD31/PECAM-1 antibody has been utilized in studies examining endothelial cell interactions and behaviors, particularly in the context of vascular remodeling. For instance, it was used to assess endothelial cell characteristics in research focused on the role of immune cells in vascular integrity and angiogenesis in glioblastoma models, highlighting its relevance in vascular studies (Yao et al., 2024).

Is this antibody effective for flow cytometry applications?

The CD31/PECAM-1 antibody has been shown to work effectively in flow cytometry applications. It was employed in a study to analyze extracellular vesicle populations, demonstrating its utility in characterizing cell surface markers associated with endothelial cells and other immune cell types (Brahmer et al., 2019).

What is the specificity of this antibody for human CD31?

The CD31/PECAM-1 antibody specifically detects human CD31 with less than 5% cross-reactivity with recombinant mouse CD31 in direct ELISAs, making it a reliable choice for studies focused on human endothelial cells (R&D Systems).

Has this antibody been used in any studies related to inflammation and immune cell migration?

Yes, the CD31/PECAM-1 antibody has been referenced in studies investigating the mechanisms of neutrophil transendothelial migration, where it was shown to co-cluster with L-selectin during the migration process, indicating its role in regulating immune responses (Rahman et al., 2021).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular DysfunctionJ Histochem CytochemMay 11, 2026Paola Serrano Martinez, Maxime Cammeraat, Amber Teppema, Cornelis J. F. van Noorden, Reinier O. Schlingemann, Ingeborg KlaassenAntibody,Working Dilution,Identifier,Manufacturer Anti-CD31,1:50,AF806,R&D systems Anti-PDGFRβ,1:100,AF385,R&D systems Anti-CD34,1:100,M0014,Sanquin Anti-α-SMA,1:100,M0851,Agilent

(2) A Biphasic Effect of Alcohol on Endothelial Plasticity Through Regulation of Endothelial-to-Mesenchymal TransitionbioRxivApril 20, 2026Weimin Liu, Yusof Gusti, Fathima Athar, Naresh K Rajendran, Paul A. Cahill, Eileen M. RedmondHD, Pikachu). Images were analyzed using Image J. Primary antibodies used include human CD31 (Cat. # AF806, R&D Systems), αSMA (MAB1420, R&D systems), SM22α (Cat. # 36090 CST), and CDH5 (Cat. # 2500, CST). Secondary

(3) A 3D microfluidic model of exchange between perfused blood and lymphatic microvascular networksLab on a ChipApril 7, 2026Delaney Gray-Scherr, Terry Ching, Katherine Beran, Sarah C. Adams, Emily Davis, Abraham C. I. van Steen, Trisha Raman, Wilson W. Wong, Jeroen Eyckmans, Christopher S. Chen(BioLegend, 916606), rabbit anti-human PROX1 (Abcam, ab209016), sheep anti-human CD31/PECAM-1 (R&D Systems, AF806), rabbit anti-human VE-cadherin (Cell Signaling Technology, 2500), chicken anti-GFP (Abcam, ab13970),

(4) Plasma Extracellular Vesicle Modulate Immune Cell Transcriptional Responses Following Acute Myocardial InfarctioniScienceMarch 20, 2026Lewis Timms, Raphaella Ridley, Adam Braithwaite, Charlotte Hooper, Tanvir Rafe, Carolin Lewe, Sebastian Lemke, Divya Ganesh, Zeyu Chen, Tafadzwa Kufazvinei, et al.,BD Biosciences,555426; RRID:AB_395820 CD3,BD Biosciences,555337; RRID:AB_395743 CD31 (PECAM-1),R & D Systems,AF806; RRID:AB_355617 CD36,Santa Cruz,sc-7309; RRID:AB_627044 CD4,R & D Systems,MAB379; RRID:AB_2244289 CD41,Biolegend,303702;

(5) Paracrine Factor Local Gradient‐Generating System for Engineering Perfusable Vascularized Hepatocyte Tissues with Perfusion‐Induced ProliferationAdvanced Healthcare MaterialsMarch 16, 2026Yen‐Hsiang Huang, Tadahiro Yamashita, Ryo SudoSamples were then incubated overnight at 4°C with the following primary antibodies: sheep anti‐CD31 (AF806, R&D Systems, Minneapolis, MN, USA) to label HUVECs; mouse anti‐MRP2 (NBP1‐42349, Novus Biologicals,

(6) Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilienceJournal of Clinical InvestigationMarch 2, 2026Myung Jin Yang, Seok Kang, Seon Pyo Hong, Hokyung Jin, Jin-Hui Yoon, Cheolhwa Jin, Chae Min Yuk, Lydia Getachew Gebeyehu, Junho Jung, Sung-Hwan Yoon, et al.anti-VEGFR3/Flt4 (MAB3491); sheep anti-FoxC2 (AF5044); goat anti-VEGFR2/Flk-1 (AF357); sheep anti-CD31 (AF806); goat anti-GATA2 (AF2046) (R&D Systems); mouse anti-NFATC1 (MA3-024, Invitrogen); and rabbit anti-GAPDH

