Human CD117/c-kit Antibody AF332 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman CD117/c-kit Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF332
IsotypeIgG
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Neutralization,Immunocytochemistry,CyTOF-ready
TargetCD117/c-kit
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse
Quantity100 ug (also 25 ug)
HostGoat
Conjugate/TagUnconjugated
NCBI Full Gene NameKIT proto-oncogene, receptor tyrosine kinase
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kit, Gln26-Thr520, Accession # P10721
ConcentrationLYOPH
PurityAntigen Affinity-purified
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
ClonalityPolyclonal
NCBI Gene AliasesMASTC, CD117, C-Kit, SCFR, PBT

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Decoding the heterogeneity of human undifferentiated spermatogonia reveals RAS-dependent regulation of stem cell fateCell ReportsFebruary 24, 2026Chiara Capponi, Christopher Smith, Alexa Medica, Tung-Chin Hsieh, Kyle E. Orwig, Kun TanMKI67,Novus Biologicals,Cat# NBP2-22112; RRID:AB_3266743 Goat polyclonal anti Human KIT,R&D Systems,Cat# AF332;RRID:AB_355302 Mouse monoclonal anti Human NANOS2,LifeSpan BioSciences,Cat# LS-C73159-100;RRID:AB_1599930 Mouse

(2) A spatiotemporal and machine-learning platform facilitates the manufacturing of hPSC-derived esophageal mucosaDevelopmental CellMay 1, 2025Ying Yang, Carmel Grace McCullough, Lucas Seninge, Lihao Guo, Woo-Joo Kwon, Yongchun Zhang, Nancy Yanzhe Li, Sadhana Gaddam, Cory Pan, Hanson Zhen, et al.anti-EPCAM-BX091,Akoya Biosciences,Cat# 4550088; RRID: AB_2935888 Goat polyclonal anti-cKIT,R&D systems,Cat# AF332; RRID: AB_355302 Rabbit monoclonal anti-FBN,Abcam,Cat# ab32419; RRID: AB_732379 Goat polyclonal anti-DCN,R&D systems

(3) TMEM16A in smooth muscle cells acts as a pacemaker channel in the internal anal sphincterCommunications BiologyFebruary 5, 2024Ping Lu, Lawrence M. Lifshitz, Karl Bellve, Ronghua ZhuGepolyclonal antibody against TMEM16A (ab53212, 1:100; Abcam) and a goat polyclonal antibody against c-Kit (AF332, 1:20; R&D Systems) for TMEM16AICCKO mouse samples and their isogenic controls. Alternatively, we used

(4) Activation of the receptor KIT induces the secretion of exosome-like small extracellular vesiclesJ Extracell BiolJanuary 1, 2024Pfeiffer A, Bandara G, Petersen JD, Falduto GH, Zimmerberg J, Metcalfe DD, Olivera A.primary antibodies were used at a dilution of 1:1000 unless stated otherwise: goat anti‐KIT (R&D Systems, AF332), rabbit anti‐pKIT Tyr823 (Cell Signaling Technologies, 77522), mouse anti‐JAK2 (Invitrogen, MA5‐15632),

(5) In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypicSTAR protocolsMay 25, 2023Phua R, Tien TZ, Goh G, Pantelireis N, Sim KSH, Lim J, Clavel C.1:100,"Santa Cruz Biotechnology, USA",sc-166857 Human CD117/c-kit AntibodyDilution: 1:100,"R&D Systems, USA",AF332 Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)Dilution: 1:1000,"Abcam, UK",ab150073 Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594

(6) FOXP4 promotes proliferation of human spermatogonial stem cellsAsian journal of andrologyMay 1, 2023Luo SW, Tang L, Zhou D, Bo H, Fan LQ.4°C GFRα1,R&Dcat#AF560,1:25,16 h at 4°C PCNA,Abcam cat#ab29,1:50,12–16 h at 4°C KIT,R and D cat#AF332,1:25,16 h at 4°C Secondary antibody,,, "Donkey anti-Rabbit IgG, Alexa Fluor 488",Thermo Fisher Scientific

