PD-1 Antibody AF1086 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemPD-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF1086
ApplicationsBioassay,ELISA Development,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Microarray,Blockade of Receptor-ligand Interaction,Neutralization,ELISA Capture (Matched Antibody Pair),Immunocytochemistry,CyTOF-ready,Functional Assay,Surface Plasmon Resonance (SPR),Dual RNAscope ISH-IHC Compatible
TargetPD-1
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityChinese Hamster,Feline,Human,Mouse,Primate,Primate - Cercocebus atys (Sooty Mangabey),Primate - Macaca mulatta (Rhesus Macaque)
HostGoat
IsotypeIgG
Conjugate/TagUnconjugated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesCD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1
NCBI Full Gene Nameprogrammed cell death 1
PurityAntigen Affinity-purified
ConcentrationLYOPH
ImmunogenMouse myeloma cell line NS0-derived recombinant human PD-1, Leu25-Gln167, Accession # Q8IX89
ClonalityPolyclonal
Quantity100 ug (also 25 ug)

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD-1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA-4 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this PD-1 antibody be used in immunohistochemistry for studying tumor microenvironments?

Yes, the PD-1 antibody has been utilized in immunohistochemical studies, as indicated by its application in detecting PD-1 expression in various tissues, including those involved in tumorigenesis. For instance, it has been employed to analyze PD-1 expression in human ovarian and fallopian tube specimens, suggesting its relevance in understanding immune modulation in cancer contexts.

How effective is this antibody in blocking PD-1 interactions in functional assays?

The PD-1 antibody has been shown to effectively block PD-1/PD-L1 interactions in functional assays. In studies, it was used to inhibit PD-1 signaling, which enhanced the efficacy of chemotherapeutic agents like doxorubicin in mouse models of breast cancer, indicating its potential in therapeutic applications.

Is this antibody suitable for flow cytometry applications?

Yes, the PD-1 antibody is suitable for flow cytometry applications. It has been cited in studies where it was used to analyze PD-1 expression on T cells, demonstrating its utility in characterizing immune cell populations in various experimental setups.

What species can this PD-1 antibody detect, and how specific is it?

This PD-1 antibody can detect PD-1 in multiple species, including humans, mice, non-human primates, and felines. It exhibits high specificity, with less than 2% cross-reactivity with recombinant mouse PD-1 and minimal cross-reactivity with other related proteins, making it a reliable choice for studies across different species.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Angiogenic and immune predictors of neoadjuvant axitinib response in renal cell carcinoma with venous tumour thrombusNat CommunApril 28, 2025Rebecca Wray, Hania Paverd, Ines Machado, Johanna Barbieri, Farhana Easita, Abigail R. Edwards, Ferdia A. Gallagher, Iosif A. Mendichovszky, Thomas J. Mitchell, Maike de la Roche, et al.Abcam ab92742), CD8 (SP16, Invitrogen MA5-14548), Granzyme B (Leica NCL-L-GRAN-B), PD-1 (R&D Systems AF1086), CD4 (EPR6855, Abcam ab133616), FOXP3 (236A/E7, Abcam ab20034), CA9 (R&D Systems AF2188), CD3 (D7A6E,

(2) Role of stromal PD-L1 expression in colorectal liver metastasisBMC CancerJanuary 17, 2024Chie Takasu, Yuji Morine, Kozo Yoshikawa, Toshihiro Nakao, Takuya Tokunaga, Masaaki Nishi, Hideya Kashihara, Yuma Wada, Toshiaki Yoshimoto, Mitsuo Shimadaprimary antibody at 4 °C overnight. Primary antibodies against the following proteins were used: PD-1 (AF1086, 1:40; R&D Systems, Minneapolis, MN, USA), PD-L1 (ab174838, 1:100; Abcam, Cambridge, UK), CD3 (ab5690,

(3) Comparison of Follicular Helper T-Cell Markers with the Expression of the Follicular Homing Marker CXCR5 in Peripheral T-Cell Lymphomas-A Reappraisal of Follicular Helper T-Cell LymphomasInternational journal of molecular sciencesDecember 28, 2023Krenács L, Krenács D, Borbényi Z, Tóth E, Nagy A, Piukovics K, Bagdi E.

