AgRP/ART Antibody AF634 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemAgRP/ART Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF634
NCBI Gene AliasesArt, AGRT, ART, Agrt, ASIP2
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ReactivityMouse,Rat,Transgenic Mouse
HostGoat
TargetAgRP/ART
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameagouti related neuropeptide
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse AgRP/ART, Val25-Thr131, Accession # P56473
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse AgRP in direct ELISAs.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study AgRP expression in mouse models of obesity?

Yes, the AgRP/ART Antibody has been shown to detect Agouti-related peptide in mouse models, including studies that investigate obesity mechanisms. For instance, in a study involving Mecp2-null mice, the antibody was utilized to assess AgRP protein levels, which were found to be increased in high-fat diet conditions, indicating its relevance in obesity research ().

Is this antibody effective for immunohistochemistry applications in the hypothalamus?

The AgRP/ART Antibody has been successfully used in immunohistochemistry to visualize AgRP-expressing neurons in the hypothalamic arcuate nucleus. This application is crucial for understanding the role of AgRP in energy homeostasis and feeding behavior, as demonstrated in various studies (; ).

What is the recommended dilution for using this antibody in Western blotting?

The AgRP/ART Antibody is recommended to be used at a dilution of 1:3000 for Western blot applications, as indicated in the literature where it was employed to analyze AgRP protein levels in various experimental setups ().

Does this antibody have any known cross-reactivity with other species?

The AgRP/ART Antibody is specifically reactive with mouse and rat samples, making it suitable for studies involving these species. Its specificity has been confirmed in direct ELISA assays, ensuring reliable results in experiments focused on AgRP in these models.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Diet-Dependent Effects of Intracerebroventricular Maresin-1 on Hypothalamus of RatsInt J Mol SciSeptember 9, 2026Cihan Suleyman Erdogan, Irem Solmaz, Cansu Yakin, Fatma Ozge Tuncel, Tuba Keskin, Suat Tekin, Suleyman Sandal, Bayram Yilmaztemperature for 45 min. Primary antibodies against AgRP (4 μg/mL, R&D Systems, Minneapolis, MN, USA, AF634) and POMC (4 μg/mL, Novus Biologicals, Centennial, CO, USA, NB100-1533) were prepared in PBS with 1%

(2) Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cellsCell Stem CellJuly 2, 2026Zehra Abay-Nørgaard, Anika K. Mueller, Erno Hänninen, Dylan Rausch, Louise Piilgaard, Lucía Sena Trujillo, Lorenzo Fedrizzi, Jens Bager Christensen, Alison Salvador, Alrik L. Schörling, et al.(Rabbit),Phoenix Pharma,"Cat#H-003-53 (discontinued), RRID: AB_2313908" AGRP (Goat),R&D Systems,"Cat#AF634, RRID: AB_2273824" aMSH (Sheep),Millipore,"Cat#AB5087, RRID: AB_91683" bMSH (Rabbit,LSBio,Cat# LS-C183969-50 MAP2

(3) Tak1 licenses mitochondrial transfer from astrocytes to POMC neurons to maintain glucose and cholesterol homeostasisCell reportsDecember 24, 2024Yin K, Zhang T, Lu X, Shen Q, Gu K, Huang Y, Li C, Hou J, Li J, Zhang G.

(4) Regulation of hypothalamic reactive oxygen species and feeding behavior by phosphorylation of the beta 2 thyroid hormone receptor isoformSci RepMarch 26, 2024Svetlana Minakhina, Sun Young Kim, Fredric E. WondisfordGermany), rabbit anti-β-endorphin (1:200, Novus Biologicals/ Biotechne, MN, USA), goat anti-AgRP (1:200, AF634, R&D Systems, MN, USA). Conjugated antibody, anti-HA-Fluorescein (3F10 1:100, Roche, Diagnostics, Germany),

(5) Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in MiceNutrientsFebruary 21, 2023Decourtye-Espiard L, Clemessy M, Leneuve P, Mire E, Ledent T, Le Bouc Y, Kappeler L.H-003-57","Rabbit, polyclonal",1/500 AgRP #,Agouti-Related Protein (AGRP) (25–131) (Mouse) Antibody,"R&D systems, AF634","Goat, polyclonal",1/250 GFP,Anti-GFP antibody,"Abcam, ab6556","Rabbit, polyclonal",1/1000 GHRH #,Anti-GHRH

(6) Malocclusion impairs cognitive behavior via AgRP signaling in adolescent miceFrontiers in neuroscienceJanuary 1, 2023Kusumoto J, Ataka K, Iwai H, Oga Y, Yamagata K, Marutani K, Ishikawa T, Asakawa A, Miyawaki S.were verified with 10 μL of 0.05% cresyl violet dye. 2.10. Drug administration Anti-AgRP antibody (AF634; R&D Systems, Minneapolis, MN, USA) was dissolved in saline solution (0.9% NaCl) and administered intracerebroventricularly

