Human VEGFR1/Flt-1 Antibody AF321 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman VEGFR1/Flt-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF321
NCBI Gene AliasesFLT-1, VEGFR1, FLT, VEGFR-1
ApplicationsBlocking,Proximity Ligation Assay (PLA),Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready,Functional Assay
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
ReactivityBovine,Chinese Hamster,Human
TargetVEGFR1/Flt-1
IsotypeIgG
NCBI Full Gene Namefms related receptor tyrosine kinase 1
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1, Ser27-His687, Accession # NP_001153392
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Myeloid-T cell proximity is prominent in healthy pregnancies with extreme fetal-maternal HLA incompatibilityiScienceJanuary 16, 2026Xuezi Tian, Juliette Krop, Marieke E. Ijsselsteijn, Johanna M. Kapsenberg, Jacqueline D.H. Anholts, Lotte van der Meeren, Hailiang Mei, Michiel H.J. Huigen, Carin van der Keur, Dave L. Roelen, et al.Technology,D6E9W C1q,Abcam,C1QA/2956 Ki-67,Cell Signaling Technology,8D5 CD3,Abcam,EP449E CD66b,BioLegend,G10F5 Flt-1,R&D Systems,AF321 HLA-G,Exbio,MEM-G2 CD39,Abcam,EPR20627 CD1c,Abcam,EPR23189-196 CD16,Cell Signaling Technology,D1N9L CD138,BioLegend,MI15 DC-SIGN,Novus Biologicals

(2) In vivo inhibition of angiogenesis by htsFLT01/MiRGD nano complexTranslational oncologyJune 1, 2025Khoshandam M, Soheili ZS, Hosseinkhani S, Samiee S, Latifi-Navid H, Ahmadieh H, Soltaninejad H, Jahangiri B.temperature. Following washes with TBST, the membrane was exposed to the primary antibody (R&D systems, cat# AF321-S) overnight in the refrigerator, followed by three washes with TBST. The membrane was then incubated

(3) Endomucin selectively regulates vascular endothelial growth factor receptor-2 endocytosis through its interaction with AP2Cell communication and signaling : CCSApril 11, 2024Cano I, Wild M, Gupta U, Chaudhary S, Ng YSE, Saint-Geniez M, D'Amore PA, Hu Z.#4696S), rabbit anti-VEGFR2 (1:1,000, Cell Signaling, #2479S), goat anti-VEGFR1 (1:1000, R&D Systems #AF321), rabbit anti-FGFR1 (1:1000, Cell Signaling, #9740S), mouse anti-Myc (1:1000, Cell Signaling, #9B11)

(4) MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and PreeclampsiaInternational journal of molecular sciencesJune 13, 2023Yong Q, Dijkstra KL, van der Keur C, Bruijn JA, Eikmans M, Baelde HJ.Immunochemistry was performed using the following primary antibodies: goat anti-human FLT-1 (1:100, AF321, R&D Systems, Minneapolis, MN, USA), rabbit anti-human MIF (1:200, ABP51791, Abbkine, Wuhan, China),

(5) sFlt-1-enriched exosomes induced endothelial cell dysfunction and a preeclampsia-like phenotype in miceCytokineJune 1, 2023Huang X, Jia L, Jia Y, Xu X, Wang R, Wei M, Li H, Peng H, Wei Y, He Q, et al.

(6) Design, construction and in vivo functional assessment of a hinge truncated sFLT01Gene therapyApril 1, 2023Zakeri F, Latifi-Navid H, Soheili ZS, Sadeghi M, Arab SS, Samiei S, Pirmardan ER, Taghizadeh S, Ahmadieh H, Hafezi-Moghadam A.

(7) Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophagesBMC cancerMarch 20, 2023Liu X, Li Z, Sun J, Zhang Z, Li W.analysis. In some experiments, anti-VEGFR1 neutralizing antibody (NAb) (25 µg/mL, Novus Biologicals, Cat: AF321), anti-IFN-γ NAb (10 µg/mL, eBioscience, Cat: 16-7318-81), sVEGFR1 (100 ng/mL, Biolegend, Cat: 555,802)

(8) Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementiaJournal of cellular and molecular medicineJune 1, 2022Mitroi DN, Tian M, Kawaguchi R, Lowry WE, Carmichael ST.AF1097, 5 µg/ml, goat antibody), vascular endothelial growth factor receptor 1 (FLT1) (R&D System, cat# AF321, 20 µg/ml, goat antibody), tight junction protein claudin‐5 (CLND5) (Abcam, cat# ab15106, 1:30, rabbit

(9) Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and TelocytesInternational journal of molecular sciencesMay 31, 2022Wrona A, Aleksandrovych V, Bereza T, Basta P, Gil A, Ulatowska-Białas M, Mazur-Laskowska M, Pityński K, Gil K.

(10) Salvianolic acid B and ferulic acid synergistically promote angiogenesis in HUVECs and zebrafish via regulating VEGF signalingJournal of ethnopharmacologyJanuary 30, 2022Chen J, Wang Y, Wang S, Zhao X, Zhao L, Wang Y.

