Mouse TNF RI/TNFRSF1A Antibody AF-425-PB from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse TNF RI/TNFRSF1A Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-425-PB
NCBI Gene AliasesTNF-R, TNFalpha-R1, FPF, TNFAR, TNFR60, CD120a, TNF-R-I, TNFRI, Tnfr1, Tnfr-2, TNFRp55, TNF-R1, p55, TNF-alphaR1, TNF-R55, p55-R
ApplicationsComplex Application,ELISA Development,IP Western,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Agonist Activity,Immunocytochemistry,CyTOF-ready
Conjugate/TagUnconjugated
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetTNF RI/TNFRSF1A
ReactivityHuman,Mouse,Rat,Transgenic Mouse
HostGoat
Quantity100 ug (also 25 ug)
NCBI Full Gene Nametumor necrosis factor receptor superfamily, member 1a
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse TNF RI, Ile22-Ala212, Accession # P25118
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study TNFR1 in mouse models, and if so, how has it been utilized in research?

Yes, the TNF RI/TNFRSF1A antibody has been shown to work effectively in mouse models. For instance, in a study investigating TRAPS mutations, the antibody was used to analyze TNFR1 expression in bone marrow-derived macrophages, demonstrating its utility in examining inflammatory responses in vivo and in vitro ().

What applications is this antibody suitable for, and are there any specific findings from studies that utilized it?

The TNF RI/TNFRSF1A antibody is suitable for applications including Western blotting, ELISA, immunocytochemistry, and flow cytometry. In one study, it was employed to assess TNFR1 levels in various cell types, revealing that TRAPS mutations led to decreased cell surface expression of TNFR1, which affected inflammatory responses ().

How does this antibody perform in distinguishing between mouse and human TNFR1?

The antibody has been shown to detect mouse TNF RI/TNFRSF1A with less than 5% cross-reactivity with recombinant human TNF RI in direct ELISAs and Western blots, making it a reliable tool for studies focused on mouse models while minimizing interference from human proteins.

Are there any notable studies that have cited this antibody in the context of TNF signaling pathways?

Yes, the TNF RI/TNFRSF1A antibody has been cited in multiple studies exploring TNF signaling pathways. For example, it was used to investigate the role of RIPK1 in TNF receptor signaling, highlighting its importance in mediating cell death and inflammatory responses in various experimental setups (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) PARP12-mediated mono-ADP-ribosylation as a checkpoint for necroptosis and apoptosisProceedings of the National Academy of SciencesJune 17, 2025Xin Huang, Fangxia Li, Lin Liu, Yanxia Li, Mengmeng Zhang, Guoming Ma, Ying Gao, Bing Shan, Xiaozhen Liang, Junying Yuan, et al.(CST,8592); Caspase-8 (CST,4927); STAT1 (CST, 9172); Phospho-Tyr701-Stat1 (CST, 7649); TNFR1 (R&D Systems AF-425-PB); ZBP1 (AdipoGen, AG-20B-0010); Caspase-1 (p20) (AdipoGen, AG-20B-0042-C100); β-actin (TransGen, I10813).

(2) Myocardial infarction augments sleep to limit cardiac inflammation and damageNatureNovember 1, 2024Huynh P, Hoffmann JD, Gerhardt T, Kiss MG, Zuraikat FM, Cohen O, Wolfram C, Yates AG, Leunig A, Heiser M, et al.primary antibodies overnight at 4 °C: anti-CCR2 (BD Bioscience, 747968), anti-TNFRSF1A (R&D Systems, AF-425-PB), anti-GFP (Biolegend, 338001), anti-collagen 3 (Southern Biotech, 1330–01), anti-collagen 4 (Southern Biotech

(3) In vivo single-cell CRISPR uncovers distinct TNF programmes in tumour evolutionNatureAugust 1, 2024Renz PF, Ghoshdastider U, Baghai Sain S, Valdivia-Francia F, Khandekar A, Ormiston M, Bernasconi M, Duré C, Kretz JA, Lee M, et al.

(4) TNFR1 mediates heterogeneity in single-cell NF-κB activationiScienceApril 19, 2024Chieh-Teng Cheng, Jye-Chian Hsiao, Alexander Hoffmann, Hsiung-Lin Tupolyvinylidene difluoride membranes using the Bio-Rad system. Antibodies used in this study include: Anti-TNFR1 (AF-425-PB, R&D Systems), anti-GADPH (MAB374, Merck), donkey anti-goat IgG-HRP (sc-2020, Santa Cruz Biotechnology),

(5) Small molecule activators of TAK1 promotes its activity-dependent ubiquitination and TRAIL-mediated tumor cell deathProceedings of the National Academy of SciencesSeptember 26, 2023Weimin Sun, Guowei Wu, Xinyu Tian, Chunting Qi, Jingli Liu, Yilun Tong, Mengmeng Zhang, Jiayang Gao, Ze Cao, Yuchao Zhang, et al.p-TAK1 (Cell Signaling Technology, #4536); Syk (Cell Signaling Technology, #13198); TNFR1 (R&D Systems, AF-425-PB); Recombinant hTNFα (Novoprotein, #C008); Flag antibody-conjugated agarose (Sigma, #A2220); Ni-NTA His-Bind Resin

