Mouse M-CSF Antibody AF416 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse M-CSF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF416
ApplicationsWestern Blot,Flow Cytometry,Immunohistochemistry,Western Blot,In vivo assay,Neutralization,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
HostGoat
IsotypeIgG
ReactivityMouse,Rat
TargetM-CSF
Conjugate/TagUnconjugated
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse M-CSF, Lys33-Glu262, Accession # Q3U4F9
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecolony stimulating factor 1 (macrophage)
NCBI Gene AliasesBAP025, Csfm, MCSF, Mhdabap25, PG-M-CSF, op

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Systemic CSF1R Targeting Depletes Pathogenic MPS Bubs and Ameliorates Psoriasis via PPARα-mediated ResolutionTheranosticsJanuary 30, 2026Zhen-Jia Lin, Ying Li, Yangyinhui Yu, Mei-Jia Fang, Ying Xiong, Rui Xu, Zhuo Wang, Jun Zhang, Ya-Nan Xu, Jun-Ya Wan, et al.serum-TBS. IF staining was performed by using rabbit anti-CSF1R (sc-692, Santa Cruz; 1:500), goat-anti-CSF1 (AF416, R&D System; 1:400), ribbit anti-Ki67 (ab16667, Abcam; 1:500), rat anti-F4/80 (123102, BioLegend, 1:100),

(2) Dorsal root ganglia CSF1 + neuronal subtypes have different impact on macrophages and microglia after spared nerve injuryJournal of the Peripheral Nervous SystemDecember 1, 2024Andreea Violeta Grosu, Roxana‐Olimpia Gheorghe, Alexandru Filippi, Alexandru Florian Deftu, Manon Isler, Marc Suter, Violeta RistoiuLouis, MO, USA), CGRP (1:1000, rabbit, T4032, BMA Biomedicals, Augst, Switzerland), CSF1 (1:300, goat, AF416, R&D Systems, Minneapolis, MN, USA), and biotinylated isolectin B4 (IB4) (1:100, B‐1205, Vector laboratories,

(3) The choroid plexus synergizes with immune cells during neuroinflammationCellSeptember 5, 2024Xu H, Lotfy P, Gelb S, Pragana A, Hehnly C, Byer LIJ, Shipley FB, Zawadzki ME, Cui J, Deng L, et al.anti-GFP,Abcam,Cat# ab13970; AB_300798 F4/80-PECy7,BioLegend,Cat# 123113; AB_893490 Goat anti-MCSF,R&D Systems,Cat# AF416; AB_355351 Goat anti-S100A9,R&D Systems,Cat# AF2065; AB_2184263 Goat anti-type IV Collagen-Alexa Fluor

(4) Sclerostin blockade inhibits bone resorption through PDGF receptor signaling in osteoblast lineage cellsJCI InsightMay 22, 2024Cyril Thouverey, Pierre Apostolides, Julia Brun, Joseph Caverzasio, Serge Ferrarimurine sclerostin (Peprotech), with or without 1.25 μg/mL Scl-Ab or 500 ng/mL anti–M-CSF antibody (AF416, R&D Systems). After 8 days, cocultures were fixed and stained, and multinucleated TRAcP-positive cells

(5) Modulation of central synapse remodeling after remote peripheral injuries by the CCL2-CCR2 axis and microgliaCell reportsFebruary 27, 2024Rotterman TM, Haley-Johnson Z, Pottorf TS, Chopra T, Chang E, Zhang S, McCallum WM, Fisher S, Franklin H, Alvarez M, et al.anti-ATF3Clone CL1685,Novus Biologicals,Cat# NBP2-34489;Clone CL1685 Goat anti-CSF1,R&D Systems,Cat# AF416;RRID#: AB_355351 Rabbit anti-Iba1,Wako Chemical,Cat#019-19741; RRID#AB_839504 Chicken anti-Iba1,Synaptic Systems

(6) A collaboration between immune cells and the choroid plexus epithelium in brain inflammationbioRxiv : the preprint server for biologyAugust 8, 2023Xu H, Lotfy P, Gelb S, Pragana A, Hehnly C, Shipley FB, Zawadzki ME, Cui J, Deng L, Taylor M, et al.