(7) A vascularized liver microphysiological system captures key features of hepatic insulin resistance and monocyte infiltrationNat CommunFebruary 3, 2026Erin N. Tevonian, Ellen L. Kan, Kairav K. Maniar, Alex J. Wang, Anisha Datta, Roger D. Kamm, Douglas A. Lauffenburger, Linda G. Griffithat 4 °C. Samples were then incubated with some combination of primary antibodies for CD31 at 1:100 (AF806, Bio-Techne, lot # DGX0523031), CD68 at 1:200 (14-0688-82, Invitrogen, lot # TE2570551), CD163 at 1:100

(8) Methodological and Short-Term Diurnal Variation in Surface and Cargo Proteins in Plasma Extracellular VesiclesCurrent Issues in Molecular BiologyJanuary 22, 2026Hubert Krzyslak, Weronika Maria Szejniuk, Ursula Falkmer, Bent Honoré, Malene Møller Jørgensen, Charlotte Sten, Shona Pedersen, Gunna Christiansen, Søren Risom KristensenHLA DR/DP/DQ (Capricobio, Norcross, GA, USA, Cat.# 104701 Clone: HB-145/IVA12), CD31 (R&D Systems, Cat.# AF806, Polyclonal, TNF RII (R&D Systems, Cat.# MAB726 Clone: 22210) and TNF RI (R&D Systems, Cat.# MAB225,

(9) Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot studyBMC NeuroscienceJanuary 12, 2026Rebecca Best Jensen, Maria Kjølhede, Jesper Just, Lee-Ann Clegg, Rikke Baek, Erik Kaadt, Betina Elfving, David C. Hess, Grethe Andersen, Malene Møller Jørgensen, et al.Biosciences,"559,043",3G8 VEGFR2,Biolegend,"359,902",7D4-6 tPA,R & D Systems,AF7449,- CD31 (PECAM-1),R & D Systems,AF806,- VE-Cadherin,R & D Systems,AF938,- CD105 (Endoglin),LS Bio,LS-C149182,- Glypican-1,R & D Systems,AF4519,- CD235a(glycophorin A

(10) NLRP3/GSDMD-dependent neutrophil extracellular traps exacerbate microglia-mediated neuroinflammation following traumatic brain injuryCell Communication and SignalingJanuary 12, 2026Liang Liu, Bingyou Yuan, Yuhua Wang, Jianpeng Liu, Peiyu Li, Haifeng Zhang, Xian Zhang, Jianhao Wang, Yan Chai, Quanjun Deng, et al.anti-ZO-1 (1:5000, ab276131, Abcam, UK), goat anti-IBA-1 (1:500, ab5076, Abcam, UK), goat anti-CD31 (1:200, AF806, R&D Systems), goat anti-MPO (1:500, AF3667, R&D Systems), rabbit anti-CD206 (Anti-CD206; 1:1000, 24,595,

(11) Rebalancing NTRK2 isoforms promotes vascular regeneration in bronchopulmonary dysplasiaCell Stem CellJanuary 8, 2026Yunpei Zhang, Cheng Tan, Ziyi Liu, Xiangdi Mao, Cheng Jiang, Afzaal Nadeem Mohammed, Xiaolei Li, Renzhong Lu, Anmin Wang, Wusiman Maihemuti, et al.,Cell Signaling,Cat#: 2118S Rabbit polyclonal to Ki67,Abcam,Cat#: ab15580 Sheep anti human CD31,Bio-Techne,Cat#: AF806 p44/42 MAPK (Erk1/2) Antibody,Cell signaling,Cat#: 9102S Phospho-p44/42 MAPK (Erk1/2) Antibody,Cell signaling

(12) Cooperative ETS transcription factors are required for lymphatic endothelial cell integrity and resilienceJ Clin InvestDecember 23, 2025Myung Jin Yang, Seok Kang, Seon Pyo Hong, Hokyung Jin, Jin-Hui Yoon, Cheolhwa Jin, Chae Min Yuk, Lydia Getachew Gebeyehu, Junho Jung, Sung-Hwan Yoon, et al.anti-VEGFR3/Flt4 (MAB3491); sheep anti-FoxC2 (AF5044); goat anti-VEGFR2/Flk-1 (AF357); sheep anti-CD31 (AF806); goat anti-GATA2 (AF2046) (R&D Systems); mouse anti-NFATC1 (MA3-024, Invitrogen); and rabbit anti-GAPDH

(13) Flow-driven construction of capillary-scale vessels with predefined geometries in natural hydrogelsMaterials Today BioDecember 1, 2025Akira Ono, Ryohei Ouchi, Yusei Kumagai, Masahiko Suda, Tadahiro Yamashita, Yoshihiro Taguchi, Ryo Sudocells were incubated overnight at 4 °C with primary antibodies: sheep anti-PECAM-1 (1:100 dilution; AF806, R&D Systems) and mouse anti-vimentin (1:200 dilution, SC6260; Santa Cruz). Subsequently, the samples

