CXCL1/GRO alpha/KC/CINC-1 Antibody AF-453-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCXCL1/GRO alpha/KC/CINC-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-453-NA
NCBI Gene AliasesKC, SCYB1, Gro1, Scyb1, MGSA-a, Mgsa, GROa, NAP-3, N51, FSP, Fsp, gro, GRO1, MGSA
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunocytochemistry
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityMouse
TargetCXCL1/GRO alpha/KC/CINC-1
HostGoat
IsotypeIgG
NCBI Full Gene NameC-X-C motif chemokine ligand 1, chemokine (C-X-C motif) ligand 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1, Arg20-Lys96, Accession # P12850
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study neutrophil behavior in inflammatory conditions?

Yes, the CXCL1/GRO alpha/KC/CINC-1 Antibody has been shown to be effective in studies investigating neutrophil trafficking and behavior in inflamed tissues. For instance, it was utilized in research demonstrating that CXCL1 plays a crucial role in promoting aberrant neutrophil adhesion and migration in models of inflammation, particularly in aged tissues where CXCL1 levels are elevated (; ).

Is this antibody suitable for use in flow cytometry applications?

The CXCL1/GRO alpha/KC/CINC-1 Antibody is applicable for flow cytometry, as indicated by its usage in studies that analyze immune cell populations and their responses in various inflammatory contexts. This includes its role in assessing neutrophil dynamics in response to chemokine signaling (; ).

What experimental models have utilized this antibody for studying CXCL1's role in inflammation?

This antibody has been employed in various experimental models, including those investigating the effects of Mycobacterium tuberculosis on CXCL1 expression and its subsequent impact on immune responses (; ). Additionally, it has been used in studies examining the role of CXCL1 in neutrophil recruitment during respiratory infections ().

How has this antibody contributed to understanding CXCL1's function in cancer research?

The CXCL1/GRO alpha/KC/CINC-1 Antibody has been used in cancer research to elucidate the role of CXCL1 in tumor-associated macrophages and its influence on breast cancer progression. Studies have shown that CXCL1 can enhance tumor cell migration and invasion, highlighting its potential as a therapeutic target in cancer treatment ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) CXCL1-CXCR2 signaling mediates the activation of microglia in the nucleus tractus solitarii to promote pancreatic cancer-induced painBrain, behavior, and immunityJanuary 1, 2025Chen K, Ye Q, Zhang Y, Qi Z, Huang Y, Lu W, Wang X, Wang Y, Cao L, Qiu S, et al.

(2) Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissuesEMBO reportsSeptember 1, 2024Rolas L, Stein M, Barkaway A, Reglero-Real N, Sciacca E, Yaseen M, Wang H, Vazquez-Martinez L, Golding M, Blacksell IA, et al.Clone 20E3,Cell Sigaling technology,Cat #9719S Anti-mouse CXCL1 (for tissue staining),R&D Systems,Cat #AF-453-NA Anti-mouse CXCL1 (blocking experiments) clone 48415,R&D Systems,Cat #MAB453-100 Anti-mouse p21 Clone

(3) Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exerciseThe Journal of biological chemistryDecember 1, 2022Komori T, Morikawa Y.1:1000, catalog no.: AF-456-NA, R&D Systems); goat anti-CXCL1 antibody (diluted at 1:1000, catalog no.: AF-453-NA, R&D Systems); goat anti-OSMRβ antibody (diluted at 1:1000, R&D Systems); rabbit anti–phospho-STAT3

(4) Neutrophil breaching of the blood vessel pericyte layer during diapedesis requires mast cell-derived IL-17ANature communicationsNovember 17, 2022Joulia R, Guerrero-Fonseca IM, Girbl T, Coates JA, Stein M, Vázquez-Martínez L, Lynam E, Whiteford J, Schnoor M, Voehringer D, et al.

(5) Infiltration of peripheral immune cells into the olfactory bulb in a mouse model of acute nasal inflammationJournal of neuroimmunologyJuly 15, 2022Asano H, Hasegawa-Ishii S, Arae K, Obara A, Laumet G, Dantzer R, Shimada A.(C) IL-1β,goat,Abcam,1:200,ab9722,× CCL2,goat,R&D Systems,1:200,AF-479-NA,× CXCL1,goat,R&D Systems,1:100,AF-453-NA,× E-Selectin,chicken,R&D Systems,1:100,AF575,〇 (T) VCAM-1,rabbit,Abcam,1:500,ab134047,〇 (T) ICAM-1,goat,R&D Systems

(6) Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortexNature neuroscienceJuly 1, 2022Dong X, Zhang Q, Yu X, Wang D, Ma J, Ma J, Shi SH.

