BMP-2/BMP-4 Antibody AF355 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemBMP-2/BMP-4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF355
NCBI Gene AliasesSSFSC1, BDA2, SSFSC, BMP2A
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry
Conjugate/TagUnconjugated
ReactivityCanine,Hamster,Human,Mouse
TargetBMP-2/BMP-4
IsotypeIgG
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
NCBI Full Gene Namebone morphogenetic protein 2
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant human BMP-2, Gln283-Arg396, Accession # P12643
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects BMP-2 and BMP-4 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study BMP-2 in the context of bone remodeling disorders like otosclerosis?

Yes, the BMP-2/BMP-4 antibody has been shown to work in immunofluorescent assays to detect BMP-2 expression in histological samples from otosclerotic foci, correlating with the histological activity of the condition as reported in the study by Csomor et al. (2012).

Is the BMP-2/BMP-4 antibody effective for use in canine mammary tumor research?

The antibody has been utilized in studies investigating BMP expression profiles in various canine mammary tumors, where it was found to be effective in detecting BMP-2 and BMP-4, particularly in tumors expressing high levels of these proteins, as noted in the research by Wensman et al. (2009).

How does the BMP-2/BMP-4 antibody perform in immunohistochemistry for human tissues?

The antibody has been successfully employed in immunohistochemical staining of human tissues, including breast cancer samples, where it was associated with the expression of BMP-2 and its correlation with clinicopathological features, as demonstrated in the study by Zhang et al. (2019).

What species can this antibody be used on for research purposes?

The BMP-2/BMP-4 antibody is reactive with multiple species, including humans, mice, hamsters, and dogs, making it versatile for various experimental models in cell biology and related research.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Shape-fit 3D printed mineralized membrane for guided bone regeneration in long bone defectsJ NanobiotechnologyDecember 22, 2025Wenbin Jiang, Yichen Zhan, Yuxuan Chen, Jiaming Sun, Qiuning Lin, Qiang He, Junpeng Ma, Bingkun Bao, Zhenxing Wangagainst CD86 (ab955, Abcam, 1:200), CD163 (ab182422, Abcam, 1:200), RUNX2 (ab76956, Abcam, 1:200), BMP2 (AF355, R&D Systems, 1:100), and OCN (ab13418, Abcam, 1:200). Tissue sections were incubated with primary antibodies

(2) Distribution of Immunomodulation, Protection and Regeneration Factors in Cleft-Affected Bone and CartilageDiagnostics (Basel)October 4, 2024Mārtiņš Vaivads, Māra Pilmanerabbit, polyclonal, dilution 1:100, Novus Biologicals, LLC, Centennial, CO, USA); and BMP-2/4 antibodies (AF355, goat, polyclonal, dilution 1:100, R&D Systems, Inc., Minneapolis, MN, USA). The nonparametric evaluation

(3) Amlodipine accelerates bone healing in a stable closed femoral fracture model in miceEuropean cells & materialsMay 24, 2021Menger MM, Merscher B, Scheuer C, Braun BJ, Herath SC, Rollmann MF, Stenger D, Später T, Pohlemann T, Menger MD, et al.detection was performed using the following antibodies: goat anti-mouse BMP-2/BMP-4 (1 : 100, R&D Systems, AF355), sheep anti-mouse CYR61 (1 : 100, R&D Systems, AF4055), rabbit anti-mouse VEGF (1 : 300, Abcam, ab3109),

(4) Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells: BMP-2 and BMP-7Scientific reportsFebruary 9, 2021Kim MG, Kim CL, Kim YS, Jang JW, Lee GM.stained with the primary antibodies, goat anti‐human BMP‐2 antibody (R&D systems, Minneapolis, MN; AF355) and mouse anti-HS antibody (Amsbio, Abingdon, UK; 370255) overnight at 4 °C, followed by secondary

(5) Microcalcification and BMP-2 in breast cancer: correlation with clinicopathological features and outcomesOncoTargets and therapyJanuary 1, 2019Zhang L, Hao C, Wu Y, Zhu Y, Ren Y, Tong Z.with hydrogen peroxide and normal goat serum, the sections were incubated with monoclonal anti-BMP-2 (AF355, R&D Systems, Inc., Minneapolis, MN, USA; 1:500), monoclonal anti-OPN (sc-21742, Santa Cruz Biotechnology Inc

(6) Evaluation of the presence of MMP-2, TIMP-2, BMP2/4, and TGFβ3 in the facial tissue of children with cleft lip and palateActa medica LituanicaJanuary 1, 2018Smane L, Pilmane M.anti-TGFβ3 at 1:200 (sc82; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), goat anti-BMB2/4 at 1:100 (AF355; R&D Systems, Inc., Minneapolis, USA). For the immunohistochemical staining protocol, the following

(7) Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic fociActa oto-laryngologicaJune 1, 2012Csomor P, Liktor B, Liktor B, Szekanecz Z, Sziklai I, Karosi T.1:20 polyclonal goat anti-human BMP2/4 primary antibody solution (20 C, 24 h, continuous shaking) (RD-AF355, R&D Systems), with 1:20 polyclonal goat antihuman BMP5 primary antibody solution (20 C, 24 h, continuous

(8) Midbrain dopamine neurons associated with reward processing innervate the neurogenic subventricular zoneThe Journal of neuroscience : the official journal of the Society for NeuroscienceSeptember 14, 2011Lennington JB, Pope S, Goodheart AE, Drozdowicz L, Daniels SB, Salamone JD, Conover JC.Bioscience Research Reagents; Triton X-100 exposure in blocking phase only); anti-Caspase3 (6 ␮g/ml, cat. #AF355, R&D Systems), anti-GABA (44 ␮g/ ml, #55545, Sigma), anti-tyrosine hydroxylase (1:500, cat. #ab113,

(9) Diverse bone morphogenetic protein expression profiles and smad pathway activation in different phenotypes of experimental canine mammary tumorsPloS oneSeptember 22, 2009Wensman H, Heldin NE, Pejler G, Hellmén E.Smad-1/5 (PS1)[19], a BMP-6 antibody (ab15640, Abcam, Cambridge, UK) and a polyclonal BMP-2/4 antibody (AF355, R&D systems, Europe Ltd, Abingdon, UK). The slides were incubated in 1% H2O2 in methanol for 30 min

(10) The effect of host tissue irradiation on large-segment allograft incorporationClinical orthopaedics and related researchJune 1, 2005Ehrhart NP, Eurell JA, Constable PD, Gaddy D, Nicholas RW.

(11) Disruption of LTBP-4 function reduces TGF-beta activation and enhances BMP-4 signaling in the lungThe Journal of cell biologyOctober 11, 2004Koli K, Wempe F, Sterner-Kock A, Kantola A, Komor M, Hofmann WK, von Melchner H, Keski-Oja J.Cruz Biotechnology, Inc. BMP-4 antibody used in immunohistochemistry was obtained from R&D Systems (AF355). Anti-fibronectin antibodies used for immunofluorescence and immunohistochemistry were purchased from

(12) HOXA13 regulates the expression of bone morphogenetic proteins 2 and 7 to control distal limb morphogenesisDevelopment (Cambridge, England)September 1, 2004Knosp WM, Scott V, Bächinger HP, Stadler HS.

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BMP-2/BMP-4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (12)

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BMP-2/BMP-4 Antibody from R&D Systems, a Bio-Techne Brand

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