Mouse VEGFR2/KDR/Flk-1 Antibody AF644 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse VEGFR2/KDR/Flk-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF644
Quantity100 ug (also 25 ug)
ApplicationsBioassay,ELISA Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,CyTOF-ready,Dual RNAscope ISH-IHC Compatible
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ConcentrationLYOPH
PurityAntigen Affinity-purified
TargetVEGFR2/KDR/Flk-1
IsotypeIgG
ReactivityCanine,Human,Mouse,Rat,Transgenic Mouse
HostGoat
Antibody TypePrimary Antibodies
NCBI Full Gene Namekinase insert domain protein receptor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse VEGFR2, Ala20-Glu762, Accession # P35918
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesFlk1, orv, Flk-1, VEGFR2, 6130401C07, VEGFR-2, sVEGFR-2, Ly73, Krd-1

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for studying neural stem cells in the adult hippocampus?

Yes, the VEGFR2/KDR/Flk-1 antibody has been shown to be effective in studies involving neural stem cells. For instance, it was used to demonstrate that VEGF signaling is necessary for maintaining the proximity of adult neural stem cells to blood vessels in the hippocampus, which is crucial for their motility and adhesion (Dause et al., 2024).

Is this antibody suitable for use in immunohistochemistry for cardiac tissue?

The VEGFR2/KDR/Flk-1 antibody has been utilized in immunohistochemistry to study cardiac tissue, specifically in research examining the developmental origins of pathological conditions such as ventricular non-compaction cardiomyopathy (Choquet et al., 2018). It was employed to visualize VEGFR2 expression in the myocardium.

What species can this antibody detect, and is it specific?

This antibody detects VEGFR2 in multiple species, including human, mouse, rat, and canine. It has been shown to specifically recognize mouse VEGFR2 with approximately 10% cross-reactivity with recombinant human VEGFR2, and minimal cross-reactivity with other VEGF receptors (less than 2% with VEGFR1 and VEGFR3) (R&D Systems).

Has this antibody been cited in studies related to angiogenesis?

Yes, the VEGFR2/KDR/Flk-1 antibody has been cited in various studies investigating angiogenesis. For example, it was used to assess the role of VEGFR2 in the angiogenic responses triggered by 2-deoxyribose-1-phosphate, highlighting its importance in endothelial cell signaling and vascular development (Vara et al., 2018).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) VEGFR2 is required for VEGF-C-VEGFR3-PI3Kα-mediated sprouting lymphangiogenesisNat CommunMay 15, 2026Hans Schoofs, Yan Zhang, Henrik Ortsäter, Mariya Lytvyn, Rui Benedito, Taija Mäkinenantibodies: goat anti-mouse VEGFR3 (1:100) (#AF743, R&D Systems), goat anti-mouse VEGFR2 (1:100) (#AF644, R&D Systems), rat anti-mouse LYVE1 (1:200) (#MAB2125, R&D systems), rabbit anti-mouse LYVE1 (1:500)

(2) MYCT1-IFITM2/3 interaction links endothelial endolysosomal trafficking to white adipose tissue expansionJ Exp MedMay 4, 2026Laureline Wetterwald, Anna Köck, Tania Wyss, Silvia Arroz-Madeira, Borja Prat-Luri, Muriel Jaquet, Benoît Petit, Marie-Catherine Vozenin, Seppo Ylä-Herttuala, Valérie Dutoit, et al.anti-β-catenin,BD Biosciences,"Cat# 610154, RRID:AB_397555" Goat polyclonal anti-VEGFR2,R&D Systems,"Cat# AF644, RRID:AB_355500" Rabbit polyclonal anti-β-catenin,Sigma-Aldrich,"Cat# 06-734, RRID:AB_310231" Rabbit

