M-CSF Antibody AF416 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemM-CSF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF416
ApplicationsBioassay,Western Blot,Flow Cytometry,Immunohistochemistry,Western Blot,In vivo assay,Neutralization,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
HostGoat
IsotypeIgG
ReactivityHuman,Mouse,Rat
TargetM-CSF
Conjugate/TagUnconjugated
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse M-CSF, Lys33-Glu262, Accession # Q3U4F9
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namecolony stimulating factor 1
NCBI Gene AliasesCSF-1, MCSF, PG-M-CSF

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human M-CSF is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Diabetes reprograms brain and systemic immune landscapes to impair repair after injuryMol BrainJune 9, 2026Keon Ah Lee, Su Yeon Kim, Jin Soo Lee, Haijie Yang, Jiawei An, Sang Myun Park, Eun Jeong Lee, Eun-Hye Joeantibodies (Synaptic Systems, cat. no. 173006; 1:1000), mouse anti-M-CSF antibodies (R&D Systems, cat. no. AF416; 1:500), Goat anti-ARG antibodies (Abcam, cat. no. ab60176; 1:500), Guinea pig anti-MAP2 antibodies

(2) Expression and Role of Colony Stimulating Factor 1 Receptor During OdontogenesisJ Dev BiolMay 18, 2026Ashina Nagra, Ling-Yi Chen, Soheil Saeidiborojeni, Jessica M. Rosin, Siddharth R. VoraMinneapolis, MN, USA; Cat# AF3818; RRID:AB_884158) with or without goat anti-mouse CSF1 (10 μg/mL, R&D Cat # AF416, RRID:AB_355351). Negative controls used pre-immune sheep IgG and/or goat IgG at matched concentrations.

(3) Systemic CSF1R Targeting Depletes Pathogenic MPS Bubs and Ameliorates Psoriasis via PPARα-mediated ResolutionTheranosticsJanuary 30, 2026Zhen-Jia Lin, Ying Li, Yangyinhui Yu, Mei-Jia Fang, Ying Xiong, Rui Xu, Zhuo Wang, Jun Zhang, Ya-Nan Xu, Jun-Ya Wan, et al.serum-TBS. IF staining was performed by using rabbit anti-CSF1R (sc-692, Santa Cruz; 1:500), goat-anti-CSF1 (AF416, R&D System; 1:400), ribbit anti-Ki67 (ab16667, Abcam; 1:500), rat anti-F4/80 (123102, BioLegend, 1:100),

(4) Fluid-Niche and Microglial Signatures Prime Robust Intraventricular Macrophage Response to Blood During Brain DevelopmentbioRxiv : the preprint server for biologyOctober 2, 2025Zawadzki ME, Hehnly C, Benson JC, Figueroa Velez DX, Gelb S, Stanley C, Byer LIJ, Xu H, Sadegh C, Pragana A, et al.

(5) Dorsal root ganglia CSF1 + neuronal subtypes have different impact on macrophages and microglia after spared nerve injuryJournal of the Peripheral Nervous SystemDecember 1, 2024Andreea Violeta Grosu, Roxana‐Olimpia Gheorghe, Alexandru Filippi, Alexandru Florian Deftu, Manon Isler, Marc Suter, Violeta RistoiuLouis, MO, USA), CGRP (1:1000, rabbit, T4032, BMA Biomedicals, Augst, Switzerland), CSF1 (1:300, goat, AF416, R&D Systems, Minneapolis, MN, USA), and biotinylated isolectin B4 (IB4) (1:100, B‐1205, Vector laboratories,

(6) The choroid plexus synergizes with immune cells during neuroinflammationCellSeptember 5, 2024Xu H, Lotfy P, Gelb S, Pragana A, Hehnly C, Byer LIJ, Shipley FB, Zawadzki ME, Cui J, Deng L, et al.anti-GFP,Abcam,Cat# ab13970; AB_300798 F4/80-PECy7,BioLegend,Cat# 123113; AB_893490 Goat anti-MCSF,R&D Systems,Cat# AF416; AB_355351 Goat anti-S100A9,R&D Systems,Cat# AF2065; AB_2184263 Goat anti-type IV Collagen-Alexa Fluor

(7) Sclerostin blockade inhibits bone resorption through PDGF receptor signaling in osteoblast lineage cellsJCI InsightMay 22, 2024Cyril Thouverey, Pierre Apostolides, Julia Brun, Joseph Caverzasio, Serge Ferrarimurine sclerostin (Peprotech), with or without 1.25 μg/mL Scl-Ab or 500 ng/mL anti–M-CSF antibody (AF416, R&D Systems). After 8 days, cocultures were fixed and stained, and multinucleated TRAcP-positive cells

(8) A collaboration between immune cells and the choroid plexus epithelium in brain inflammationbioRxiv : the preprint server for biologyAugust 8, 2023Xu H, Lotfy P, Gelb S, Pragana A, Hehnly C, Shipley FB, Zawadzki ME, Cui J, Deng L, Taylor M, et al.

(9) Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic painProg NeurobiolMarch 1, 2023Tassou A, Thouaye M, Gilabert D, Jouvenel A, Leyris JP, Sonrier C, Diouloufet L, Mechaly I, Mallié S, Bertin J, et al.

(10) Diet-induced inflammation in the anterior paraventricular thalamus induces compulsive sucrose-seekingNature neuroscienceAugust 1, 2022Cheng J, Ma X, Li C, Ullah R, Wang X, Long J, Yuan Z, Liu S, Fu J, Chen Z, et al.

