Human PDGF Rß Antibody AF385 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman PDGF Rß Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF385
NCBI Gene AliasesIBGC4, PDGFR, PDGFR-1, KOGS, CD140B, PENTT, IMF1, PDGFR1, JTK12
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry,CyTOF-ready
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityGolden Syrian Hamster,Human,Mouse,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Primate - Macaca mulatta (Rhesus Macaque),Rabbit,Transgenic Mouse,Primate - Saimiri sciureus (Common Squirrel Monkey)
HostGoat
TargetPDGF R beta
Quantity100 ug (also 25 ug)
IsotypeIgG
NCBI Full Gene Nameplatelet derived growth factor receptor beta
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human PDGF Rß, Leu33-Phe530 (Glu241Asp), Accession # P09619
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunohistochemistry in brain tissue studies?

Yes, the PDGF R beta antibody has been utilized in immunohistochemical staining of post-mortem brain tissue, specifically in studies examining the distribution of pericytes in cerebral microvessels related to conditions like CADASIL, demonstrating its effectiveness in this application ().

Is the PDGF R beta antibody effective for detecting PDGFRβ in human tissues?

The PDGF R beta antibody has been shown to effectively detect PDGFRβ in human tissues, as evidenced by its use in various studies, including those investigating the expression of PDGFRβ in pancreatic tissues and its role in vascular remodeling (, ).

What is the recommended dilution for using this antibody in Western blotting?

The PDGF R beta antibody is typically used at a dilution of 1:200 for immunohistochemistry, which may also be applicable for Western blotting, although specific dilution recommendations for Western blots are not provided in the literature ().

Has this antibody been cited in studies related to diabetic vascular function?

Yes, the PDGF R beta antibody has been cited in research examining impaired vascular function in diabetic donor-derived vascular organoids, highlighting its relevance in studies of diabetes-related vascular changes ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) 3D Vessels-on-Chip using isogenic hiPSC-derived VSMCs reveal NOTCH3-driven alterations in brain small vessel diseaseStem Cell ReportsApril 14, 2026Marc Vila Cuenca, Theano Tsikari, Minne N. Cerfontaine, James L. Gallant, Francijna E. van den Hil, Marga J. Bouma, Kyra L. Dijkstra, Gido Gravesteijn, Antoine A.F. de Vries, Christine L. Mummery, et al.primary mouse anti-NOTCH3 antibody (clone 1E4, Millipore) or for 1 h with a primary goat anti-PDGFRβ (AF385, R&D Systems). A two-step detection system (BrightVision, ImmunoLogic, VWRKC-DPVB55HRP) was used per

(2) Differential pericyte pathology in the human retina and brain in diabetes mellitus and Alzheimer’s diseaseFrontiers in NeuroscienceFebruary 5, 2026Noëlle Bakker-van Bugnum, Aïcha A. Croes, Eva Prevaes, Cornelis J. F. van Noorden, Reinier O. Schlingemann, Ingeborg KlaassenAb11575","1:1,000" NG2,Mouse,"Merck Life Science, MAB2029",1:100 (retina)/1:200 (brain) PDGFRβ,Goat,"R&D Systems, AF385",1:100 αSMA,Mouse,"DAKO, M0851",1:500 Primary antibodies used in this study. R&D Systems (Minneapolis,

(3) Engineering scalable vascularized kidney organoids for in vivo glomerular filtration with human endothelial integrationNPJ Biomed InnovFebruary 2, 2026Murat Tekguc, Takuya Matsumoto, Lukas M. Altenburger, Kenichi Kobayashi, Ken Hiratsuka, Yuhei Higashi, Astia Rizki-Safitri, Tomoya Miyoshi, Wassim El-Jouni, M. Amin Arnaout, et al.(NPHS1),PROGEN,"GP-N2, RRID: AB_2904121" PAX2,Biolegend,"901001, RRID: AB_2565001" PDGFR-β,R&D systems,"AF385, RRID: AB_355339" NPHS2,Abcam,"ab50339, RRID: AB_882097" PODXL,R&D systems,"AF1658, RRID: AB_354920" SALL1,R&D systems

