Galectin-8 Antibody AF1305 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGalectin-8 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1305
NCBI Gene AliasesPo66-CBP, Gal-8, PCTA-1, PCTA1
ApplicationsELISA Development (Detection),Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry,ELISA Detection
TargetGalectin-8
ReactivityComplex Species Category,Human,Mouse
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
IsotypeIgG
Quantity100 ug (also 25 ug)
NCBI Full Gene Namegalectin 8
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human Galectin-8, Met1-Trp317, Accession # O00214
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Galectin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) rhGalectin-1 and rhGalectin-3 is observed, and less than 1% cross-reactivity with rhGalectin-2, rhGalectin-4 and rhGalectin-7 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study the role of Galectin-8 in autophagy-related processes?

Yes, the Galectin-8 antibody has been shown to play a significant role in autophagy. For instance, it is involved in detecting damaged endomembranes and activating autophagy through the recruitment of the cargo receptor NDP52, as demonstrated in studies focusing on tau aggregation and cellular defenses against cytosolic microorganisms ().

Is the Galectin-8 antibody effective for use in immunofluorescence applications?

The Galectin-8 antibody has been utilized successfully in immunofluorescence assays, as indicated by its application in various studies, including those examining the interactions of Mycobacterium tuberculosis with host cells (, ).

What species can this antibody detect, and how specific is it?

The Galectin-8 antibody is reactive to both human and mouse species. It has been shown to specifically detect human Galectin-8 with less than 5% cross-reactivity with other galectins, such as Galectin-1 and Galectin-3, and minimal cross-reactivity with Galectin-2, Galectin-4, and Galectin-7 ().

Are there any notable applications of this antibody in recent research?

Yes, the Galectin-8 antibody has been cited in multiple studies, including its role in the immune response to Toxoplasma gondii, where it was used to investigate the ubiquitination and destruction of the parasite in human cells (). Additionally, it has been implicated in the selective autophagy processes that protect against tau aggregation in neurodegenerative diseases ().

R&D Systems, a Bio-Techne Brand

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Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Ca²⁺ leakage is a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repairThe EMBO JournalMarch 20, 2026Di Chen, Antony Fearns, Christopher J Peddie, Maximiliano G Gutierrezfollowing antibodies were used: Galectin-3 (BioLegend, Cat# 125410), Galectin-8 (R&D Systems, Cat# AF1305), LC3B (MBL, Cat# PM036), LC3B (MBL, Cat# M152-3), ATP6V1D (Abcam, Cat# ab157458), CHMP4B (Ptoteintech,

(2) Mycobacterium tuberculosis phagosome Ca(2+) leakage triggers multimembrane ATG8/LC3 lipidation to restrict damage in human macrophagesScience advancesMarch 28, 2025Chen D, Fearns A, Gutierrez MG.or 18-well plate. The antibodies used are as follows: GAL3 (BioLegend, 125410), GAL8 (R&D Systems, AF1305), LC3B (Sigma-Aldrich, L7543), p62 (CST, 88588), ATP6V1D (Abcam, ab157458), and CD63 (Abcam, ab217345). Western blot analysis

(3) Selective autophagy impedes KSHV entry after recruiting the membrane damage sensor galectin-8 to virus-containing endosomesCell reportsDecember 24, 2024Schmidt KW, Montespan C, Thompson D, Lucas MS, Ligeon LA, Wodrich H, Hahn AS, Greber UF, Münz C.

(4) Human GBP1 facilitates the rupture of the Legionella-containing vacuole and inflammasome activationmBioOctober 31, 2023Bass AR, Egan MS, Alexander-Floyd J, Lopes Fischer N, Doerner J, Shin S.staining experiments, goat anti-galectin-8 (1:200 dilution in primary hMDMs and 1:100 in THP-1 cells; AF1305; R&D Systems) and donkey anti-goat IgG conjugated to Alexa Fluor 488 (1:4,000 dilution; A11055; Invitrogen).

