LIF Antibody AF449 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemLIF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF449
ApplicationsBioassay,ELISA Development,Western Blot,Neutralization,Immunocytochemistry
IsotypeIgG
Quantity100 ug (also 25 ug)
HostGoat
Conjugate/TagUnconjugated
TargetLIF
ReactivityMouse,Transgenic Mouse
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse LIF
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene NameLIF interleukin 6 family cytokine
NCBI Gene AliasesCDF, DIA, HILDA, MLPLI

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross-reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Enhancing uterine receptivity for embryo implantation through controlled collagenase interventionLife Science AllianceOctober 1, 2024Eldar Zehorai, Tamar Gross Lev, Elee Shimshoni, Ron Hadas, Idan Adir, Ofra Golani, Guillaume Molodij, Ram Eitan, Karl E Kadler, Orit Kollet, et al.analysis tool. Antibodies used in this study: VEGF-A (A-20) (sc-152; Santa Cruz Biotechnology), LIF (AF449; R&D systems), VEGF-R2 (55B11; cell signaling), NKp46 (AF2225; R&D systems), β-tubulin (sc-9104; Santa cruz

(2) Stress-induced β cell early senescence confers protection against type 1 diabetesCell MetabolismDecember 1, 2023Hugo Lee, Gulcan Semra Sahin, Chien-Wen Chen, Shreyash Sonthalia, Sandra Marín Cañas, Hulya Zeynep Oktay, Alexander T. Duckworth, Gabriel Brawerman, Peter J. Thompson, Maria Hatzoglou, et al.inhibitor; 666–15 (MedChem, #HY-101120), 4μ8C (Sigma, #SML0949), mouse LIF antibody (R&D systems, #AF449), and normal goat IgG control (R&D systems, #AB-108-C). In vivo administration of virus and antibodies 1×1012

(3) Neuroprotection against ischemic stroke requires a specific class of early responder T cells in miceThe Journal of clinical investigationAugust 1, 2022Cai W, Shi L, Zhao J, Xu F, Dufort C, Ye Q, Yang T, Dai X, Lyu J, Jin C, et al.then collected for further experiments. For LIFR blocking in the coculture, LIF-neutralizing antibody (AF449, R&D Systems) was added at 60 ng/mL. In some experiments, sorted CD8+ TRLs were treated with LIF (554004,

(4) Concomitant Activation of OSM and LIF Receptor by a Dual-Specific hlOSM Variant Confers Cardioprotection after Myocardial Infarction in MiceInternational journal of molecular sciencesDecember 29, 2021Lörchner H, Adrian-Segarra JM, Waechter C, Wagner R, Góes ME, Brachmann N, Sreenivasan K, Wietelmann A, Günther S, Doll N, et al.

(5) IL1-Induced JAK/STAT Signaling Is Antagonized by TGFβ to Shape CAF Heterogeneity in Pancreatic Ductal AdenocarcinomaCancer discoveryFebruary 1, 2019Biffi G, Oni TE, Spielman B, Hao Y, Elyada E, Park Y, Preall J, Tuveson DA.(11969S, Cell signaling) or an IgG control (sc-2027, Santa Cruz), 3.4 μg/mL LIF neutralizing antibody (AF449, R&D Systems) or an IgG control (AB-108-C, R&D), 3.8 μg/mL G-CSF (MAB414, R&D Systems) or IL-6 (MAB406,

(6) Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210Cell reportsJuly 31, 2018Tang Y, Xiong S, Yu P, Liu F, Cheng L.

(7) TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCsJournal of leukocyte biologyAugust 1, 2017Won H, Moreira D, Gao C, Duttagupta P, Zhao X, Manuel E, Diamond D, Yuan YC, Liu Z, Jones J, et al.

(8) Protein kinase inhibitor γ reciprocally regulates osteoblast and adipocyte differentiation by downregulating leukemia inhibitory factorStem CellsDecember 1, 2013Chen X, Hausman BS, Luo G, Zhou G, Murakami S, Rubin J, Greenfield EM.indicated concentrations of either a neutralizing affinity-purified goat anti-murine LIF antibody (AF449, R & D Systems, Minneapolis, MN) or a normal goat control immunoglobulin (AB-108-C, R & D Systems) was

(9) Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicityJournal of neuroinflammationAugust 16, 2012Moidunny S, Vinet J, Wesseling E, Bijzet J, Shieh CH, van Ijzendoorn SC, Bezzi P, Boddeke HW, Biber K.Netherlands) were coated overnight at room temperature with 100 μl/well of primary antibody goat anti-LIF (AF449; 0.5 μg/mL, R&D Systems, Oxford, UK) diluted in 0.01 M PBS (pH 7.4). The following day, the plates were

(10) Leukemia inhibitory factor signaling is required for lung protection during pneumoniaJournal of immunology (Baltimore, Md. : 1950)June 15, 2012Quinton LJ, Mizgerd JP, Hilliard KL, Jones MR, Kwon CY, Allen E.

(11) Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune diseaseImmunityAugust 26, 2011Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, et al.

(12) Astrocytes promote myelination in response to electrical impulsesNeuronMarch 16, 2006Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD.

(13) The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in miceArch Dermatol ResNovember 1, 2001Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M.

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LIF Antibody

LIF Antibody

LIF Antibody

LIF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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LIF Antibody from R&D Systems, a Bio-Techne Brand

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