(4) Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot studyBMC NeuroscienceJanuary 12, 2026Rebecca Best Jensen, Maria Kjølhede, Jesper Just, Lee-Ann Clegg, Rikke Baek, Erik Kaadt, Betina Elfving, David C. Hess, Grethe Andersen, Malene Møller Jørgensen, et al.,R&D Systems,MAB726,"22,210" TNF RI,R&D Systems,MAB225,"16,803" Claudin 3,R&D Systems,MAB4620,"385,021" PDGF R beta,R&D Systems,AF385,- BDNF,R&D Systems,MAB648,"37,141" GFAP,R&D Systems,MAB2594,"273,807" MCP-1,R&D Systems,MAB679,"23,007" S100B,R&D Systems

(5) Modeling the metastatic tumor microenvironment: a co-culture platform of lung cancer and blood vessel organoids for drug evaluationScientific ReportsNovember 24, 2025Mingyun Lee, Yeo-Joon Yoon, Ji Yeon Lee, Mina Hwang, Sewon Park, Min Hak Lee, Sang-Won Um, Byoung Chul Cho, Jinguen Rheey, Toshiro Sato, et al.this study: CD31 (R&D systems, AF806), PDGFRβ (Cell signaling, 3169 S, Danvers, MA, USA; R&D systems, AF385, Minneapolis, MN, USA), Fibronectin (Cell signaling, 26836 S), Collagen type Ⅰ (Cell signaling, 72026

(6) Modeling the Blood-Brain Barrier Using Human-Induced Pluripotent Stem CellsMethods in Molecular BiologyNovember 21, 2025Louise A. Mesentier-Louro, Natalie Suhy, Diede Broekaart, Michael Bula, Ana C. Pereira, Joel W. BlanchardR & D Systems Cat. no. AF806).PDGFRβ (Platelet-derived growth factor receptor beta, R & D Biosystems, Cat. no. AF385).VE-cadherin (R & D Systems Cat. no. AF938). Secondary antibodies. Alexa Fluor 488, 555, 647 secondary

(7) Brain pericytes derived from human pluripotent stem cells retain vascular and phagocytic functions under hypoxiaStem CellsOctober 16, 2025Mingzi Zhang, Youbin Kim, Allison Bosworth, Julia T C W, Lina R Nih, Kassandra Kisler, Abhay P Sagare, Ruslan RustX-100. Cells were incubated overnight at 4 °C with primary antibodies against PDGFRβ (R&D Systems, AF385, 1:100, Goat) and NG2 (Sigma, AB5320, 1:500, Rabbit) diluted in PBS supplemented with 5% donkey serum

(8) Spatially patterned kidney assembloids recapitulate progenitor self-assembly and enable high-fidelity in vivo disease modelingCell Stem CellOctober 2, 2025Biao Huang, Pedro Medina, Jincan He, Zipeng Zeng, Sunghyun Kim, Janet Romo, Kari Koppitch, Chennan C. Zhang, Georgina Gyarmati, Yohan Park, et al.anti-alpha-SMA (ACTA2),Sigma-Aldrich,Cat# A5228; RRID: AB_262054 Goat Polyclonal anti-PDGFRB,R&D Systems,Cat# AF385; RRID: AB_355339 Rabbit Monoclonal anti-PDGFRB,Abcam,Cat# ab32570; RRID: AB_777165 Goat Polyclonal

