Klotho Antibody AF1819 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemKlotho Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1819
ApplicationsELISA Development,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetKlotho
Quantity100 ug (also 25 ug)
IsotypeIgG
HostGoat
Conjugate/TagUnconjugated
ReactivityCanine,Human,Mouse,Rat,Transgenic Mouse
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Klotho, Arg31-His550, Accession # BAA25307
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameklotho
NCBI Gene AliasesHFTC3, KLA

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Klotho in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

What species does this Klotho antibody work with in experiments?

The Klotho antibody has been shown to react with mouse, human, and rat samples, making it suitable for studies involving these species.

Can this antibody be used for immunohistochemistry, and if so, how has it been applied in research?

Yes, the Klotho antibody has been utilized in immunohistochemistry. For instance, in a study examining renal expression of Klotho, the antibody was used to visualize Klotho in kidney sections, demonstrating its application in assessing Klotho's role in kidney pathology ().

How effective is the Klotho antibody in detecting Klotho protein in Western blot assays?

The Klotho antibody has been effectively used in Western blot assays to detect Klotho protein levels, as evidenced by its application in various studies, including those investigating the role of Klotho in diabetic nephropathy and renal fibrosis (, ).

What are some specific applications of this antibody in recent research?

The Klotho antibody has been applied in various contexts, including studies on its protective effects against kidney injury (), its role in hypertension under Klotho deficiency (), and its involvement in modulating renal fibrosis through TGF-β signaling pathways ().

R&D Systems, a Bio-Techne Brand

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Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Pyrophosphate dysregulation and impaired FGF23/FGFR signaling contributes to impaired matrix mineralization in bone marrow stromal cultures from sickle cell disease miceBiochemical and Biophysical Research CommunicationsJanuary 18, 2026Liping Xiao, Marja M. Hurley,R&D SYSTEM,MAB26291,AB_2104623 pFGFR1,Abcam,ab59194,AB_941585 pFGFR2,Invitrogen,PA5-106140,AB_2817538 pFGFR3,Invitrogen,PA5-64798,AB_2662689 αKlotho,R&D SYSTEM,AF1819,AB_2296612 pSTAT3,Cell Signaling Technology,9145,AB_2491009 pAKT,Cell Signaling Technology,4060,AB_2315049 AKT,Cell Signaling Technology

(2) Sex-Related Expression of Klotho in Rat Kidneys: Species Differences Between Rats and MiceInternational journal of molecular sciencesJanuary 10, 2026Breljak D, Karaica D, Vrhovac Madunić I, Micek V, Orct T, Ljubojević M, Rašić D, Vogrinc Ž, Kralik S, Gerić M, et al.[36,37]" beta-actin,β-actin-Ab,"sc-47778 (Santa Cruz Biotechnology, Dallas, TX, USA) [32,35]" Kl,Kl-Ab,"AF1819 (R&D Systems, Minneapolis, MN, USA) [33]" Kl,Kl-Ab,"KO603; cloneKM2076 (TransGenicInc., Fukuoka, Japan)

(3) α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in MiceInternational Journal of Molecular SciencesDecember 30, 2025Lifang Zheng, Yinian Wang, Zirui Xiao, Zhijian Rao, Rengfei Shifollowing primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat #

(4) α-Klotho Promotes Cell Death and Suppresses Inflammation through TNF–Cellular Inhibitor of Apoptosis Protein 1 Signaling in Autosomal Dominant Polycystic Kidney DiseaseJ Am Soc NephrolDecember 3, 2025Linda Xiaoyan Li, Julie X. Zhou, Hongbing Zhang, James P. Calvet, Chou-Long Huang, Xiaogang LiKi67 antibody (ab15580) diluted at 1:200, which was purchased from Abcam; (4) anti-Klotho antibody (AF1819) diluted at 1:1000, which was purchased from R&D System; (5) anti-p65 (10745-1-AP), anti-GFP (50430-2-AP),

(5) Asparagine614 Determines the Transport and Function of the Murine Anti-Aging Protein KlothoCellsOctober 21, 2024Zahra Fanaei-Kahrani, Christoph Kaethercontaining 1 mM CaCl2 and 0.5 mM MgCl2). The coverslips were then incubated with anti-Klotho antibody (AF1819, R&D Systems, 1:50 dilution) for 30 min at 4 °C, rinsed 3 times with PBSI, and fixed with ROTI®Histofix

