CXCL2/GRO beta/MIP-2/CINC-3 Antibody AF-452-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCXCL2/GRO beta/MIP-2/CINC-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-452-NA
ApplicationsBioassay,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunocytochemistry,Functional Assay
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
ReactivityMouse,Transgenic Mouse
HostGoat
TargetCXCL2/GRO beta/MIP-2/CINC-3
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse CXCL2/MIP-2, Ala28-Asn100, Accession # P10889
ConcentrationLYOPH
PurityAntigen Affinity-purified
NCBI Full Gene Namechemokine (C-X-C motif) ligand 2, C-X-C motif chemokine ligand 2
NCBI Gene AliasesSCYB2, Mgsa-b, CINC-2a, GROb, MGSA-b, Scyb2, MIP2A, MIP2, Scyb, Mip2, MIP-2a, GRO2, Gro2, MIP-2
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) GROß, recombinant mouse (rm) GRO gamma, rmKC and rmLIX is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of CXCL2 in inflammation during Mycobacterium tuberculosis infection?

Yes, the CXCL2/GRO beta/MIP-2 antibody has been utilized in studies examining the regulation of CXCL2 expression during Mycobacterium tuberculosis infection. Research has shown that CXCL2 is modulated through the Hippo signaling pathway, which is activated during such infections, highlighting its role in directing immune responses and inflammation ().

Is this antibody effective for neutralizing CXCL2 in vivo?

The CXCL2/GRO beta/MIP-2 antibody has been demonstrated to effectively neutralize CXCL2 in vivo, as evidenced by studies where its administration significantly reduced inflammation and improved outcomes in models of Bacillus endophthalmitis ().

What applications has this antibody been shown to work in?

The CXCL2/GRO beta/MIP-2 antibody has been shown to work in various applications, including Western blotting, immunocytochemistry, and immunohistochemistry. It has been used to detect CXCL2 in different experimental contexts, such as in studies of tissue remodeling during tooth eruption and in inflammatory responses in the respiratory system (, ).

How specific is this antibody for mouse CXCL2, and does it cross-react with other chemokines?

This antibody is highly specific for mouse CXCL2/MIP-2, with less than 10% cross-reactivity observed with recombinant human GRO beta and other mouse chemokines such as GRO gamma, KC, and LIX in direct ELISAs and Western blots. This specificity makes it a reliable tool for studying mouse models ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Comparative analysis of senolytic drugs reveals mitochondrial determinants of efficacy and resistanceNature AgingJanuary 29, 2026Masahiro Wakita, Koyu Ito, Kaho Fujii, Dai Sakamoto, Takumi Mikawa, Sho Sugawara, Xiangyu Zhou, Jeong Hoon Park, Hideka Miyagawa, Daisuke Motooka, et al.incubation with primary antibodies (XRCC4, 1:200 dilution, sc-271087, Santa Cruz; CXCL2, 1:50 dilution, AF-452-NA, R&D Systems; GROα/β/γ, 1:50 dilution, sc-365870, Santa Cruz; CXCL5, 1:50 dilution, AF254, R&D Systems;

(2) Spatially separated epithelium-associated and lamina propria neutrophils present distinct functional identities in the inflamed colon mucosaMucosal immunologyJune 1, 2025Yalom LK, Herrnreiter CJ, Bui TM, Lockhart J, Piccolo EB, Ren X, Wei C, Serdiukova A, Thorp EB, Dulai PS, et al.(554723), cells were stained for intracellular markers such as TNFα (MP6-XT22), pro-IL1β (NJTEN3), CXCL2 (AF452-NA, R&D Systems, Minneapolis, MN) overnight at 4 °C (1:100 each). Secondary antibody staining (1:500) for

(3) Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidationNature communicationsAugust 22, 2024Chung KP, Cheng CN, Chen YJ, Hsu CL, Huang YL, Hsieh MS, Kuo HC, Lin YT, Juan YH, Nakahira K, et al.

(4) IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophilsCell reportsJune 27, 2023Bénard A, Mittelstädt A, Klösch B, Glanz K, Müller J, Schoen J, Nüse B, Brunner M, Naschberger E, Stürzl M, et al.

(5) Dose-dependent pleiotropic role of neutrophils during acetaminophen-induced liver injury in male and female miceArchives of ToxicologyMay 1, 2023Nga T. Nguyen, David S. Umbaugh, Sawyer Smith, Olamide B. Adelusi, Giselle Sanchez-Guerrero, Anup Ramachandran, Hartmut Jaeschkeseparate experiment, mice were treated with a Cxcl2 neutralizing antibody (20 μg, i.p., R&D Systems, AF-452-NA) or isotype-matched IgG (R&D Systems, AB-108-C) 2 h after APAP; animals were euthanized at 24 h. Biochemical

(6) Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortexNature neuroscienceJuly 1, 2022Dong X, Zhang Q, Yu X, Wang D, Ma J, Ma J, Shi SH.