(9) Endothelial delivery of simvastatin by LRP1-targeted nanoparticles ameliorates pathogenesis of alzheimer''s disease in a mouse modelAlzheimer's research & therapyAugust 20, 2025Pan TT, Sun YY, Shi YF, Zhao M, Khan NU, Chen HY, Ji WL, Li J, Han L, Ma QH.(ab279297, Abcam), Rabbit-anti-GFAP (3670T, CST), Goat-anti-IBA-1 (AB5076, Abcam), Goat anti-PDGFRβ (AF385, R&D systems), Mouse anti-Aβ-6E10 (803015, biolegend), Rabbit anti-synaptophysin (SYN) (ab32127, Abcam),

(10) EBI2-oxysterol signalling regulates VE-cadherin expression and multiple sclerosis CD4+ T cell attachment to a human tri-cell spheroid blood-brain barrier modelBrain, Behavior, & Immunity - HealthAugust 1, 2025Fionä Caratis, Inez Mruk, Klaudia Konieczna-Wolska, Bartłomiej Rojek, Marek Hałas, Paulina Czaplewska, Bartosz Karaszewski, Tomomi Furihata, Aleksandra RutkowskaVCAM1 (AB_2809259, MA5-31965, ThermoFisher) (Wu et al., 2024), goat polyclonal PDGFRβ (RRID: AB_355339, AF385, R&D Systems) (Gao et al., 2024), rabbit monoclonal GFAP (RRID: AB_2631098, 12389, Cell Signalling)

(11) Integrating collecting systems in human kidney organoids through fusion of distal nephron to ureteric budCell stem cellJuly 3, 2025Shi M, Crouse B, Sundaram N, Pode Shakked N, Thorner K, King NM, Dutta P, Ester L, Zhang W, Govindarajah V, et al.RRID:AB_2154851 Goat anti-PAX2,R&D Systems,Cat# AF3364; RRID:AB_10889828 Goat anti-PDGFRB,R&D Systems,Cat# AF385; RRID:AB_355339 Rabbit anti-PECAM1,Cell Marque,Cat# 131R; RRID:AB_2893013 Goat anti-PODXL,R&D Systems,Cat#

(12) Generation and validation of a novel multitarget small molecule in glioblastomaCell Death DisApril 4, 2025Aizpea Artetxe-Zurutuza, Nerea Iturrioz-Rodriguez, Joseba Elizazu, Mireia Toledano-Pinedo, Alicia Porro-Pérez, Irati De Goñi, Alejandro Elua-Pinin, Linda Schäker-Hübner, Mikel Azkargorta, Felix Elortza, et al.with 4% PFA. Primary antibodies against phosphohistone H3 (pH3) (ab14955, abcam), Cleaved Caspase 3 (AF385, R&D Systems), Ki67 (ab15580, abcam) and PARP1 (ab32064, abcam) were used. Secondary antibodies were

(13) Development of Immunostaining Protocols for 3D Visualization of Pericytes in Human Retinal FlatmountsJournal of Histochemistry & CytochemistryApril 1, 2025Noëlle Bakker, Aïcha A. Croes, Eva Prevaes, Cornelis J. F. van Noorden, Reinier O. Schlingemann, Ingeborg KlaassenAb11575",1:1000,0.72 µg/ml NG2,Mouse,"Merck Life Science, MAB2029",1:100,10 µg/ml PDGFRβ,Goat,"R&D Systems, AF385",1:100,2 µg/ml αSMA,Mouse,"DAKO, M0851",1:250–1000,71–284 µg/l CD13,Mouse,"R&D Systems, MAB3815",1:200,2.5

(14) Intracerebral Hemorrhage-Associated Iron Release Leads to Pericyte-Dependent Cerebral Capillary Function DisruptionBiomoleculesJanuary 22, 2025Balk S, Panier F, Brandner S, Coras R, Blümcke I, Ekici AB, Sembill JA, Schwab S, Huttner HB, Sprügel MI.Triton X-100 (Sigma-Aldrich) in PBS. Slices were incubated with primary antibodies for PDGFRβ (R&D Systems, AF385, 1:200) and GPX-4 (Sigma-Aldrich, MABS1274, 4 µg/mL) overnight. After washing 3 times in PBS, secondary