(3) Brain-Derived Cystathionine β-Synthase-Generated H2S Attenuates Cerebral Ischemia-Reperfusion Injury via VEGFR2-Mediated Angiogenesis in MCAO/R RatsCurr Issues Mol BiolApril 18, 2026Shuai Liang, La Jiang, Yu Jiang, Shan Wang, Jia-Rong Jiang, Ji-Yue Wen, Zhi-Wu Chen, Shuo ChenChina); DAPI mounting medium (P0131, Beyotime, Shanghai, China); anti-CD31 (AF3628) and anti-VEGFR2 (AF644, R&D Systems, Minneapolis, MN, USA); anti-CBS (DF6410), ZO-1 (AF5145) and Claudin 5 (AF5216, Affinity Biosciences

(4) Selective Targeting of Tip Endothelial Cells as a Therapeutic Strategy for Tumor AngiogenesisAdvanced ScienceMarch 1, 2026Byoungmo Kim, Ha Kyeong Lee, Zulfikar Azam, Jeong Uk Choi, Riajul Wahab, Na Kyeong Lee, Yoon Gun Ko, So‐Young Choi, Se‐Ra Lee, Wan Seob Shim, et al.BD Biosciences), respectively. For VEGFR2 staining, goat anti‐mouse VEGFR2 polyclonal antibody (1:200, AF644, R&D Systems) followed by Alexa 488‐linked donkey anti‐goat antibodies were used. As a control, few

(5) Compartmentalized VEGF receptor expression in hypothalamic tanycytes reveals a novel non-endothelial axis of VEGF signaling : Tanycytes as a novel non-endothelial target of VEGF signalingFluids and Barriers of the CNSFebruary 14, 2026Ombeline Desruelle, Manon Leclerc, Sreekala Nampoothiri, Daniela Fernandois, Claude-Alain Maurage, Markus Schwaninger, Vincent Prevot, Ines Martinez-CorralIV,[1:500],AB769,Abcam Goat anti-mVEGF R1 (Flt-1),[1:100],AF471,R&D System Goat anti-mVEGF R2 (Flk-1),[1:50],AF644,R&D System Donkey anti-rabbit (488),[1:300],A21206,Invitrogen Donkey anti-goat (488),[1:300],A11055,Invitrogen Donkey

(6) Extraocular delivery of bioswitchable tri-miR-22-loaded tetrahedral DNA nanostructures for intraocular neovascular and neurodegenerative repairSignal Transduct Target TherFebruary 10, 2026Qiong Wang, You Wang, Li Chen, Junyang Huang, Tao Cai, Yi Lin, Jingying Liu, Jinnan Liu, Jiang Zhu, Rong Li, et al.Thermo Fisher Scientific, Massachusetts, USA), CD31(SC-376764, Santa Cruz Biotechnology, Dallas, TX, USA), VEGFR (#AF644; R&D Systems, Minneapolis, MN, USA), Tuj1 (#4466; Cell Signaling Technology, Danvers, MA, USA), GFAP

(7) Deficient chaperone-mediated autophagy in macrophages aggravates colitis and colitis-associated tumorigenesis in miceMolecules and CellsDecember 1, 2025Weichun Zhu, Zehao Chen, Yunqian Gao, Chungang Zhai, Xia Li, Ning Wang, Kang Fu, Wentao Chen, Jieqiong Peng, Dan Xu, et al.RI-neutralizing antibody (5 µg/mL, AF-401-NA, R&D Systems) and/or VEGFR2-neutralizing antibody (0.5 µg/mL, AF644, R&D Systems), respectively, for 24 hours. Hematoxylin and Eosin (H&E) Staining Colitis and CRC tissues

(8) Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down SyndromeImmunologyNovember 27, 2025Koki Harada, Keiichi Ishihara, Sayaka Wakayama, Teruhiko Wakayama, Sae Yamamoto, Haruhiko Sago, Shun Shimohama, Satoshi Akiba, Florent Ginhoux, Kazuyuki Takata(1:300, R&D Systems, MAB5051), anti‐VEGFR1 (1:8, R&D Systems, AF471), anti‐VEGFR2 (1:8, R&D Systems, AF644), anti‐VEGFR3 (1:80, R&D Systems, AF743), Mouse IgG2B Isotype Control (1:200, R&D Systems, MAB004) and