(7) Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic painProg NeurobiolMarch 1, 2023Tassou A, Thouaye M, Gilabert D, Jouvenel A, Leyris JP, Sonrier C, Diouloufet L, Mechaly I, Mallié S, Bertin J, et al.

(8) Diet-induced inflammation in the anterior paraventricular thalamus induces compulsive sucrose-seekingNature neuroscienceAugust 1, 2022Cheng J, Ma X, Li C, Ullah R, Wang X, Long J, Yuan Z, Liu S, Fu J, Chen Z, et al.

(9) Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineagesJournal of neuroinflammationJuly 19, 2021De I, Maklakova V, Litscher S, Boyd MM, Klemm LC, Wang Z, Kendziorski C, Collier LS.,Millipore Sigma,1:150,AB_570666, GFAP,Chicken,ab4674,Abcam,1:200,AB_304558, GFAP,Rabbit,ab7260,Abcam,1:100,AB_305808, CSF1,Goat,AF416,R&D systems,1:25,AB_355351, PDGFRA,Goat,AF1062,R&D systems,1:200,AB_2236897, Secondary antibodies,Secondary

(10) Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp MacrophagesImmunityJuly 14, 2020Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart BJ, Brouilly N, Malissen B, Clatworthy MR, Bajénoff M.(2B8) Biolegend Cat#108114; RRIDAB_493596 Anti-mouse CSF-1 (Polyclonal Goat IgG) R&D Systems Cat#AF416; RRID: AB_355351 Anti-mouse Desmin (Polyclonal Rabbit IgG) Monosan Cat#PS031; RRID: AB_420229 Anti-mouse

(11) Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage NicheImmunityOctober 15, 2019Bonnardel J, T'Jonck W, Gaublomme D, Browaeys R, Scott CL, Martens L, Vanneste B, De Prijck S, Nedospasov SA, Kremer A, et al.,Thermo Fisher,Cat#50-0443-82; RRID: AB_10597449 "Anti-mouse M-CSF Polyclonal antibody (Goat), unconjugated",R&D Systems,Cat#AF416; RRID: AB_355351 "Anti-mouse Siglec F Monoclonal antibody (Rat, clone E50-2440) BUV395 conjugated",BD Biosciences

(12) Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic PainCell reportsJune 25, 2019Zhou LJ, Peng J, Xu YN, Zeng WJ, Zhang J, Wei X, Mai CL, Lin ZJ, Liu Y, Murugan M, et al.AB5060, Millipore, USA.), rabbit anti-Iba1 (1:2000, 019–19741, Wako), goat anti-M-CSF antibody (1:500, AF416, R&D Systems, USA); rat anti-CD11b (1:500, 101202, Biolegend, USA); goat anti-Iba1 (1:500, ab5076, Abcam,

(13) Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage NicheImmunityJune 18, 2019Mondor I, Baratin M, Lagueyrie M, Saro L, Henri S, Gentek R, Suerinck D, Kastenmuller W, Jiang JX, Bajénoff M.(Polyclonal Rabbit IgG),Acris Antibodies,DP3513P Anti-mouse CSF-1 (Polyclonal Goat IgG),R&D Systems,AF416 Anti-mouse IBA1,Antibodies-online,ABIN2857032 "Anti-GFP polyclonal antibody, AF488",Life Technologies,A21311 Anti-RFP,Antibodies-online,ABIN1043867 Anti-rabbit