(11) Oviductal Telocytes in Patients with Uterine MyomaBiomedicinesAugust 20, 2021Aleksandrovych V, Wrona A, Bereza T, Pityński K, Gil K.receptor,"NCL-L-ER-6F11, Leica Biosystems, Newcastle upon Tyne, UK",1:50 Polyclonal goat anti-VEGF R1/Flt-1,"AF321, R&D Systems, Minneapolis, Minnesota, USA",5 μg/mL Monoclonal mouse anti-HIF-1,"ab16066, Abcam, Cambridge,

(12) Molecular investigation of brain tumors progressing during pregnancy or postpartum period: the association between tumor type, their receptors, and the timing of presentationClinical neurology and neurosurgeryAugust 1, 2021Ichimura S, Ohara K, Kono M, Mizutani K, Kitamura Y, Saga I, Kanai R, Akiyama T, Toda M, Kohno M, et al.EGFR, anti-human HER-2 antibody (CB11, 1:100, Novocastra) for HER-2, anti-human VEGFR-1/Flt-1 antibody (AF321, 1:100, R&D Systems, Minneapolis, MN, USA) for VEGFR-1, anti-human VEGFR-2 antibody (55B11, 1:200, Cell

(13) Low-Dose Recombinant Adeno-Associated Virus-Mediated Inhibition of Vascular Endothelial Growth Factor Can Treat Neovascular Pathologies Without Inducing Retinal VasculitisHuman gene therapyJuly 1, 2021Cheng SY, Luo Y, Malachi A, Ko J, Su Q, Xie J, Tian B, Lin H, Ke X, Zheng Q, et al.anti-VCAM1 (1:400; Cell Signaling Technology; Cat no. 39036), goat anti-hVEGFR1 (1:300; R&D Systems; Cat no. AF321), rat anti-MHC Class II (1:1,000; BD Pharmingen; Cat no. 556999), rat anti-CD4 (1:300; BD Pharmingen;

(14) Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF‑1α and VEGF expressionMolecular medicine reportsJanuary 1, 2021Dong D, Fu Y, Chen F, Zhang J, Jia H, Li J, Wang H, Wen J.neutralization test, 25 µg/ml VEGF, 30 µg/ml VEGFR1 or 500 ng/ml VEGFR2 neutralization antibodies (cat. nos. AF293, AF321 and MAB3572; R&D Systems China Co., Ltd.) were added to cell culture media (37˚C, 5% CO2) at 2 h prior

(15) FKBPL and SIRT-1 Are Downregulated by Diabetes in Pregnancy Impacting on Angiogenesis and Endothelial FunctionFrontiers in endocrinologyJanuary 1, 2021Alqudah A, Eastwood KA, Jerotic D, Todd N, Hoch D, McNally R, Obradovic D, Dugalic S, Hunter AJ, Holmes VA, et al.SIRT-1 (Cat. no.: ab110304; Abcam, UK), PlGF (Cat. no.: ab180734, Abcam, UK) and VEGF-R1 (Cat. no.: AF321, R&D systems, USA). Tissue slides were imaged using a Leica DMi8 ﬂuorescence inverted microscope using

(16) Quantification of Trastuzumab-HER2 Engagement In Vitro and In VivoMolecules (Basel, Switzerland)December 17, 2020Rudkouskaya A, Smith JT, Intes X, Barroso M.

(17) Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signalingCell death & diseaseMay 4, 2020Wei ST, Huang YC, Hsieh ML, Lin YJ, Shyu WC, Chen HC, Hsieh CH.PDGFRα (MAB322, R&D Systems, 1:2000 dilution), PDGFRβ (MAB1263, R&D Systems, 1:1000 dilution), VEGFR1 (AF321, R&D Systems, 1:1000 dilution), VEGFR2 (AF357, R&D Systems, 1:1000 dilution), VEGFR3 (AF349, R&D Systems,

(18) BMP9 signaling promotes the normalization of tumor blood vesselsOncogeneApril 1, 2020Viallard C, Audiger C, Popovic N, Akla N, Lanthier K, Legault-Navarrete I, Melichar H, Costantino S, Lesage S, Larrivée B.

(19) Alterations of the tumor microenvironment in glioblastoma following radiation and temozolomide with or without bevacizumabAnnals of translational medicineMarch 1, 2020Tamura R, Tanaka T, Morimoto Y, Kuranari Y, Yamamoto Y, Takei J, Murayama Y, Yoshida K, Sasaki H.antibody were used: anti-VEGF-A (1:200, JH121, Merck Millipore), anti-VEGF receptor (VEGFR)1 (1:200, AF321, R&D SYSTEMS), anti-VEGFR2 (1:600, 55B11, Cell Signaling Technology), anti-CD34 (1:100, F1604, Nichirei

(20) Soluble fms-Like Tyrosine Kinase 1 Localization in Renal Biopsies of CKDKidney international reportsDecember 1, 2019Zsengellér ZK, Lo A, Tavasoli M, Pernicone E, Karumanchi SA, Rosen S.a 1:200 dilution of primary sFLT1 that recognizes the N-terminal region of FLT1/sFLT1 (catalog no. AF321; R&D Systems, Framingham, MA), and CD34 or CD68 antibodies (Agilent, Santa Clara, CA). Specific labeling

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VEGFR1/Flt-1 Antibody

VEGFR1/Flt-1 Antibody

VEGFR1/Flt-1 Antibody

VEGFR1/Flt-1 Antibody

Human VEGFR1/Flt-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (77)

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Human VEGFR1/Flt-1 Antibody from R&D Systems, a Bio-Techne Brand

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