(7) C-X-C Chemokines Influence Intraocular Inflammation During Bacillus EndophthalmitisInvestigative ophthalmology & visual scienceNovember 1, 2021Mursalin MH, Coburn PS, Miller FC, Livingston ET, Astley R, Callegan MC.following treatment groups: anti-CXCL2 monoclonal antibody (anti-CXCL2; 250 ng/0.5 µL anti-CXCL2/MIP-2 IgG, AF-452-NA clone 48415; R&D Systems), anti-CXCL10 monoclonal antibody (anti-CXCL10; 250 ng/0.5 µL anti-CXCL10/IP-10

(8) Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination TherapyInternational journal of molecular sciencesOctober 16, 2021Urbantat RM, Jelgersma C, Brandenburg S, Nieminen-Kelhä M, Kremenetskaia I, Zollfrank J, Mueller S, Rubarth K, Koch A, Vajkoczy P, et al.endothelial cells (rat anti-CD31, BD Pharmingen (550274); 1:50, 16 h, 4 °C); CXCL2 (goat, R&D Systems (AF-452-NA), Minneapolis, MN, USA; 1:20, 16 h, 4 °C), and CXCR2 (rabbit, abcam (ab14935), Cambridge, UK; 1:200,

(9) Role of Innate Inflammation in the Regulation of Tissue Remodeling during Tooth EruptionDentistry journalJanuary 12, 2021Makino Y, Fujikawa K, Matsuki-Fukushima M, Inoue S, Nakamura M.monoclonal antibodies, as well as goat anti-mouse macrophage inflammatory protein-2 (MIP-2/CXCL2) (AF-452-NA, R&D Systems, Minneapolis, MN, USA) and anti-mouse keratinocyte-derived chemokine (KC/CXCL1) polyclonal

(10) Cryptotanshinone enhances wound healing in type 2 diabetes with modulatory effects on inflammation, angiogenesis and extracellular matrix remodellingPharm BiolDecember 1, 2020Song M, Chen L, Zhang L, Li C, Coffie JW, Fang Z, Zhang L, Wang S, Gao X, Wang H.

(11) Lack of whey acidic protein (WAP) four-disulfide core domain protease inhibitor 2 (WFDC2) causes neonatal death from respiratory failure in miceDisease models & mechanismsNovember 12, 2019Nakajima K, Ono M, Radović U, Dizdarević S, Tomizawa SI, Kuroha K, Nagamatsu G, Hoshi I, Matsunaga R, Shirakawa T, et al.

(12) Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancerJournal of hematology & oncologyJanuary 25, 2019Hwang WL, Lan HY, Cheng WC, Huang SC, Yang MH.CXCL1 neutralizing antibody (AF-453-NA, R&D Systems Inc.), 5 μg/ml of CXCL2 neutralizing antibody (AF-452-NA, R&D System Inc.), or 10 μg/ml of control IgG antibody (AB-108-C, R&D System Inc.) for 6 h. Cells in

(13) Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitmentJournal of leukocyte biologyJanuary 1, 2019Zhou J, Li J, Yu Y, Liu Y, Li H, Liu Y, Wang J, Zhang L, Lu X, Chen Z, et al.

(14) Cellular content plays a crucial role in Non-typeable Haemophilus influenzae infection of preinflamed Junbo mouse middle earCellular microbiologyJanuary 1, 2019Vikhe PP, Purnell T, Brown SDM, Hood DW.antibodies against respective chemokines obtained from the R&D systems™ (CCL2‐AF479‐NA, CCL3‐MAB450, CXCL2‐AF452‐NA, CXCR2–MAB2164) were added and incubated for 2 hr at room temperature with shaking. The wells were washed

(15) Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil DiapedesisImmunityDecember 18, 2018Girbl T, Lenn T, Perez L, Rolas L, Barkaway A, Thiriot A, Del Fresno C, Lynam E, Hub E, Thelen M, et al.(polyclonal),R&D Systems,Cat#AF-453-NA; RRID: AB_354495 Anti-mouse CXCL2 (polyclonal),R&D Systems,Cat#AF-452-NA; RRID: AB_2086326 Blocking anti-mouse CXCL5 (clone 61905),R&D Systems,Cat#MAB433; RRID: AB_2086587 Blocking

(16) An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAEJournal of neuroinflammationJuly 16, 2018Stoolman JS, Duncker PC, Huber AK, Giles DA, Washnock-Schmid JM, Soulika AM, Segal BM.

(17) Novel adherent CD11b⁺ Gr-1⁺ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironmentOncotargetFebruary 16, 2018Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, Kuchimaru T, Sakamoto T, Watanabe H, Kondoh G, et al.

(18) CXCL1 and CXCL2 Regulate NLRP3 Inflammasome Activation via G-Protein-Coupled Receptor CXCR2Journal of immunology (Baltimore, Md. : 1950)September 1, 2017Boro M, Balaji KN.Ab was obtained from Santa Cruz Biotechnology. CXCL1-neutralizing (AF-453NA) and CXCL2-neutralizing (AF-452-NA) Abs were purchased from R&D Systems. Goat IgG (02-6202) isotype control Ab was procured from Thermo Fisher Scientific

(19) Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein C expression in alveolar epithelial cellsAmerican journal of physiology. Lung cellular and molecular physiologyJune 1, 2017Puthusseri B, Marudamuthu A, Tiwari N, Fu J, Idell S, Shetty S.

(20) TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expressionScientific reportsDecember 20, 2016Inoue A, Matsumoto I, Tanaka Y, Umeda N, Takai C, Kawaguchi H, Ebe H, Yoshida H, Matsumoto Y, Segawa S, et al.

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