(6) Comparative Analysis of Acute Kidney Injury Models and Related Fibrogenic Responses: Convergence on Methylation Patterns Regulated by Cold Shock ProteinCellsFebruary 20, 2024Brandt S, Bernhardt A, Häberer S, Wolters K, Gehringer F, Reichardt C, Krause A, Geffers R, Kahlfuß S, Jeron A, et al.Tween–PBS) for 30 min at room temperature, and a primary antibody (goat anti-mouse KLOTHO, R&D Systems, AF1819; goat anti-mouse Fibrinogen, Santa Cruz, 18032) was added overnight at 4 °C diluted in 1% FCS plus 1×

(7) Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injuryFrontiers in PharmacologyJanuary 3, 2024Jie Xu, Enqing Lin, Xue Hong, Li Li, Jun Gu, Jinghong Zhao, Youhua Liusecondary antibodies for 1 h at room temperature. The primary antibodies are as follows: anti-mKlotho (AF1819; R&D Systems), anti-PARP (#9532; Cell Signaling Technology), anti-p53 (#2524S; Cell Signaling Technology),

(8) Deubiquitinating enzyme USP11 promotes renal tubular cell senescence and fibrosis via inhibiting the ubiquitin degradation of TGF-β receptor IIActa pharmacologica SinicaMarch 1, 2023Ni JY, Wang X, Xie HY, Yang NH, Li JY, Sun XA, Guo HJ, Zhou L, Zhang W, Liu J, et al.

(9) Soluble Klotho protects against glomerular injury through regulation of ER stress responseCommunications biologyFebruary 22, 2023Charrin E, Dabaghie D, Sen I, Unnersjö-Jess D, Möller-Hackbarth K, Burmakin M, Mencke R, Zambrano S, Patrakka J, Olauson H.donkey serum (C06SB, Bio-Rad) for 1 h at room temperature. Sections were incubated with anti-Klotho (AF1819; 1:200, R&D Systems) overnight at 4 °C and anti-synaptopodin (#61094; 1:700, PROGEN Biotechnik GmbH)

(10) Klotho Regulated by Estrogen Plays a Key Role in Sex Differences in Stress Resilience in RatsInternational journal of molecular sciencesJanuary 7, 2023Tan Z, Li Y, Guan Y, Iqbal J, Wang C, Yan R, Ma XM.Bristol, UK), letrozole (aromatase inhibitor, 4382, TOCRIS, Bristol, UK), and primary antibodies (Klotho (AF1819, R&D Systems, Minneapolis, MN, USA, immunostaining and Western blot), Klotho (ab203576, Abcam, Cambridge,

(11) KYA1797K, a Novel Small Molecule Destabilizing β-Catenin, Is Superior to ICG-001 in Protecting against Kidney AgingKidney diseases (Basel, Switzerland)November 1, 2022Zhu M, Ling X, Zhou S, Meng P, Chen Q, Chen S, Shen K, Xie C, Kong Y, Wang M, et al.BD Transduction Laboratories, USA), anti-PGC-1α (1:1,000; ab54481, Abcam, UK), anti-Klotho (1:1,000; AF1819, R&D Systems, USA), anti-α-smooth muscle actin (α-SMA) (1:3,000; a2547, Sigma-Aldrich), anti-α-SMA (1:1,000;

(12) Orally-active, clinically-translatable senolytics restore α-Klotho in mice and humansEBioMedicineMarch 1, 2022Zhu Y, Prata LGPL, Gerdes EOW, Netto JME, Pirtskhalava T, Giorgadze N, Tripathi U, Inman CL, Johnson KO, Xue A, et al.(Millipore Sigma). Membranes were immunoblotted with primary mouse α-Klotho antibody (R&D Systems, AF1819) at 1:1000 dilution and GAPDH antibody (Cell Signaling Technology, 97166) at 1:1000 dilution. Enhanced

