Human/Mouse/Rat/Canine ALCAM/CD166 Antibody AF1172 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman/Mouse/Rat/Canine ALCAM/CD166 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1172
Quantity100 ug (also 25 ug)
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunofluorescence,Immunocytochemistry,CyTOF-ready
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ConcentrationLYOPH
PurityAntigen Affinity-purified
TargetALCAM/CD166
HostGoat
ReactivityCanine,Human,Mouse,Rat,Transgenic Mouse
IsotypeIgG
Antibody TypePrimary Antibodies
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166, Trp28-Lys527, Accession # AAC06342
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Nameactivated leukocyte cell adhesion molecule
NCBI Gene AliasesCD166, MEMD

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Myelin–axon interface vulnerability in Alzheimer’s disease revealed by subcellular proteomics and imaging of human and mouse brainNature NeuroscienceJuly 1, 2025Yifei Cai, Iguaracy Pinheiro-de-Sousa, Mykhaylo Slobodyanyuk, Fuyi Chen, Tram Huynh, Jean Kanyo, Peiyang Tang, Lukas A. Fuentes, Amber Braker, Rachel Welch, et al.RRID: AB_2035023), NRXN3 (1:200, Atlas Antibodies, HPA002727, RRID: AB_1079468), ALCAM (1:200, Novus AF1172, RRID: AB_354644), LSAMP (1:200, Atlas Antibodies, HPA076122, RRID: AB_2686779), HAPLN1 (1:200, Atlas Antibodies

(2) Comparison of functional characterization of cancer stem cells in different tumor tissues of pseudomyxoma peritoneiJournal of translational medicineNovember 14, 2024Zhou H, Xu H, Pang S, An L, Shi G, Wang C, Zhang P, Fan X, Yang J, Tang S, et al.#SAB5701045, Sigma-Aldrich, St. Louis, Missouri, USA) and polyclonal mouse anti-CD166 antibody (1:50, Cat. #AF1172, R&D Systems, Minneapolis, Minnesota, USA), were employed. Cell nuclei were counterstained with 4′6-diamidino-2-phenylindole

(3) VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammationNature communicationsSeptember 20, 2023Mapunda JA, Pareja J, Vladymyrov M, Bouillet E, Hélie P, Pleskač P, Barcos S, Andrae J, Vestweber D, McDonald DM, et al.anti-mouse PECAM-1 (in house, clone MEC13.3), goat-anti-human/mouse/rat/canine ALCAM (R&D systems, Cat No AF1172), rabbit-anti-mouse VE-cadherin (provided by Prof. Dietmar Vestweber, Max Planck Institute, Münster,

(4) Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during agingNat AgingJuly 1, 2023Yeo RW, Zhou OY, Zhong BL, Sun ED, Navarro Negredo P, Nair S, Sharmin M, Ruetz TJ, Wilson M, Kundaje A, et al.

(5) CD166-specific CAR-T cells potently target colorectal cancer cellsTranslational oncologyJanuary 1, 2023He S, Li S, Guo J, Zeng X, Liang D, Zhu Y, Li Y, Yang D, Zhao X.sodium azide (at a density of 1 × 106 cells/ml). Subsequently, CD166 primary antibody (R&D system, AF1172) was added to the cell suspension according to the manufacturer's instructions and incubated for 2 h

(6) Neural cell adhesion molecule is required for ventricular conduction system developmentDevelopment (Cambridge, England)June 1, 2021Delgado C, Bu L, Zhang J, Liu FY, Sall J, Liang FX, Furley AJ, Fishman GI.NCAM-1 (1:100, rabbit, Millipore, ab5032; rat, Millipore, mab310); ALCAM (1:50, goat, R&D Systems, AF1172); CNTN2 (1:50, goat, R&D Systems, AF4439); Cx40 (1:500, rabbit, Alpha Diagnostic, Cx40A); PSA (1:500,

(7) A comprehensive library of human transcription factors for cell fate engineeringNature biotechnologyApril 1, 2021Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, Shipman SL, Appleton E, Kiaee K, Kohman RE, et al.

(8) Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166Nature communicationsMarch 19, 2020Renard HF, Tyckaert F, Lo Giudice C, Hirsch T, Valades-Cruz CA, Lemaigre C, Shafaq-Zadah M, Wunder C, Wattiez R, Johannes L, et al.immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology

(9) Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells Can Be Enriched by a Combination of Mitochondrial Content and Activated Leukocyte Cell Adhesion MoleculeJMA journalSeptember 4, 2019Yamashita H, Fukuda K, Hattori F.

