Human Fc gamma RIIIA/B (CD16) Antibody from R&D Systems, a Bio-Techne Brand

Yes, the Fc gamma RIII (CD16) antibody has been utilized in studies investigating natural killer cell activation. For instance, it was used in a study to analyze the expression of immune checkpoint markers on NK cells activated by anti-CD20 monoclonal antibodies, demonstrating its relevance in understanding NK cell biology and therapeutic responses (wang2021).

The Fc gamma RIII (CD16) antibody has been shown to work in various applications including Western blotting, flow cytometry, and immunocytochemistry. In a study, it was specifically used for Western blotting to detect CD16a in NK cell lysates, highlighting its utility in protein analysis (patel2021).

The Fc gamma RIII (CD16) antibody is specific for human Fc gamma RIIIA/B (CD16) with approximately 5% cross-reactivity with recombinant human Fc gamma RIIA and less than 2% with recombinant mouse CD16. This specificity is crucial for accurate detection in human samples.

Glycosylation of CD16 significantly impacts its binding affinity and function, particularly in antibody-dependent cell-mediated cytotoxicity (ADCC). The Fc gamma RIII (CD16) antibody can be used to investigate these effects, as studies have shown that manipulating glycan composition can enhance NK cell ADCC, which can be assessed using this antibody in various experimental setups (kremer2024).