(7) Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damageImmunityJuly 13, 2021Barkaway A, Rolas L, Joulia R, Bodkin J, Lenn T, Owen-Woods C, Reglero-Real N, Stein M, Vázquez-Martínez L, Girbl T, et al.D13A2),Cell Signaling Technology,Cat# 3074T; RRID: AB_1147633 Anti-mouse CXCL1 (polyclonal),R&D systems,Cat# AF-453-NA; RRID: AB_354495 Anti-mouse CXCR2 Alexa Fluor 647 (clone SA044G4),Biolegend,Cat# 149305; RRID: AB_2565693 Anti-mouse

(8) Role of Innate Inflammation in the Regulation of Tissue Remodeling during Tooth EruptionDentistry journalJanuary 12, 2021Makino Y, Fujikawa K, Matsuki-Fukushima M, Inoue S, Nakamura M.Minneapolis, MN, USA) and anti-mouse keratinocyte-derived chemokine (KC/CXCL1) polyclonal antibodies (AF-453-NA, R&D Systems). Rat antimouse ICAM-1 monoclonal antibody was used to identify ICAM-1-expressing cells

(9) Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in miceToxicology reportsJanuary 1, 2021Shimada A, Hasegawa-Ishii S.

(10) Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated MacrophagesCancersNovember 26, 2020Wang YY, Chen HD, Lo S, Chen YK, Huang YC, Hu SC, Hsieh YC, Hung AC, Hou MF, Yuan SF.Laboratory Animal Center, Taiwan) through the tail-vein. After one week, CXCL1 blocking antibody (10 mg/kg) (AF-453-NA, R&D Systems) was injected intraperitoneally twice per week. The bioluminescent signal from Luc-MDA-MB-231 cells

(11) Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancerJournal of hematology & oncologyJanuary 25, 2019Hwang WL, Lan HY, Cheng WC, Huang SC, Yang MH.device and lower chamber containing 600 μl of SDCSC medium plus 5 μg/ml CXCL1 neutralizing antibody (AF-453-NA, R&D Systems Inc.), 5 μg/ml of CXCL2 neutralizing antibody (AF-452-NA, R&D System Inc.), or 10 μg/ml

(12) Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil DiapedesisImmunityDecember 18, 2018Girbl T, Lenn T, Perez L, Rolas L, Barkaway A, Thiriot A, Del Fresno C, Lynam E, Hub E, Thelen M, et al.(clone 40605),R&D Systems,Cat#MAB452; RRID: AB_2230058 Anti-mouse CXCL1 (polyclonal),R&D Systems,Cat#AF-453-NA; RRID: AB_354495 Anti-mouse CXCL2 (polyclonal),R&D Systems,Cat#AF-452-NA; RRID: AB_2086326 Blocking

(13) An IFNγ/CXCL2 regulatory pathway determines lesion localization during EAEJournal of neuroinflammationJuly 16, 2018Stoolman JS, Duncker PC, Huber AK, Giles DA, Washnock-Schmid JM, Soulika AM, Segal BM.

(14) CXCL1 and CXCL2 Regulate NLRP3 Inflammasome Activation via G-Protein-Coupled Receptor CXCR2Journal of immunology (Baltimore, Md. : 1950)September 1, 2017Boro M, Balaji KN.Merck-Millipore and anti-NLRP3 (66846) Ab was obtained from Santa Cruz Biotechnology. CXCL1-neutralizing (AF-453NA) and CXCL2-neutralizing (AF-452-NA) Abs were purchased from R&D Systems. Goat IgG (02-6202) isotype

(15) Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responsesScientific reportsNovember 24, 2016Boro M, Singh V, Balaji KN.anti MYD88 (4283) antibodies were purchased from Cell Signaling Technology (USA). CXCL1 neutralizing (AF-453-NA) and CXCL2 neutralizing (AF-452-NA) antibodies were purchased from R & D Systems Inc. (USA). Goat IgG

(16) Inducible Expression of CXCL1 within the Central Nervous System Amplifies Viral-Induced DemyelinationJournal of immunology (Baltimore, Md. : 1950)February 15, 2016Marro BS, Grist JJ, Lane TE.

(17) Astrocytes Are Primed by Chronic Neurodegeneration to Produce Exaggerated Chemokine and Cell Infiltration Responses to Acute Stimulation with the Cytokines IL-1β and TNF-αThe Journal of neuroscience : the official journal of the Society for NeuroscienceJune 3, 2015Hennessy E, Griffin ÉW, Cunningham C.CCL2, 1/100, part 840288 of DY479 (R&D Systems); GFAP, 1/2000, Z0334 (Dako Cytomation); CXCL1, 1/50, AF-453-NA (R&D Systems); CD68, 1/50, MCA1957, (AbDSerotec); CD3, 1/250, A0452 (Dako Cytomation); Pu.1, 1/400,

(18) P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathyCell researchJune 1, 2015Wang H, Hong LJ, Huang JY, Jiang Q, Tao RR, Tan C, Lu NN, Wang CK, Ahmed MM, Lu YM, et al.

(19) Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancyThe Journal of biological chemistryJanuary 23, 2015Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce JS, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K.

(20) CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-βInternational journal of cancerMarch 15, 2014Jablonska J, Wu CF, Andzinski L, Leschner S, Weiss S.

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