(5) TECPR1 conjugates LC3 to damaged endomembranes upon detection of sphingomyelin exposureThe EMBO journalSeptember 4, 2023Boyle KB, Ellison CJ, Elliott PR, Schuschnig M, Grimes K, Dionne MS, Sasakawa C, Munro S, Martens S, Randow F.blocked in PBS/2% BSA for 30 min. Samples were stained with primary antibody (1:50 for anti‐Galectin‐8 [AF1305 R&D Systems], 1:200 for anti‐WIPI2 [ab105459, Abcam], 1:200 anti‐ATG5 [A0731, Sigma]) for 1 h, rinsed

(6) mTOR-regulated mitochondrial metabolism limits mycobacterium-induced cytotoxicityCellSeptember 29, 2022Pagán AJ, Lee LJ, Edwards-Hicks J, Moens CB, Tobin DM, Busch-Nentwich EM, Pearce EL, Ramakrishnan L.Conjugate),Cell Signaling Technology,Cat# 5548;RRID:AB_10707322 Goat IgG anti-human Galectin-8,R&D Systems,Cat# AF1305;RRID:AB_2137229 "Donkey anti-goat IgG (H+L), Alexa Fluor 488",Thermo Fisher Scientific,Cat# A-11055;

(7) TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escapePLoS pathogensJuly 1, 2022Pied N, Daussy CF, Denis Z, Ragues J, Faure M, Iggo R, Tschan MP, Roger B, Rayne F, Wodrich H.3504",1/1000, Actin,"Merck, ab1501",1/2000, mRFP,"Chromotek, clone 5F8",1/1000, Galectin8,"R&D system, AF1305",,1/200 Galectin8,"Abcam, ab42879",1/500, NDP52,"Abcam, ab68588",1/1000,1/200 Tax1BP1,"Cell Signaling

(8) Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated DeathSSRN Electronic JournalApril 2, 2022Daniel Fisch, Barbara Clough, Marie-Charlotte Domart, Vesela Encheva, Hironori Bando, Ambrosius P. Snijders, Lucy M. Collinson, Masahiro Yamamoto, Avinash R. Shenoy, Eva Frickelmonoclonal anti-Flag M2 Sigma Cat#F3165; RRID:AB_259529 Goat polyclonal anti-Galectin-8 R&D Systems Cat#AF1305; RRID:AB_2137229 Mouse monoclonal anti-GBP1 Home-made (Frickel lab) N/A Rabbit polyclonal anti-mCherry Abcam Cat#ab167453;

(9) The C terminus of the mycobacterium ESX-1 secretion system substrate ESAT-6 is required for phagosomal membrane damage and virulenceProceedings of the National Academy of Sciences of the United States of AmericaMarch 15, 2022Osman MM, Shanahan JK, Chu F, Takaki KK, Pinckert ML, Pagán AJ, Brosch R, Conrad WH, Ramakrishnan L.30 min at room temperature and then stained with goat anti-human galectin-8 antibody (R&D Systems, AF1305) diluted in PB solution overnight at 4 °C. Cells were then washed three times with PBS and stained with

(10) Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagyeLifeSeptember 29, 2021Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW.RRID:AB_627658,"IF (1:300), WB (1:1000)" Antibody,"Human Galectin-8/LGALS8 Antibody, (goat polyclonal)",R&D Systems,AF1305; RRID:AB_2137229,"IF (1:300), WB (1:1000)" Antibody,"LC3B D11 (Rabbit mAb, monoclonal)",Cell Signaling Technology

(11) Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infectionNatureJune 1, 2021Otten EG, Werner E, Crespillo-Casado A, Boyle KB, Dharamdasani V, Pathe C, Santhanam B, Randow F.(1:1000, JL8, Clontech, 632381), α-LPS (1:100, BioRad, 8210-0407), α-Galectin 8 (1:100, R&D Systems, AF1305), α-M1 (1:1000, 1F11/3F5/Y102L, GenenTech) and α-LC3 (1:100, Cosmo Bio, CTB-LC3-2-IC). Secondary antibodies

(12) Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestylePLoS pathogensNovember 1, 2020Badgujar DC, Anil A, Green AE, Surve MV, Madhavan S, Beckett A, Prior IA, Godsora BK, Patil SB, More PK, et al.immunofluorescence microscopy were anti-Transferrin (PA3913, Pierce), anti-FITC (31242, Invitrogen), anti-Gal8 (AF1305, R&D Systems), anti-Ubiquitin (BML-PW8100, Enzo Life Sciences), anti-LC3 (4108, CST) and anti-LAMP1