(15) Plasma S100β is a predictor for pathology and cognitive decline in Alzheimer's diseaseFluids and barriers of the CNSJanuary 9, 2025Nehra G, Maloney BJ, Smith RR, Chumboatong W, Abner EL, Nelson PT, Bauer B, Hartz AMS.Information,Monoclonal,[EPR3915],Rabbit Platelet derived growth factor—beta subunit (PDGFRβ),Novus biologicals,AF385,B1W0621031,1:20,10 µg/mL,Mouse myeloma cell lineNS0-derived recombinant human PDGFRβ Leu33-Phe530 (Glu241Asp),Polyclonal,IgG,Goat Von

(16) Vascular Development of Fetal and Postnatal Neocortex of the Pig, the European Wild Boar Sus scrofaThe Journal of comparative neurologyDecember 1, 2024Sobierajski E, Czubay K, Beemelmans C, Beemelmans C, Meschkat M, Uhlenkamp D, Meyer G, Wahle P.(WB) Platelet‐derived growth factor receptor beta,"Goat, IF, WB","R&D Systems, Minneapolis, MN, USA, Cat# AF385, RRID: AB_355339",1:500 (IF) 1:1000 (WB) Platelet endothelial cell adhesion molecule 1,"Guinea pig,

(17) Perivascular NOTCH3+ Stem Cells Drive Meningioma Tumorigenesis and Resistance to RadiotherapyCancer DiscoveryOctober 4, 2024Abrar Choudhury, Martha A. Cady, Calixto-Hope G. Lucas, Hinda Najem, Joanna J. Phillips, Brisa Palikuqi, Naomi Zakimi, Tara Joseph, Janeth Ochoa Birrueta, William C. Chen, et al.(goat serum, BSA, albumin, glycine, and triton X in TBS) for 1h. Primary antibodies recognizing PDGFRB (AF385, R&D Systems, 1:200), CD34 (AF7227, R&D Systems, 1:200), or NOTCH3/N3ECD as described above were used

(18) Heterogeneous expression of the atypical chemokine receptor ACKR3 in glioblastoma patient-derived tissue samples and cell culturesScientific ReportsSeptember 20, 2024Damla Isci, Amandine Kuppens, Joshua Scalisi, Julie Cokaiko, Giulia D’Uonnolo, May Wantz, Martyna Szpakowska, Andy Chevigné, Bernard Rogister, Virginie Neirinckx#MAB42273, clone 11G8) was co-stained with SOX2 (Cell Signaling Technology, #3579), PDGFRβ (R&D systems, #AF385), Iba1 (Abcam, #ab5076), GFAP (Abcam, #ab4674), EGFR (Cell Signaling Technology, #4267), Rab5 (Cell Signaling Technology

(19) Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric budbioRxiv : the preprint server for biologySeptember 20, 2024Shi M, Crouse B, Sundaram N, Pode Shakked N, Ester L, Zhang W, Janakiram V, Kopan R, Helmrath MA, Bonventre JV, et al.

(20) Unveiling impaired vascular function and cellular heterogeneity in diabetic donor-derived vascular organoidsStem CellsSeptember 10, 2024Naderi-Meshkin H, Wahyu Setyaningsih WA, Yacoub A, Carney G, Cornelius VA, Nelson CA, Kelaini S, Donaghy C, Dunne PD, Amirkhah R, et al.at 4 °C with antibodies: ColIV (ab6311) for basement membrane, CD31 (Ab28364) for ECs and PDGFR-ß (AF385, R&D Systems) for pericytes. Samples were then washed 3 times with PBS and blocked in a 5% donkey serum

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PDGF R beta Antibody

PDGF R beta Antibody

PDGF R beta Antibody

PDGF R beta Antibody

PDGF R beta Antibody

Human PDGF Rß Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (92)

Reviews

Human PDGF Rß Antibody from R&D Systems, a Bio-Techne Brand

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