(11) Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineagesJournal of neuroinflammationJuly 19, 2021De I, Maklakova V, Litscher S, Boyd MM, Klemm LC, Wang Z, Kendziorski C, Collier LS.,Millipore Sigma,1:150,AB_570666, GFAP,Chicken,ab4674,Abcam,1:200,AB_304558, GFAP,Rabbit,ab7260,Abcam,1:100,AB_305808, CSF1,Goat,AF416,R&D systems,1:25,AB_355351, PDGFRA,Goat,AF1062,R&D systems,1:200,AB_2236897, Secondary antibodies,Secondary

(12) Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp MacrophagesImmunityJuly 14, 2020Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart BJ, Brouilly N, Malissen B, Clatworthy MR, Bajénoff M.(2B8) Biolegend Cat#108114; RRIDAB_493596 Anti-mouse CSF-1 (Polyclonal Goat IgG) R&D Systems Cat#AF416; RRID: AB_355351 Anti-mouse Desmin (Polyclonal Rabbit IgG) Monosan Cat#PS031; RRID: AB_420229 Anti-mouse

(13) Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage NicheImmunityOctober 15, 2019Bonnardel J, T'Jonck W, Gaublomme D, Browaeys R, Scott CL, Martens L, Vanneste B, De Prijck S, Nedospasov SA, Kremer A, et al.,Thermo Fisher,Cat#50-0443-82; RRID: AB_10597449 "Anti-mouse M-CSF Polyclonal antibody (Goat), unconjugated",R&D Systems,Cat#AF416; RRID: AB_355351 "Anti-mouse Siglec F Monoclonal antibody (Rat, clone E50-2440) BUV395 conjugated",BD Biosciences

(14) Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic PainCell reportsJune 25, 2019Zhou LJ, Peng J, Xu YN, Zeng WJ, Zhang J, Wei X, Mai CL, Lin ZJ, Liu Y, Murugan M, et al.AB5060, Millipore, USA.), rabbit anti-Iba1 (1:2000, 019–19741, Wako), goat anti-M-CSF antibody (1:500, AF416, R&D Systems, USA); rat anti-CD11b (1:500, 101202, Biolegend, USA); goat anti-Iba1 (1:500, ab5076, Abcam,

(15) Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage NicheImmunityJune 18, 2019Mondor I, Baratin M, Lagueyrie M, Saro L, Henri S, Gentek R, Suerinck D, Kastenmuller W, Jiang JX, Bajénoff M.(Polyclonal Rabbit IgG),Acris Antibodies,DP3513P Anti-mouse CSF-1 (Polyclonal Goat IgG),R&D Systems,AF416 Anti-mouse IBA1,Antibodies-online,ABIN2857032 "Anti-GFP polyclonal antibody, AF488",Life Technologies,A21311 Anti-RFP,Antibodies-online,ABIN1043867 Anti-rabbit

(16) Spinal Motor Circuit Synaptic Plasticity after Peripheral Nerve Injury Depends on Microglia Activation and a CCR2 MechanismThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 1, 2019Rotterman TM, Akhter ET, Lane AR, MacPherson KP, García VV, Tansey MG, Alvarez FJ.Synaptic Systems catalog #131011 Wako catalog #019-19741 BD Biosciences catalog #553238 R&D Systems catalog #AF416 EMD Millipore catalog #MAB377 AB_620519 AB_10013483 AB_887878 AB_887875 AB_887883 AB_887872 AB_839504 AB_394727 AB_355351 AB_2298772 1:1000 1:1000 1:1000 1:500 1:500 1:200 1:500 1:100 1:2000 1:500 retrograde

(17) Mice lacking Kcns1 in peripheral neurons show increased basal and neuropathic pain sensitivityPainAugust 1, 2018Tsantoulas C, Denk F, Signore M, Nassar MA, Futai K, McMahon SB.Merck Millipore AB5539), mouse anti-Nav1.8 (1:1000, Neuromab N134/12), goat anti-CSF1 (1:1000, R&D Systems #AF416), and rabbit anti-ATF3 (1:500, Santa Cruz Biotechnology #sc-188). Secondary antibodies used were donkey

(18) Inhibition of neuronal FLT3 receptor tyrosine kinase alleviates peripheral neuropathic pain in miceNature communicationsMarch 12, 2018Rivat C, Sar C, Mechaly I, Leyris JP, Diouloufet L, Sonrier C, Philipson Y, Lucas O, Mallié S, Jouvenel A, et al.1:500 in spinal cord or 1:1000 in DRGs), anti-IBA1 (Wako 019-19741, rabbit 1:500), antiCSF1 (R&D System AF416, goat 1:500), anti-CD45 (Millipore 05-1416, rat 1:100), anti-CD11b (DSHB M1/70.15.11.5.2, rat 1:100)

(19) Lymphatic vessels regulate immune microenvironments in human and murine melanomaThe Journal of clinical investigationSeptember 1, 2016Lund AW, Wagner M, Fankhauser M, Steinskog ES, Broggi MA, Spranger S, Gajewski TF, Alitalo K, Eikesdal HP, Wiig H, et al.

(20) Both cell-surface and secreted CSF-1 expressed by tumor cells metastatic to bone can contribute to osteoclast activationExperimental Cell ResearchAugust 15, 2009Kader Yagiz, Susan R RittlingWestern blotting was as described previously [23]. CSF -1 was detected with biotinylated goat anti-CSF antibody (#AF416, R&D Systems, Minneapolis, MN); anti-beta-actin antibody (Sigma) was used for controls. CSF-1 levels

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M-CSF Antibody

M-CSF Antibody

M-CSF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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M-CSF Antibody from R&D Systems, a Bio-Techne Brand

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