(13) LRRK2 activation controls the repair of damaged endomembranes in macrophagesThe EMBO journalSeptember 15, 2020Herbst S, Campbell P, Harvey J, Bernard EM, Papayannopoulos V, Wood NW, Morris HR, Gutierrez MG.from BioLegend; anti‐CHMP4B (13683‐1AP) from ProteinTech; anti‐LC3B (PM036) from MBL; anti‐Galectin‐8 (AF1305) from R&D Systems; and anti‐LAMP‐1 for immunofluorescence (1D4B) from DSHB and anti‐K63 Ubiquitin (05‐1308)

(14) Human GBP1 Differentially Targets Salmonella and Toxoplasma to License Recognition of Microbial Ligands and Caspase-Mediated DeathCell reportsAugust 11, 2020Fisch D, Clough B, Domart MC, Encheva V, Bando H, Snijders AP, Collinson LM, Yamamoto M, Shenoy AR, Frickel EM.monoclonal anti-Flag M2,Sigma,Cat#F3165; RRID:AB_259529 Goat polyclonal anti-Galectin-8,R&D Systems,Cat#AF1305; RRID:AB_2137229 Mouse monoclonal anti-GBP1,Home-made (Frickel lab),N/A Rabbit polyclonal anti-mCherry,Abcam,Cat#ab167453;

(15) Mycobacterium marinum phthiocerol dimycocerosates enhance macrophage phagosomal permeabilization and membrane damagePloS oneJanuary 1, 2020Osman MM, Pagán AJ, Shanahan JK, Ramakrishnan L.minutes at room temperature, and then stained with goat anti-human galectin-8 antibody (R&D Systems, AF1305) diluted in PB solution overnight at 4˚C. Cells were then washed three times with PBS and stained with AlexaFluor488-conjugated

(16) Human trophoblast requires galectin-3 for cell migration and invasionScientific reportsFebruary 14, 2019Bojić-Trbojević Ž, Jovanović Krivokuća M, Vilotić A, Kolundžić N, Stefanoska I, Zetterberg F, Nilsson UJ, Leffler H, Vićovac L.recombinant human galectin-8 (rhgalectin-8), goat anti-galectin-3 (AF1154) and goat anti-galectin-8 (AF1305) antibodies were from R&D Systems (Abingdon UK). The mutant form of galectin-1 in which all six cysteine

(17) Heterogeneity in pneumolysin expression governs the fate of Streptococcus pneumoniae during blood-brain barrier traffickingPLoS pathogensJuly 1, 2018Surve MV, Bhutda S, Datey A, Anil A, Rawat S, Pushpakaran A, Singh D, Kim KS, Chakravortty D, Banerjee A.used in this study were against; LAMP1 (Cell Signaling Technology, D2D11), Galectin 8 (R & D Systems, AF1305), NDP52 (Abcam, ab68588), Pneumolysin (Santacruz Biotechnology, sc-80500; Abcam, ab71811), GAPDH (Millipore,

(18) Galectin-8-mediated selective autophagy protects against seeded tau aggregationThe Journal of biological chemistryFebruary 16, 2018Falcon B, Noad J, McMahon H, Randow F, Goedert M.Proteintech Group; anti-ubiquitin P4D1 (sc-8017) was from Santa Cruz Biotechnology; and anti–galectin-8 (AF1305) was from R&D Systems. Horseradish peroxidase (HRP)–conjugated secondary antibodies were from Bio-Rad.

(19) Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cellsBMC biologyJanuary 4, 2018Lerner TR, Queval CJ, Fearns A, Repnik U, Griffiths G, Gutierrez MG.hours. Antibodies The following primary antibodies were used in this study: goat anti-Galectin 8 (R&D Systems, AF1305; AB_2137229; 1:250 dilution), rabbit anti-CtsD (provided by Andrej Hasilik; 1:100 dilution), mouse anti-ubiquitin

(20) GBPs Inhibit Motility of Shigella flexneri but Are Targeted for Degradation by the Bacterial Ubiquitin Ligase IpaH9.8Cell host & microbeOctober 11, 2017Wandel MP, Pathe C, Werner EI, Ellison CJ, Boyle KB, von der Malsburg A, Rohde J, Randow F.monoclonal,Santa Cruz Biotechnology,Cat# sc-56; RRID: AB_628110 Galectin-8 goat polyclonal,R&D Systems,Cat# AF1305; RRID: AB_2137229 GBP1 rabbit polyclonal,Proteintech,Cat# 15303-1-AP; RRID: AB_2247448 GBP1 (1B1)

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