(9) Hypoxia regulate developmental coronary angiogenesis potentially through VEGF‐R2‐ and SOX17‐mediated signalingDevelopmental DynamicsFebruary 1, 2025Halie E. Vitali, Bryce Kuschel, Chhiring Sherpa, Brendan W. Jones, Nisha Jacob, Syeda A. Madiha, Sam Elliott, Eddie Dziennik, Lily Kreun, Cora Conatser, et al.Erg 1/2/3 (1:1000, Cat: ab92513, Abcam), an endothelial cell nucleus antibody; VEGF-R2 (1:100 Cat: AF644, R&D Systems); VE-Cadherin (1:125, Cat:550548, BD Pharmingen), an endothelial cell membrane marker;

(10) Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injectionSTAR ProtocJanuary 15, 2025Yalan Wu, Chenguang Wang, Xiao Yu Tian,Thermo Fisher Scientific,"Catalog # 65-6140, RRID: AB_2533969" Mouse VEGFR2/KDR/Flk-1 antibody (1: 200),R&D Systems,"Catalog # AF644, RRID:AB_355500" Anti-CD31 antibody (1: 200),Abcam,"Catalog # ab28364, RRID:AB_726362" Anti-alpha

(11) A Pax3 lineage gives rise to transient haematopoietic progenitorsDevelopmentDecember 1, 2024Giovanni Canu, Rosamaria Correra, Guillermo Diez-Pinel, Raphaël F. P. Castellan, Laura Denti, Alessandro Fantin, Christiana Ruhrbergstaining with the following antibodies as indicated in the figures: goat anti-mouse KDR (R&D Systems, #AF644, Lot COA0420091, 1:50), rat anti-mouse CD45 (BD Biosciences, #550539, Lot 9301732, 1:50), rabbit anti-mouse

(12) Specialized post-arterial capillaries facilitate adult bone remodellingNature cell biologyDecember 1, 2024Mohanakrishnan V, Sivaraj KK, Jeong HW, Bovay E, Dharmalingam B, Bixel MG, Dinh VV, Petkova M, Paredes Ugarte I, Kuo YT, et al.

(13) Development of pial collaterals by extension of pre-existing artery tipsCell ReportsOctober 22, 2024Suraj Kumar, Swarnadip Ghosh, Niloufer Shanavas, Vinayak Sivaramakrishnan, Manish Dwari, Soumyashree Dasdilution), Dach1 (Catalog number: 10194-1-AP, Proteintech, 1:500 dilution), VegfR2 (Catalog number: AF644, R&D Systems, 1:250 dilution), Endomucin (Catalog number: 14-5851-82; Thermo Fisher Scientific, 1:500

(14) Helminth infection driven gastrointestinal hypermotility is independent of eosinophils and mediated by alterations in smooth muscle instead of enteric neuronsPLoS pathogensAugust 1, 2024Wang H, Barry K, Zaini A, Coakley G, Moyat M, Daunt CP, Wickramasinghe LC, Azzoni R, Chatzis R, Yumnam B, et al.(AB1376) PGP9.5,Mouse,1:100,Abcam (ab8189) SiglecF,Rat,1:100,BD biosciences (552125) VEGFR2,Goat,1:100,R&D Systems (AF644) VIP,Rabbit,1:500,Immunostar (20077) Secondary antibodies,Secondary antibodies,Secondary antibodies,Secondary