(4) Modulation of immune checkpoint regulators in interferon γ induced urothelial carcinoma and activated T-lymphocyte cells by cytostaticsGenes and immunityJune 1, 2023Hänze J, Schulte-Herbrüggen J, Hofmann R, Hegele A.TTCATGAGGTAGTCAGTCAGGTCCC. The antibodies were as follows: PD-L1 #13684 (Cell signaling), PD-1, host goat (AF1086, R&D Systems); (LDHA #3558, Cell signaling). Data were analysed with MS-Office Excel and Graphpad Prism

(5) Expression and T cell regulatory action of the PD‐1 immune checkpoint in the ovary and fallopian tubeAmerican Journal of Reproductive ImmunologyMarch 1, 2023Joshua Johnson, So‐Youn Kim, Peter Ka Sam, Rengasamy Asokan, Evelyn Llerena Cari, Elise S. Bales, Thanh‐Ha Luu, Lauren Perez, Amanda N. Kallen, Liesl Nel‐Themaat, et al.Target,Supplier,Catalog # HUMAN,, PD-1,Cell Signaling Technology,#86163 PD-1†,R&D Systems,#AF1086 PD1 (phospho Y248),abcam,#ab206378 PD-L1,ThermoFisher,#PA5–20343 PD-L2,BIO-RAD,AHP1704 MOUSE,, Pd-1,"ProSci,

(6) Exosomes in malignant pleural effusion from lung cancer patients impaired the cytotoxicity of double-negative T cellsTranslational oncologyJanuary 1, 2023Wu J, Zhu R, Wang Z, Chen X, Xu T, Liu Y, Song M, Jiang J, Ma Q, Chen Z, et al.some assays, DNT cells were cocultured with anti-human PD-1 antibody (0.1, 1 μg/ml, R&D Systems, cat# AF1086) for 24 h before seeding into 96-well plates. Then, 20 μl of CCK-8 reagent was added to each well and

(7) Cancer cell intrinsic TIM-3 induces glioblastoma progressioniScienceNovember 18, 2022Guo Q, Shen S, Guan G, Zhu C, Zou C, Cao J, Cheng W, Xu X, Yu J, Lin Z, et al.AB_2687655 PDL2(D7U8C),Cell Signaling Technology,82723T IDO1,Proteintech,13268 PD-1,R and D Systems,AF1086; RRID: AB_354588 MMP2,Abcam,ab37150; RRID: AB_881512 IL6,Abcam,ab6672; RRID: AB_2127460 IL6,R & D

(8) Escherichia coli-Specific CXCL13-Producing TFH Are Associated with Clinical Efficacy of Neoadjuvant PD-1 Blockade against Muscle-Invasive Bladder CancerCancer discoveryOctober 5, 2022Goubet AG, Lordello L, Alves Costa Silva C, Peguillet I, Gazzano M, Mbogning-Fonkou MD, Thelemaque C, Lebacle C, Thibault C, Audenet F, et al.

(9) Cellular and soluble immune checkpoint signaling forms PD-L1 and PD-1 in renal tumor tissue and in bloodCancer immunology, immunotherapy : CIIOctober 1, 2022Keber CU, Derigs M, Schultz C, Wegner M, Lingelbach S, Wischmann V, Hofmann R, Denkert C, Hegele A, Hänze J.24 h. The antibodies were as follows: PD-L1, host rabbit (#13,684, Cell signaling); PD-1, host goat (AF1086, R&D Systems); cytoplasmic β-actin, host mouse (#MAK6019 Linaris GmbH). Then, the respective secondary

(10) Ex vivo-expanded human CD19(+)TIM-1(+) regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axisNature communicationsJune 3, 2022Shankar S, Stolp J, Juvet SC, Beckett J, Macklin PS, Issa F, Hester J, Wood KJ.