(7) Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle- and exosome-like populations of KIT-containing extracellular vesiclesJournal of extracellular vesiclesSeptember 1, 2022Pfeiffer A, Petersen JD, Falduto GH, Anderson DE, Zimmerberg J, Metcalfe DD, Olivera A.tilting overnight at 4°C. For the immunocapture of KIT‐containing EVs, 3 μg of KIT antibody (R&D Systems, AF332, directed against the N‐terminal domain of KIT [Gln26‐Thr520], goat; Santa Cruz, sc‐13508, directed

(8) Oscillating calcium signals in smooth muscle cells underlie the persistent basal tone of internal anal sphincterJournal of cellular physiologyAugust 1, 2021Lu P, Chen J, Zhang C, Saur D, Baer CE, Lifshitz LM, Fogarty KE, ZhuGe R.polyclonal antibody to TMEM16A (ab53212, 1:100; Abcam) and a goat polyclonal antibody against c-Kit (AF332, 1:20; R&D systems). The specificity of these antibodies has been established by vendors and others.

(9) CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathwayCell discoveryNovember 17, 2020Cui W, He X, Zhai X, Zhang H, Zhang Y, Jin F, Song X, Wu D, Shi Q, Li L.

(10) The dynamic transcriptional cell atlas of testis development during human pubertyYearbook of Paediatric EndocrinologyOctober 23, 2020Guo J et al.1:250 Abcam cat# ab133273, RRID:AB_11156085 Goat polyclonal anti-KIT Dilution: 1:25 R&D systems cat# AF332, RRID:AB_355302 Rabbit polyclonal anti-INHA Dilution: 1:1000 Atlas Antibodies cat# HPA019141, RRID:AB_1851789 Mouse

(11) Gender-Related Differences of Tachykinin NK₂ Receptor Expression and Activity in Human Colonic Smooth MuscleThe Journal of pharmacology and experimental therapeuticsOctober 1, 2020Drimousis S, Markus I, Murphy TV, Perera DS, Phan-Thien KC, Zhang L, Liu L.actin (a-SMA) antibody (ab7817, 1:50; Abcam, Melbourne, VIC, Australia), the anti-cD117/c-kit antibody (AF332, 1:50; R&D Systems, Minneapolis, MN), the anti–HuC/ D antibody (A21271, 1:200; Invitrogen, Carlsbad,

(12) Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian SpermatogenesisDevelopmental cellAugust 24, 2020Shami AN, Zheng X, Munyoki SK, Ma Q, Manske GL, Green CD, Sukhwani M, Orwig KE, Li JZ, Hammoud SS.Antibody,Company,Product #,Dilution UCHL1(PGP9.5),BioRad,7863–1004,1:100 cKIT(CD117),R&D systems,AF332,1:50 TCF3,Sigma-Aldrich,HPA062476,1:50 GPX1,Sigma-Aldrich,HPA044758,1:500 FMR1,Sigma-Aldrich,HPA050118,1:100 MORC1,Thermo Fisher

(13) The Dynamic Transcriptional Cell Atlas of Testis Development during Human PubertyCell stem cellFebruary 6, 2020Guo J, Nie X, Giebler M, Mlcochova H, Wang Y, Grow EJ, DonorConnect., Kim R, Tharmalingam M, Matilionyte G, et al.1:250,Abcam,"cat# ab133273, RRID:AB_11156085" Goat polyclonal anti-KIT Dilution: 1:25,R&D systems,"cat# AF332, RRID:AB_355302" Rabbit polyclonal anti-INHA Dilution: 1:1000,Atlas Antibodies,"cat# HPA019141, RRID:AB_1851789" Mouse