(14) Spinal Motor Circuit Synaptic Plasticity after Peripheral Nerve Injury Depends on Microglia Activation and a CCR2 MechanismThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 1, 2019Rotterman TM, Akhter ET, Lane AR, MacPherson KP, García VV, Tansey MG, Alvarez FJ.Synaptic Systems catalog #131011 Wako catalog #019-19741 BD Biosciences catalog #553238 R&D Systems catalog #AF416 EMD Millipore catalog #MAB377 AB_620519 AB_10013483 AB_887878 AB_887875 AB_887883 AB_887872 AB_839504 AB_394727 AB_355351 AB_2298772 1:1000 1:1000 1:1000 1:500 1:500 1:200 1:500 1:100 1:2000 1:500 retrograde

(15) Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal lossGliaOctober 1, 2018Gushchina S, Pryce G, Yip PK, Wu D, Pallier P, Giovannoni G, Baker D, Bo X.for immunohistochemistry Molecules Host species Sources Catalogue numbers RRID Dilution Mouse CSF1 Goat PeproTech 500-P62G - 1:200 Mouse CSF1 Goat R&D Systems AF416 AB_355351 1:200 GFAP Rabbit Dako Z0334 AB_10013382 1:500 NeuN Rabbit Abcam ab177487 AB_2532109 1:200 Iba1 Rabbit Wako 019-19741 AB_839504 1:400 Iba1 Goat Novus NB100-1028 AB_521594 1:400 TMEM119 Rabbit Abcam Ab185333 AB_2687894 1:200 MAG Rabbit Thermo Fisher

(16) Mice lacking Kcns1 in peripheral neurons show increased basal and neuropathic pain sensitivityPainAugust 1, 2018Tsantoulas C, Denk F, Signore M, Nassar MA, Futai K, McMahon SB.Merck Millipore AB5539), mouse anti-Nav1.8 (1:1000, Neuromab N134/12), goat anti-CSF1 (1:1000, R&D Systems #AF416), and rabbit anti-ATF3 (1:500, Santa Cruz Biotechnology #sc-188). Secondary antibodies used were donkey

(17) Microglial pannexin-1 channel activation is a spinal determinant of joint painScience advancesAugust 1, 2018Mousseau M, Burma NE, Lee KY, Leduc-Pessah H, Kwok CHT, Reid AR, O'Brien M, Sagalajev B, Stratton JA, Patrick N, et al.Gels were transferred onto nitrocellulose membrane and probed with goat anti-Csf1 antibody (1:1000, AF416, R&D Systems) and mouse anti–β-actin (1:2000, A5316, Sigma). Membranes were washed in TBST (20 mM tris,

(18) Inhibition of neuronal FLT3 receptor tyrosine kinase alleviates peripheral neuropathic pain in miceNature communicationsMarch 12, 2018Rivat C, Sar C, Mechaly I, Leyris JP, Diouloufet L, Sonrier C, Philipson Y, Lucas O, Mallié S, Jouvenel A, et al.1:500 in spinal cord or 1:1000 in DRGs), anti-IBA1 (Wako 019-19741, rabbit 1:500), antiCSF1 (R&D System AF416, goat 1:500), anti-CD45 (Millipore 05-1416, rat 1:100), anti-CD11b (DSHB M1/70.15.11.5.2, rat 1:100)

(19) Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2αNature communicationsFebruary 8, 2018Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, Duan H, Ma P, Zhang L, Zamvil SS, et al.FBS. Cells were treated with specific neutralizing antibodies against CSF-1 (1 μg/ml, R&D Systems, AF416) and IL-6 (1 μg/ml, BioLegend, 504505), or control IgG (Sigma). Cells were fixed and subjected to immunofluorescence analysis

(20) Lymphatic vessels regulate immune microenvironments in human and murine melanomaThe Journal of clinical investigationSeptember 1, 2016Lund AW, Wagner M, Fankhauser M, Steinskog ES, Broggi MA, Spranger S, Gajewski TF, Alitalo K, Eikesdal HP, Wiig H, et al.

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M-CSF Antibody

M-CSF Antibody

Mouse M-CSF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (22)

Reviews

Mouse M-CSF Antibody from R&D Systems, a Bio-Techne Brand

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