DEP-1/CD148 Antibody AF1934 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemDEP-1/CD148 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF1934
ApplicationsIn Situ PLA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunocytochemistry
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Primate - Cercopithecus aethiops (African Green Monkey),Rat,Transgenic Mouse
TargetDEP-1/CD148
Conjugate/TagUnconjugated
NCBI Full Gene Nameprotein tyrosine phosphatase receptor type J
ClonalityPolyclonal
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ImmunogenE. coli-derived recombinant human DEP-1/CD148, Arg997-Ala1337, Accession # Q12913
NCBI Gene AliasesR-PTP-J, R-PTP-ETA, SCC1, DEP1, HPTPeta, HPTP eta, CD148

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human, mouse, and rat DEP-1/CD148 in Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) MiR-362-3p inhibits the proliferation, migration and EMT of gastric cancer cells by regulating the DEP-1/ERK signaling pathwayScientific ReportsMarch 30, 2026Fei Tu, Zhiyuan Li, Lina Yao, Fengyuan He, Yiqing Jia, Lingzhu Wang, Tiesuo Zhao, Sheng Guo, Yan Jin, Zhijun Yang(protein-tech, 60008-1-Ig), α-tubulin (CST, #3873), GAPDH (protein-tech, 60004-1-Ig), DEP-1 (R&D Systems, AF1934), E-cadherin (CST,#3195), Vimentin (Santa Cruz, sc-6260), p-p44/42 (CST, #4370), p44/42 (CST, #4695). Cell

(2) A Circulating GPNMB-Based Multimodal Model Integrates Tumor−Immune Crosstalk to Predict Immunotherapy Response in Esophageal CancerCancer DiscovFebruary 27, 2026Liang Zhu, Xiaoyuan Wang, Guoyu Cheng, Yancheng Lai, Lan Lan, Zhenghao Dong, Zhixuan You, Xinjie Chen, Ziyi He, Xinyi Xiao, et al.(Thermo Fisher Scientific, 88804). Briefly, lysates were incubated with anti-CD148 antibody (R&D Systems, AF1934, RRID: AB_355063) or isotype IgG, followed by binding to Protein A/G Magnetic Beads. To ensure compatibility

(3) Protocol to isolate glycoproteins from cultured mammalian cells and tissue samples using wheat germ agglutinin precipitationSTAR ProtocJuly 17, 2025Simran Chopra, Otsuware Linda-Josephine Kadiri, Kai Kappert, André Kleinriddersbuffer)",Santa Cruz,Cat#sc-47778 HRP; RRID: AB_2714189 DEP-1 (1 μg/mL in 2.5% BSA),R&D Systems,Cat#AF1934; RRID: AB_355063 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(4) DEP-1 is a brain insulin receptor phosphatase that prevents the simultaneous activation of counteracting metabolic pathwaysCell reportsDecember 24, 2024Chopra S, Kadiri OL, Ulke J, Hauffe R, Jonas W, Cheshmeh S, Schmidt L, Bishop CA, Yagoub S, Schell M, et al.

(5) The effects of CD148 Q276P/R326Q polymorphisms in A431D epidermoid cancer cell proliferation and epidermal growth factor receptor signalingCancer reports (Hoboken, N.J.)September 1, 2022He L, Takahashi K, Pasic L, Narui C, Ellinger P, Grundmann M, Takahashi T.interact with its ligand TSP1. 2 METHODS 2.1 Antibodies Immunoblotting: Anti‐CD148 goat polyclonal (AF1934) was from R&D Systems (Minneapolis, MN). Anti‐HA rat monoclonal was from Roche (Indianapolis, IN). Anti‐β

(6) Disrupting PTPRJ transmembrane-mediated oligomerization counteracts oncogenic receptor tyrosine kinase FLT3 ITDFrontiers in oncologyJanuary 1, 2022Schwarz M, Rizzo S, Paz WE, Kresinsky A, Thévenin D, Müller JP.AF768) recognizing the extracellular domain of the murine protein, anti-DEP-1 antibody (from goat, AF1934) recognizing murine DEP-1 and cross-reacting with human DEP-1 were obtained from R&D Systems (Wiesbaden,

(7) PTPRJ Inhibits Leptin Signaling, and Induction of PTPRJ in the Hypothalamus Is a Cause of the Development of Leptin ResistanceScientific reportsSeptember 14, 2017Shintani T, Higashi S, Suzuki R, Takeuchi Y, Ikaga R, Yamazaki T, Kobayashi K, Noda M.Anti-phosphotyrosine (4G10) and anti-NeuN (A60) antibodies were from Merck Millipore. The anti-PTPRJ (AF1934) antibody was from R&D Systems. Anti-SH2B1 (E-20) and anti-OB-R (B-3) antibodies were from Santa Cruz Biotechnology

(8) Enhanced insulin signaling in density-enhanced phosphatase-1 (DEP-1) knockout miceMolecular metabolismApril 1, 2015Krüger J, Brachs S, Trappiel M, Kintscher U, Meyborg H, Wellnhofer E, Thöne-Reineke C, Stawowy P, Östman A, Birkenfeld AL, et al.DEP-1 by using a radioactive labeled peptide was measured after immunoprecipitation with anti-DEP-1 (AF1934, 1 μg per condition, R&D Systems, Wiesbaden, Germany) in different metabolic tissues as described elsewhere

(9) Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factorCellular signallingJune 1, 2014Spring K, Lapointe L, Caron C, Langlois S, Royal I.

(10) Targeting density-enhanced phosphatase-1 (DEP-1) with antisense oligonucleotides improves the metabolic phenotype in high-fat diet-fed miceCell communication and signaling : CCSJuly 26, 2013Krüger J, Trappiel M, Dagnell M, Stawowy P, Meyborg H, Böhm C, Bhanot S, Ostman A, Kintscher U, Kappert K.to a final concentration of 50 mM. For immunoprecipitation PTP analyses anti-DEP-1 and anti-PTP1B (AF1934 and AF3954 R&D Systems, Wiesbaden, Germany) were used at a concentration of 1 μg in an end-over-end

(11) Association of the protein-tyrosine phosphatase DEP-1 with its substrate FLT3 visualized by in situ proximity ligation assayPloS oneJanuary 1, 2013Böhmer SA, Weibrecht I, Söderberg O, Böhmer FD.antibodies S18 (SC-480) from Santa Cruz Biotechnology (Heidelberg, Germany), polyclonal goat anti-DEP-1 (AF1934) antibodies from R&D Systems (Wiesbaden, Germany), rabbit monoclonal anti-pERK1/2 (#4695) and rabbit

(12) Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signalingThe Journal of biological chemistryApril 1, 2011Arora D, Stopp S, Böhmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Rönnstrand L, Tänzer S, Bauer R, et al.

(13) "T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases""Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Clayton KL, Haaland MS, Douglas-Vail MB, Mujib S, Chew GM, Ndhlovu LC, Ostrowski MA."Cat#AF2365), anti-Lck (Abcam; Cat#ab3885), anti-CD45 (Abcam; Cat#ab33533), anti-CD148 (R&D Systems; Cat#AF1934) and anti-CD3 (Abcam; Cat#ab5690) antibodies followed by the appropriate light-chain-specific secondary

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DEP-1/CD148 Antibody

DEP-1/CD148 Antibody

DEP-1/CD148 Antibody

DEP-1/CD148 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (13)

Reviews

DEP-1/CD148 Antibody from R&D Systems, a Bio-Techne Brand

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