(7) A subpopulation of agouti-related peptide neurons exciting corticotropin-releasing hormone axon terminals in median eminence led to hypothalamic-pituitary-adrenal axis activation in response to food restrictionFrontiers in molecular neuroscienceJanuary 1, 2022Yesmin R, Watanabe M, Sinha AS, Ishibashi M, Wang T, Fukuda A.(1:2000; Abcam, ab208942), chicken anti-GFP (1:1000; Abcam, ab13970), goat anti-AgRP (1:1000; R&D system, AF634), rabbit anti-TH (1:3000; Millipore, Ab152), mouse anti-HA (1:1000; Biolegend, 901501), rabbit anti-c-Fos

(8) Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With AgingFrontiers in aging neuroscienceJanuary 1, 2020Sugiura A, Shimizu T, Kameyama T, Maruo T, Kedashiro S, Miyata M, Mizutani K, Takai Y.USA), Iba1 (019-19741, Wako, Osaka, Japan), ACTH (sc-57021, B427, Santa Cruz Biotechnology), and AgRP (AF634, R&D Systems). Alexa Fluor 488-conjugated Sox2 Ab was obtained from Thermo Fisher Scientific (#53-9811-82,

(9) A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body MetabolismCell reportsMarch 12, 2019Ou Z, Ma Y, Sun Y, Zheng G, Wang S, Xing R, Chen X, Han Y, Wang J, Lu QR, et al.RRID: AB_2107436 POMC,Phoenix Pharmaceuticals,Cat# H-029–30; RRID:AB_2307442 AGRP,R&D Systems,Cat# AF634; RRID:AB_2273824 phosphorylated STAT3,Cell Signaling Technology,Cat# 9131; RRID:AB_331586 STAT3,Cell Signaling Technology

(10) Functional identity of hypothalamic melanocortin neurons depends on Tbx3Nature metabolismFebruary 1, 2019Quarta C, Fisette A, Xu Y, Colldén G, Legutko B, Tseng YT, Reim A, Wierer M, De Rosa MC, Klaus V, et al.were incubated with the following primary antibodies: rabbit anti-Pomc  goat anti-Agrp (R&D systems, AF634), precursor (Phoenix Pharmaceuticals),  chicken anti-GFP (Acris, AP31791PU-N), goat anti-GFP (Abcam,

(11) High-fat diet accelerates extreme obesity with hyperphagia in female heterozygous Mecp2-null micePloS oneJanuary 1, 2019Fukuhara S, Nakajima H, Sugimoto S, Kodo K, Shigehara K, Morimoto H, Tsuma Y, Moroto M, Mori J, Kosaka K, et al.Japan), MeCP2 (1:5000; #3456, Cell Signaling Technology Japan), Agouti-related peptide (AgRP; 1:3000, #AF634, R&D Systems, Minneapolis, MN, USA), proopiomelanocortin (POMC; 1:3000, #ab32893, Abcam, Cambridge,

(12) Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nucleiNeural developmentMay 21, 2016Sokolowski K, Tran T, Esumi S, Kamal Y, Oboti L, Lischinsky J, Goodrich M, Lam A, Carter M, Nakagawa Y, et al.anti-Pomc (1:100, ab322893, Abcam), goat anti-Pmch (1:1000, sc-14509, Santa Cruz), goat anti-Agrp (1:1000, AF634, R&D Systems), rabbit anti-c-Fos (1:1000, sc52, Santa Cruz), rabbit anti-Hcrt (1:1000, AB3096, Millipore),

(13) QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like BehaviorPloS oneJanuary 1, 2016Okamoto K, Yamasaki M, Takao K, Soya S, Iwasaki M, Sasaki K, Magoori K, Sakakibara I, Miyakawa T, Mieda M, et al.

(14) MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleusPloS oneJanuary 1, 2013Cortes-Campos C, Elizondo R, Carril C, Martínez F, Boric K, Nualart F, Garcia-Robles MA.

(15) Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balanceAutophagyJanuary 1, 0001"Kim S, Kim N, Park S, Jeon Y, Lee J, Yoo SJ, Lee JW, Moon C, Yu SW, Kim EK."Novus Biologicals; LAMP2 (L0668), MAP1LC3B/LC3B (L8918), SQSTM1 (P0067), and ATG7 (A2856) from Sigma-Aldrich; AGRP (AF634) from R&D Systems; p-PRKAA/p-AMPKα (Thr172, 2535), PRKAA/AMPKα (2532), p-ACAC (Ser79, 3661), ACAC (3662),

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