(11) Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under ImmunotherapyCancersJanuary 21, 2022Rafei-Shamsabadi D, Lehr S, Behrens M, Meiss F.dilution of 1:100) and PD-1 staining with a polyclonal goat antibody (R&D systems; Catalog Number: AF1086; dilution 1:100). Additional antibodies (Ab) for IHC included anti-CD4 Ab (Zytomed, catalog number:

(12) Tumor-endogenous PD-1 promotes cell proliferation and predicts poor survival in non-small cell lung cancerTranslational cancer researchJanuary 1, 2022Gan F, Zhang C, Xia L, Deng S.respectively. We then incubated the tissue sections with anti-PD-1 antibody (1:200; R and D Systems Cat# AF1086, RRID:AB_354588) at 4 °C overnight and anti-rabbit secondary antibody (1:1,000, ZSGB-Bio Cat# PV-6001,

(13) Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancerCancer communications (London, England)January 1, 2022Cheng B, Ding K, Chen P, Ji J, Luo T, Guo X, Qiu W, Ma C, Meng X, Wang J, et al.patients, PBMCs extracted from patients were pretreated with 10 μg/mL SHR‐1701, 10 μg/mL anti‐PD‐1 Abs (#AF1086, R&D systems, Minneapolis, Minnesota, USA) or 1 μmol/L TGF‐βR inhibitors (#S1476, Selleck, Houston,

(14) Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS ProtocolInternational journal of molecular sciencesDecember 25, 2021von Schoenfeld A, Bronsert P, Poc M, Fuller A, Filby A, Kraft S, Kurowski K, Sörensen K, Huber J, Pfeiffer J, et al.9 PAX5,DAK-Pax5,Mouse,RTU,Dako(IR65061-2),20 min TRIS pH 6.1 PD-1,Polyclonal,Goat,1:100,R&D Systems(AF1086),5 min HPC RBPJ,Polyclonal,Rabbit,1:50,Thermo Fisher(PA5-35187),2 min HPC Primary antibodies used,

(15) Gut germinal center regeneration and enhanced antiviral immunity by mesenchymal stem/stromal cells in SIV infectionJCI insightJune 22, 2021Weber MG, Walters-Laird CJ, Kol A, Santos Rocha C, Hirao LA, Mende A, Balan B, Arredondo J, Elizaldi SR, Iyer SS, et al.blocking antibody for 1 hour. Primary antibodies CD3 (1:200) (DAKO, A0452) and PD-1 (1:50) (R&D Systems, AF1086) were applied overnight at 4°C. Secondary antibodies PD-1, donkey, anti-goat, Alexa Fluor 647 (Invitrogen,

(16) The role of the immunoescape in colorectal cancer liver metastasisPloS oneJanuary 1, 2021Takasu C, Yamashita S, Morine Y, Yoshikawa K, Tokunaga T, Nishi M, Kashihara H, Yoshimoto T, Shimada M.4°C. The primary antibodies and dilutions were as follows: mouse monoclonal antibody against PD-1 (AF1086, 1:40; R&D Systems, Minneapolis, USA), rabbit monoclonal antibody against PD-L1 (ab174838, 1:100; Abcam,

(17) Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signalingRedox biologyJanuary 1, 2021Glorieux C, Xia X, He YQ, Hu Y, Cremer K, Robert A, Liu J, Wang F, Ling J, Chiao PJ, et al.

(18) The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/β-catenin signaling pathwayCellular & molecular immunologyNovember 1, 2020Deng R, Zuo C, Li Y, Xue B, Xun Z, Guo Y, Wang X, Xu Y, Tian R, Chen S, et al.interaction: human PD-L1 antibody (AF156, R&D Systems, Minneapolis, MN, USA), human PD-1 antibody (AF1086, R&D Systems), and normal goat IgG control (AB-108-C, R&D Systems). The following antibodies were obtained

(19) Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathologyNature protocolsMay 1, 2020Bucur O, Fu F, Calderon M, Mylvaganam GH, Ly NL, Day J, Watkin S, Walker BD, Boyden ES, Zhao Y.Aldrich,ABC137,AB_2811109 CD8,Rabbit,poly,Abcam,ab85792,AB_10674324 P24,Mouse,Mono,Dako,M0857,AB_2335686 PD-1,Goat,Poly,R & D systems,AF1086,AB_354588 Hsp 60,Mouse,Mono,Abcam,ab59457,AB_2121285 Examples of primary antibodies validated in

(20) Concordance of PD-1 and PD-L1 (B7-H1) in paired primary and metastatic clear cell renal cell carcinomaCancer medicineFebruary 1, 2020Eckel-Passow JE, Ho TH, Serie DJ, Cheville JC, Houston Thompson R, Costello BA, Dong H, Kwon ED, Leibovich BC, Parker AS.

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PD-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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PD-1 Antibody from R&D Systems, a Bio-Techne Brand

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