(13) A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-β signalingNature communicationsJanuary 21, 2022Yuan Q, Ren Q, Li L, Tan H, Lu M, Tian Y, Huang L, Zhao B, Fu H, Hou FF, et al.#4695), anti-p38 (dilution 1:1000; #8690; Cell Signaling Technology), anti-Klotho (dilution 1:1000; AF1819; R&D System), anti-TβR2 (dilution 1:1000; sc-7791; Santa Cruz), anti-TGF-β1 (dilution 1:1000; ab92486;

(14) METTL14 promotes glomerular endothelial cell injury and diabetic nephropathy via m6A modification of α-klothoMolecular medicine (Cambridge, Mass.)September 9, 2021Li M, Deng L, Xu G.MDA (#EU2577, Wuhan Fine Biotech Co., Ltd.) levels in mice renal tissues and α-klotho (R&D Systems, #AF1819), TNF-α (R&D Systems, #MTA00B) and IL-6 (Sigma, #RAB0308-1KT) levels in mice serum were detected by

(15) Cannabinoid receptor 2 plays a central role in renal tubular mitochondrial dysfunction and kidney ageingJournal of cellular and molecular medicineSeptember 1, 2021Zhou S, Ling X, Meng P, Liang Y, Shen K, Wu Q, Zhang Y, Chen Q, Chen S, Liu Y, et al.(a2547; Sigma‐Aldrich), anti‐α‐SMA (ab5694; Abcam), anti‐β‐catenin (610154; BD Biosciences), anti‐Klotho (AF1819; R&D Systems), anti‐p14 ARF (ab185620; Abcam), anti‐p16INK4A (ab189034; Abcam), anti‐p19ARF (ab202225;

(16) Localization of fibroblast growth factor 23 protein in the rat hypothalamusThe European journal of neuroscienceAugust 1, 2021Ursem SR, Diepenbroek C, Bacic V, Unmehopa UA, Eggels L, Maya-Monteiro CM, Heijboer AC, la Fleur SE.(Vervloet, 2019). Sections were incubated with a polyclonal Klotho antibody: goat antimouse αKlotho 1/50 (AF1819, R&D Systems, Minneapolis, USA), after being washed in TRIS‐buffered saline (TBS; 50‐mM Tris‐Cl, 150‐mM

(17) The biphasic and age-dependent impact of klotho on hallmarks of aging and skeletal muscle functioneLifeApril 20, 2021Clemens Z, Sivakumar S, Pius A, Sahu A, Shinde S, Mamiya H, Luketich N, Cui J, Dixit P, Hoeck JD, et al.ab6586, RRID:AB_305584 IF(1:500) Antibody Anti-Klotho capture antibody (goat polyclonal) R and D Systems Cat#: AF1819, RRID:AB_2296612 MSD-ELISA(4 mg/mL) Antibody Anti-Klotho detection antibody (goat polyclonal) R and D Systems

(18) Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cellsPhysiological reportsJanuary 1, 2021Miao J, Huang J, Luo C, Ye H, Ling X, Wu Q, Shen W, Zhou L.visualized using an ECL kit (Applygen, Beijing, China). The primary antibodies were as follows: anti‐Klotho (AF1819; R&D Systems), anti‐α‐SMA (A2547; Sigma‐Aldrich), anti‐fibronectin (F3648; Sigma‐Aldrich), anti‐GAPDH

(19) Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiencyThe Journal of clinical investigationAugust 3, 2020Kawarazaki W, Mizuno R, Nishimoto M, Ayuzawa N, Hirohama D, Ueda K, Kawakami-Mori F, Oba S, Marumo T, Fujita T.minutes. The supernatant was collected and incubated at 4°C overnight with 1 μg anti-Klotho antibody (AF1819, R&D Systems). The next day, the sample was incubated at 4°C for 2 hours with 30 μL protein G-agarose

(20) Release of insulin produced by the choroid plexis is regulated by serotonergic signalingJCI insightDecember 5, 2019Mazucanti CH, Liu QR, Lang D, Huang N, O'Connell JF, Camandola S, Egan JM.(Cell Signaling Technology, 5309), SYT1 (Cell Signaling Technology, 14558), and klotho (KL) (R&D Systems, AF1819) at 4°C overnight. All primary antibodies were used at a 1:500 concentration. Antibody against β-actin

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Klotho Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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Klotho Antibody from R&D Systems, a Bio-Techne Brand

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