(14) Vessel‐associated microglia are differentially activated and distributed in relation to systemic infection and Alzheimer's diseaseBrain PathologyNovember 30, 2025Oliver Milner, Robert A. Fisher, Daniel J. Asby, Stephen Cross, Delphine Boche, J. Scott Minersovernight at 4°C at the following dilutions of stock concentration (0.2 mg/mL): CD31 (1:150, R&D Systems, AF806); CD68 (1:75, Dako, M0876); HLA‐DR (1:500, Abcam, AB20181); and IBA1 (1:1000, AlphaLabs Wako, 019‐19741).

(15) Modeling the metastatic tumor microenvironment: a co-culture platform of lung cancer and blood vessel organoids for drug evaluationScientific ReportsNovember 24, 2025Mingyun Lee, Yeo-Joon Yoon, Ji Yeon Lee, Mina Hwang, Sewon Park, Min Hak Lee, Sang-Won Um, Byoung Chul Cho, Jinguen Rheey, Toshiro Sato, et al.incubated for 3days at 4 °C. The following primary antibodies were used in this study: CD31 (R&D systems, AF806), PDGFRβ (Cell signaling, 3169 S, Danvers, MA, USA; R&D systems, AF385, Minneapolis, MN, USA), Fibronectin

(16) Modeling the Blood-Brain Barrier Using Human-Induced Pluripotent Stem CellsMethods in Molecular BiologyNovember 21, 2025Louise A. Mesentier-Louro, Natalie Suhy, Diede Broekaart, Michael Bula, Ana C. Pereira, Joel W. BlanchardCat. no. S2532–100 uL).Pecam1 (Platelet endothelial cell adhesion molecule 1, R & D Systems Cat. no. AF806).PDGFRβ (Platelet-derived growth factor receptor beta, R & D Biosystems, Cat. no. AF385).VE-cadherin

(17) Multi-cellular phenotypic dynamics during the progression of an immunocompetent breast cancer modeliScienceNovember 21, 2025Gsell L, Watson SS, Sutevski I, Massara M, Soukup K, Eroglu A, Mold JE, Cougnoux A, Joyce JA, Hausser J.anti-CD45-PE555,ThermoFisher Scientific,Cat #12-0461-82 Polyclonal goat anti-CD31,R&D System,Cat #AF806; RRID: AB_355617 Monoclonal Rat anti-LY6C (+LY6G),ThermoFisher Scientific,Cat #14-5931-82 Polyclonal

(18) Spatially patterned kidney assembloids recapitulate progenitor self-assembly and enable high-fidelity in vivo disease modelingCell Stem CellOctober 2, 2025Biao Huang, Pedro Medina, Jincan He, Zipeng Zeng, Sunghyun Kim, Janet Romo, Kari Koppitch, Chennan C. Zhang, Georgina Gyarmati, Yohan Park, et al.,Thermo Fisher Scientific,Cat# 14-4801; RRID: AB_2314387 Sheep Polyclonal anti-Human CD31,R&D Systems,Cat# AF806; RRID: AB_355617 Rat Monoclonal anti-Mouse CD31,BD Biosciences,Cat# 550274; RRID: AB_393571 Mouse

(19) Clenbuterol induces lean mass and muscle protein accretion, but attenuates cardiorespiratory fitness and desensitizes muscle β2‐adrenergic signallingThe Journal of PhysiologyOctober 1, 2025Morten Hostrup, Lukas Moesgaard, Mads Fischer, Kate Aiko Wickham, Mads Pleshardt, Andreas Breenfeldt Andersen, Jacob Bejder, Martin Thomassen, Jens J. Nielsen, Yvette Dehnes, et al.band intensity adjusted for background intensity. The primary antibodies used were: CD31/PECAM‐1 (#AF806; R&D Systems Inc., Minneapolis, MN, USA), OXPHOS (ab110411; Abcam, Cambridge, UK), phospho‐(Ser/Thr)

(20) Engineered vasculature induces functional maturation of pluripotent stem cell-derived islet organoidsDevelopmental CellSeptember 22, 2025Yesl Jun, Kim-Vy Nguyen-Ngoc, Somesh Sai, R. Hugh F. Bender, Winnie Gong, Vira Kravets, Han Zhu, Christopher J. Hatch, Michael Schlichting, Roberto Gaetani, et al.,Santa Cruz,Cat# SC7819 Mouse anti-glucagon,Sigma-Aldrich,Cat# G2654 Sheep anti-CD31 (human),R&D Systems,Cat# AF806 Rat anti-CD31 (mouse),Novus,Cat# NB100–1642 Rabbit anti-laminin,Sigma-Aldrich,Cat# L9393 Mouse anti-laminin

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