Human TNF RI/TNFRSF1A Antibody AF225 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman TNF RI/TNFRSF1A Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF225
NCBI Gene AliasesTNF-R, p60, FPF, TNFAR, TNFR60, CD120a, TNF-R-I, TNFR55, TBP1, TNFR1, p55, TNF-R55, p55-R
ApplicationsELISA Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Agonist Activity,Neutralization,Immunocytochemistry,CyTOF-ready,Functional Assay
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
IsotypeIgG
HostGoat
TargetTNF RI/TNFRSF1A
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Porcine
NCBI Full Gene NameTNF receptor superfamily member 1A
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human sTNF RI/TNFRSF1A, Accession # NP_001056
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Opposing regulation of the K63-linked polyubiquitination of RIPK3 by SMURF1 and USP5 in necroptosisNature communicationsAugust 9, 2025Hwang CH, Lee M, Kim JW, Nam YW, Hwang G, Ryu HS, Seo J, Lee EW, Ko HW, Song J.Cell Signaling), IκBα (#4814, Cell Signaling), phospho-IκBα (Ser539) (#2859, Cell Signaling), TNFR1 (#AF225;R&D Systems), TRADD (#610572; BD transduction), Ubiqutin (P4D1) mouse mAB(HRP conjugate) (#14049, Cell Signaling

(2) EGFR inhibits TNF-α-mediated pathway by phosphorylating TNFR1 at tyrosine 360 and 401Cell death and differentiationOctober 1, 2024Nam YW, Shin JH, Kim S, Hwang CH, Lee CS, Hwang G, Kim HR, Roe JS, Song J.

(3) APOL1 promotes endothelial cell activation beyond the glomerulusiScienceJune 16, 2023Carracedo M, Ericson E, Ågren R, Forslöw A, Madeyski-Bengtson K, Svensson A, Riddle R, Christoffersson J, González-King Garibotti H, Lazovic B, et al.primary antibodies overnight detecting CD31 (Abcam, ab24590, clone P2B1) or TNFRSF1A (R&D systems, AF225). After washing with PBS (Gibco, 14190094), cells were incubated with secondary antibodies (STAR Methods

(4) Structural insights into the disruption of TNF-TNFR1 signalling by small molecules stabilising a distorted TNFNature communicationsJanuary 25, 2021McMillan D, Martinez-Fleites C, Porter J, Fox D 3rd, Davis R, Mori P, Ceska T, Carrington B, Lawson A, Bourne T, et al.

(5) CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic MalignanciesMolecular therapy : the journal of the American Society of Gene TherapyFebruary 5, 2020Rodriguez-Garcia A, Sharma P, Poussin M, Boesteanu AC, Minutolo NG, Gitto SB, Omran DK, Robinson MK, Adams GP, Simpkins F, et al.

(6) Small molecules that inhibit TNF signalling by stabilising an asymmetric form of the trimerNature communicationsDecember 19, 2019O'Connell J, Porter J, Kroeplien B, Norman T, Rapecki S, Davis R, McMillan D, Arakaki T, Burgin A, Fox Iii D, et al.

(7) Aspirin Action in Endothelial Cells: Different Patterns of Response Between Chemokine CX3CL1/CX3CR1 and TNF-α/TNFR1 Signaling PathwaysCardiovascular drugs and therapyJune 1, 2015Szukiewicz D, Wojciechowska M, Bilska A, Stangret A, Szewczyk G, Mittal TK, Watroba M, Kochanowski J.immunohistochemical staining procedure was utilized with a goat anti-human polyclonal antibody IgG to TNF R1/TNFRSF1A (AF225; R&D Systems, Inc., USA; concentration of 10 μg/ml) as the primary antibody, and an Anti-Goat HRP-AEC Cell & Tissue Staining Kit

(8) Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signalingThe Journal of biological chemistryApril 17, 2015Wang YB, Tan B, Mu R, Chang Y, Wu M, Tu HQ, Zhang YC, Guo SS, Qin XH, Li T, et al.

(9) NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based VaccinePloS oneJanuary 1, 2015Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud VM, Anjuère F.

(10) EI24 regulates epithelial-to-mesenchymal transition and tumor progression by suppressing TRAF2-mediated NF-κB activityOncotargetDecember 1, 2013Choi JM, Devkota S, Sung YH, Lee HW.anti2392 Oncotarget 2013; 4: TRADD (Santa Cruz Biotechnology, #sc-7868), antiTNFR1 (R&D systems #AF225 for IP; Santa Cruz Biotechnology #sc-8436 for IB), anti-poly (ADP-ribose) polymerase (PARP; Cell Signaling Technology

(11) Pellino3 targets RIP1 and regulates the pro-apoptotic effects of TNF-αNature communicationsJanuary 1, 2013Yang S, Wang B, Tang LS, Siednienko J, Callanan JJ, Moynagh PN.(1:1,000) and anti-FADD (1F7; AAM212) (1:1,000) were from Enzo Life Science; anti-human and mouse TNFR1 (AF225; AF-425-PB) (for immunoprecipitations) (1:1,000) and anti-human and mouse Pan-cIAP (MAB3400) (1:1,000)

(12) Involvement of the same TNFR1 residue in mendelian and multifactorial inflammatory disordersPloS oneJanuary 1, 2013Jéru I, Charmion S, Cochet E, Copin B, Duquesnoy P, Garcia MT, Le Borgne G, Cathebras P, Gaillat J, Karabina S, et al.transferred onto PVDF membranes. Western blotting was performed with a goat polyclonal anti-TNFR1 antibody (AF225, R&D Systems), a mouse monoclonal anti-a tubulin antibody (Santa-Cruz Biotechnology), as well as HRP-conjugated

(13) Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritisJournal of immunological methodsApril 30, 2012Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK.

(14) Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayersDigestive diseases and sciencesOctober 1, 2007van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH.

(15) Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expressionAtherosclerosisMay 1, 2007Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF.

(16) Tumour necrosis factor-alpha in Barrett's oesophagus: a potential novel mechanism of actionOncogeneSeptember 5, 2002Tselepis C, Perry I, Dawson C, Hardy R, Darnton SJ, McConkey C, Stuart RC, Wright N, Harrison R, Jankowski JA.(Amersham-Pharmacia), blocked with 10% skimmed milk and incubated in either a goat polyclonal TNFR1 antibody (1 mg/ml; AF225, R&D Systems) or a rabbit polyclonal TNF-a antibody (5 mg/ml; CY 007, Immunogenetics). After extensive

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TNF RI/TNFRSF1A Antibody

Human TNF RI/TNFRSF1A Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (16)

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Human TNF RI/TNFRSF1A Antibody from R&D Systems, a Bio-Techne Brand

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