(15) Autocrine VEGF drives neural stem cell proximity to the adult hippocampus vascular nicheLife Science AllianceJuly 1, 2024Tyler J Dause, Jiyeon K Denninger, Robert Osap, Ashley E Walters, Joshua D Rieskamp, Elizabeth D Kirby1:100)",Millipore,Cat#MAB353 RRID: AB_94911 "Goat anti-VEGFR2 (IF in vivo, 1:100)",R&D Systems,Cat#AF644 RRID: AB_355500 Jackson Immuno Research Labs Biotin-SP (long spacer) AffiniPure Donkey Anti-Rabbit IgG (H+L

(16) SMAD4 : A critical regulator of cardiac neural crest cell fate and vascular smooth muscle developmentDevelopmental DynamicsJanuary 1, 2024Brianna E. Alexander, Huaning Zhao, Sophie Astrofab167453), Mouse anti-Tuj1 (1:150, Covance, MMS-435P), and Goat anti-VEGFR2 (1:200, R&D Systems Inc., AF644). Primary antibodies were detected with secondary antibodies from Invitrogen (1:300): donkey anti-rabbit

(17) Angiopoietin-2 blockade suppresses growth of liver metastases from pancreatic neuroendocrine tumors by promoting T cell recruitmentThe Journal of clinical investigationOctober 16, 2023Lee E, O'Keefe S, Leong A, Park HR, Varadarajan J, Chowdhury S, Hiner S, Kim S, Shiva A, Friedman RA, et al.temperature with the following primary antibodies: SV40 T antigen (Santa Cruz, SC20800), VEGFR2 (R&D Systems, AF644), CD31 (Thermo Fisher Scientific, MA3105), TIE2 (Regeneron Pharmaceuticals Inc., human monoclonal, REGN1376),

(18) Sox7-positive endothelial progenitors establish coronary arteries and govern ventricular compactionEMBO reportsOctober 9, 2023Chiang IK, Humphrey D, Mills RJ, Kaltzis P, Pachauri S, Graus M, Saha D, Wu Z, Young P, Sim CB, et al.Sigma Aldrich), anti‐V5 (1:200, AB3792, Merck), anti‐TPM (1:500; T9283, Sigma), anti‐VEGFR2 (1:200, AF644, R&D System), anti‐CX40 (1:300, CX‐40A, Alpha Bio‐diagnostic), anti‐SMA (1:200, 14395‐1‐AP, Proteintech),

(19) Dedifferentiation and Proliferation of Artery Endothelial Cells Drive Coronary Collateral Development in MiceArteriosclerosis, thrombosis, and vascular biologyAugust 1, 2023Arolkar G, Kumar SK, Wang H, Gonzalez KM, Kumar S, Bishnoi B, Rios Coronado PE, Woo YJ, Red-Horse K, Das S.catalog number CX40-A, RRID: AB_1616128; Alpha Diagnostics Int, Inc), Anti-VEGFR2 (1:125, catalog number AF644, RRID: AB_355500; R&D Systems). Secondary antibodies: Alexa fluor-conjugated antibodies (488, 555, and

(20) VEGFR3 is required for button junction formation in lymphatic vesselsCell reportsJuly 25, 2023Jannaway M, Iyer D, Mastrogiacomo DM, Li K, Sung DC, Yang Y, Kahn ML, Scallan JP.,Life Technologies,A21311; RRID:AB_221477 goat anti-VEGFR3,R&D Systems,AF743; RRID:AB_355563 goat anti-VEGFR2,R&D Systems,AF644; RRID:AB_355500 rabbit anti-VEGFR2,ThermoFisher Scientific,MA5–15157; RRID:AB_10986085 rabbit anti-LYVE1,AngioBio,11–034;

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VEGFR2/KDR/Flk-1 Antibody

VEGFR2/KDR/Flk-1 Antibody

VEGFR2/KDR/Flk-1 Antibody

VEGFR2/KDR/Flk-1 Antibody

VEGFR2/KDR/Flk-1 Antibody

Mouse VEGFR2/KDR/Flk-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (122)

Reviews

Mouse VEGFR2/KDR/Flk-1 Antibody from R&D Systems, a Bio-Techne Brand

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