(10) Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and SemaphorinsThe Journal of neuroscience : the official journal of the Society for NeuroscienceAugust 21, 2019Bye CR, Rytova V, Alsanie WF, Parish CL, Thompson LH.

(11) ALCAM Mediates DC Migration Through Afferent Lymphatics and Promotes Allospecific Immune ReactionsFrontiers in immunologyJanuary 1, 2019Willrodt AH, Salabarria AC, Schineis P, Ignatova D, Hunter MC, Vranova M, Golding-Ochsenbein AM, Sigmund E, Romagna A, Strassberger V, et al.had emigrated from untreated biopsies by co-staining with polyclonal goat anti-ALCAM (R&D Systems, AF1172) or corresponding isotype control (R&D Systems), followed by AlexaFluor594-conjugated secondary antibodies

(12) Distribution and development of molecularly distinct perineuronal nets in visual thalamusJournal of neurochemistryDecember 1, 2018Sabbagh U, Monavarfeshani A, Su K, Zabet-Moghadam M, Cole J, Carnival E, Su J, Mirzaei M, Gupta V, Salekdeh GH, et al.RRID:AB_90715] 1:5000, CALB1 [Swant, CB‐38a] 1:10000, CALB2 [Chemicon AB5054] 1:5000, ALCAM [R&D System AF1172] 1:1000, SEZ6l [R&D System AF4804] 1:1000, ACAN [Millipore AB1031] 1:500, ACTIN [EMD Millipore MAB1501,

(13) Tbr1 instructs laminar patterning of retinal ganglion cell dendritesNature neuroscienceMay 1, 2018Liu J, Reggiani JDS, Laboulaye MA, Pandey S, Chen B, Rubenstein JLR, Krishnaswamy A, Sanes JR.

(14) Multicolor quantitative confocal imaging cytometryNature methodsJanuary 1, 2018Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T.

(15) Cell Adhesion Molecule CD166/ALCAM Functions Within the Crypt to Orchestrate Murine Intestinal Stem Cell HomeostasisCellular and molecular gastroenterology and hepatologyMay 1, 2017Smith NR, Davies PS, Levin TG, Gallagher AC, Keene DR, Sengupta SK, Wieghard N, El Rassi E, Wong MH.for IHC/immunofluorescence,Antibodies for IHC/immunofluorescence,Antibodies for IHC/immunofluorescence CD166,R&D Systems,AF1172,500,N/A GFP,Life Technologies,A11122,500,N/A GFP,Aves,GFP-1020,1000,N/A Lysozyme,DAKO,A0099,500,N/A Lysozyme,The

(16) Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasisProceedings of the National Academy of Sciences of the United States of AmericaJanuary 24, 2017Lécuyer MA, Saint-Laurent O, Bourbonnière L, Larouche S, Larochelle C, Michel L, Charabati M, Abadier M, Zandee S, Haghayegh Jahromi N, et al.G (ThermoFisher Scientific) were coupled to 10 μg of polyclonal goat anti-mouse ALCAM (R&D Systems, AF1172) or to 10 μg of irrelevant goat IgG (R&D Systems) following the E532 | www.pnas.org/cgi/doi/10.1073/pnas.1614336114 manufacturer’s

(17) Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitorsProceedings of the National Academy of Sciences of the United States of AmericaApril 14, 2015Bye CR, Jönsson ME, Björklund A, Parish CL, Thompson LH.

(18) Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglionPloS oneJanuary 1, 2015Gu Z, Imai F, Kim IJ, Fujita H, Katayama Ki, Mori K, Yoshihara Y, Yoshida Y.

(19) Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in miceNature communicationsMarch 18, 2014Leibowitz BJ, Wei L, Zhang L, Ping X, Epperly M, Greenberger J, Cheng T, Yu J.were incubated overnight at 4 °C in a humidified chamber with goat-anti-mouse CD166 antibodies (1:100; AF1172; R&D Systems, Minneapolis, MN). Sections were then incubated with AlexaFluor-594 rabbit-anti-goat secondary

(20) Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axonsDevelopment (Cambridge, England)March 1, 2014Inamata Y, Shirasaki R.

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ALCAM/CD166 Antibody

ALCAM/CD166 Antibody

ALCAM/CD166 Antibody

ALCAM/CD166 Antibody

ALCAM/CD166 Antibody

Human/Mouse/Rat/Canine ALCAM/CD166 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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Human/Mouse/Rat/Canine ALCAM/CD166 Antibody from R&D Systems, a Bio-Techne Brand

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