(14) PLZF^posc-KIT^pos-delineated A₁-A₄-differentiating spermatogonia by subset and stage detection upon Bouin fixationAsian journal of andrologyMay 1, 2019Tang RL, Fan LQ.dilution; SC-22839, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-c-KIT (1:50 dilution; AF332, R&D Systems, Minneapolis, MN, USA), and goat anti-GFRA1 (1:50 Asian Journal of Andrology dilution;

(15) The effects of restricted glycolysis on stem-cell like characteristics of breast cancer cellsOncotargetMay 1, 2018Banerjee A, Arvinrad P, Darley M, Laversin SA, Parker R, Rose-Zerilli MJJ, Townsend PA, Cutress RI, Beers SA, Houghton FD, et al.to assay. Paclitaxel and Imatinib mesylate were from Sigma Aldrich, UK. Human CD117/c-KIT antibody (AF332) and IgG control from R&D Systems, UK. All photo-microscopy of cells was performed using an Olympus

(16) Chromatin and Single-Cell RNA-Seq Profiling Reveal Dynamic Signaling and Metabolic Transitions during Human Spermatogonial Stem Cell DevelopmentCell Stem CellOctober 5, 2017Jingtao Guo, Edward J. Grow, Chongil Yi, Hana Mlcochova, Geoffrey J. Maher, Cecilia Lindskog, Patrick J. Murphy, Candice L. Wike, Douglas T. Carrell, Anne Goriely, James M. Hotaling, Bradley R. CairnsMC813, RRID: AB_1264259" Goat polyclonal anti-c-KIT (CD117); Dilution: 1:25 - 1:100,R&D Systems,"cat# AF332, RRID: AB_355302" Rabbit polyclonal anti-cFGFR3; dilution: 1:500,Santa Cruz Biotechnology,"cat# sc390423,

(17) Use of Patterned Collagen Coated Slides to Study Normal and Scleroderma Lung Fibroblast MigrationScientific reportsJune 1, 2017Ahmed Abdi B, Lopez H, Karrar S, Renzoni E, Wells A, Tam A, Etomi O, Hsuan JJ, Martin GR, Shiwen X, et al.typically 0.06–0.36 μg/mL in the presence of 20 ng/ml Recombinant Human SCF/c‑kit Ligand, R&D Systems #AF332) (C).

(18) Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cellsFertility and sterilityAugust 1, 2014Valli H, Sukhwani M, Dovey SL, Peters KA, Donohue J, Castro CA, Chu T, Marshall GR, Orwig KE.anti-ZBTB16 (1:50; AF2944, R&D Systems), rabbit anti- KIT; goat anti-KIT (1:400; A4502, DakoCytomation; 1:50; AF332, R&D Systems), rabbit anti-SALL4 (1:500; ab29112, Abcam; 1:40; ab181087, Abcam), mouse anti-ENO2 (1:500,

(19) N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiationBiochemical and biophysical research communicationsSeptember 6, 2013Ishimine H, Yamakawa N, Sasao M, Tadokoro M, Kami D, Komazaki S, Tokuhara M, Takada H, Ito Y, Kuno S, et al.incubated with antibodies against N-cadherin (C3865, Sigma, 1:200), Flk1 (10347, IBL, 1:100), c-kit (AF332, R&D Systems, 1:200), Integrin-α4 (sc-14008, R&D Systems, 1:200), vascular cell adhesion molecule 1 (VCAM-1;

(20) Motesanib inhibits Kit mutations associated with gastrointestinal stromal tumorsJournal of experimental & clinical cancer research : CRJuly 15, 2010Caenepeel S, Renshaw-Gegg L, Baher A, Bush TL, Baron W, Juan T, Manoukian R, Tasker AS, Polverino A, Hughes PE.added to a 96-well DELFIA microplate (PerkinElmer Inc.) coated with anti-Kit antibody (1 μg per well; AF332, R&D Systems, Inc.; Minneapolis, MN) and incubated for 2 hours. Lysates were then removed and the plate

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CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

Human CD117/c-kit Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (23)

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Human CD117/c-kit Antibody from R&D Systems, a Bio-Techne Brand

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