(6) CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathologyNature immunologyNovember 1, 2022Knizkova D, Pribikova M, Draberova H, Semberova T, Trivic T, Synackova A, Ujevic A, Stefanovic J, Drobek A, Huranova M, et al.from Santa Cruz Biotechnology; murine IL-17RA (MAB4481), IL-17RC (AF2270), IL-17RD (AF2276) and TNFR1 (AF-425-PB) were from R&D Systems; and TRAF6 (ab40675) was from Abcam. The standard dilution of antibodies for immunoblotting

(7) PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIP(L) to promote TNFα-mediated cell deathCell death & diseaseSeptember 7, 2022Wu G, Li D, Liang W, Sun W, Xie X, Tong Y, Shan B, Zhang M, Lu X, Yuan J, et al.Cell Signaling Technology. MLKL (ab183770), p-S345-MLKL (ab196436), and FADD (ab124812) were purchased from Abcam. TNFR1 (AF-425-PB) and pan-cIAP (MAB3400) were purchased from R&D Systems. β-actin (I10813) was purchased from TransGen.

(8) ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-κBCell death and differentiationJune 1, 2022Won M, Park KA, Kim S, Ju E, Ko Y, Yoo H, Ro H, Lee J, Oh J, Lee EG, et al.

(9) TAB2 deficiency induces dilated cardiomyopathy by promoting RIPK1-dependent apoptosis and necroptosisThe Journal of clinical investigationFebruary 15, 2022Yin H, Guo X, Chen Y, Zeng Y, Mo X, Hong S, He H, Li J, Steinmetz R, Liu Q.from MilliporeSigma; anti–TNF-α (catalog AF7014) was from Affinity Biosciences; anti-TNFR1 (catalog AF-425-PB) was from R&D Systems; and anti-ANP (catalog PA5-29559) was from Thermo Fisher Scientific. Mouse models. Tab2fl/fl

(10) TRAPS mutations in Tnfrsf1a decrease the responsiveness to TNFα via reduced cell surface expression of TNFR1Frontiers in immunologyJanuary 1, 2022Akagi T, Hiramatsu-Asano S, Ikeda K, Hirano H, Tsuji S, Yahagi A, Iseki M, Matsuyama M, Mak TW, Nakano K, et al.Ser536 (3033), ATF-6 (65880), IRE1α (3294; all from Cell Signaling Technology, Danvers, MA, USA), TNFR1 (AF-425-PB, R&D Systems, Minneapolis, MN, USA), TNFR2 (ab109322, Abcam, Cambridge, UK), and actin (A2066, Sigma-Aldrich).

(11) A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domainNature cell biologyJune 1, 2019Polykratis A, Martens A, Eren RO, Shirasaki Y, Yamagishi M, Yamaguchi Y, Uemura S, Miura M, Holzmann B, Kollias G, et al.

(12) Chaperone-mediated autophagy is involved in the execution of ferroptosisProceedings of the National Academy of Sciences of the United States of AmericaFebruary 19, 2019Wu Z, Geng Y, Lu X, Shi Y, Wu G, Zhang M, Shan B, Pan H, Yuan J.p-S166– RIPK1 (Biolynx); p-S345–MLKL (ab196436; Abcam); p-S358–MLKL (91689; Cell Signaling Technology); TNFR1 (AF-425-PB; R&D Systems); Myc (C3956; Sigma–Aldrich); GPX4 (ab125066; Abcam); HSP90-α (13171-1-AP; Proteintech); HSP90-β

(13) Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosisProceedings of the National Academy of Sciences of the United States of AmericaFebruary 27, 2018Meng H, Liu Z, Li X, Wang H, Jin T, Wu G, Shan B, Christofferson DE, Qi C, Yu Q, et al.(Cell Signaling Technology 3493); p-S166 RIPK1 (Cell Signaling Technology 31122); TNFR1 (R&D Systems AF-425-PB); Myc (Sigma Aldrich C3956); GFP (Santa Cruz sc-9996); FADD [Abcam ab124812 and Santa Cruz (6036)];

(14) ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSCNature cell biologyJanuary 1, 2018Dziedzic SA, Su Z, Jean Barrett V, Najafov A, Mookhtiar AK, Amin P, Pan H, Sun L, Zhu H, Ma A, et al.1:300 Santa Cruz, Cat# sc-374639), TNFR1 (1:1,000 Cell Signaling, Cat# 13377S and R&D Systems, Cat# AF-425-PB), A20 (1:1,000 Cell Signaling, Cat# 5630), IKKβ (1:1,000 BD Technologies, Cat# 611254), cIAP1 (1:5 custom

(15) Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injuryCell death & diseaseJune 29, 2017Martens S, Jeong M, Tonnus W, Feldmann F, Hofmans S, Goossens V, Takahashi N, Bräsen JH, Lee EW, Van der Veken P, et al.or HT-29 cells. After the lysis step, each complex was purified by IP using 1 μg of α-mTNFR1 (R&D systems, AF-425-PB) for TNFRI complex I, 1 μg of α-mFADD (Santa cruz, sc-6036) for mouse necrosome or 1 μg of α-hRIPK3

(16) SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitinationGenes & developmentJune 1, 2017Wei R, Xu LW, Liu J, Li Y, Zhang P, Shan B, Lu X, Qian L, Wu Z, Dong K, et al.from Dr. William Hahn. The following commercial antibodies and reagents were used: TNFR1 (R&D Systems, AF-425-PB; and Abcam, ab19139), RIPK1 (Cell Signaling Technology, 3493), TRADD (Santa Cruz Biotechnology, sc-7868),

(17) Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammationScientific reportsMarch 13, 2017Arokiasamy S, Zakian C, Dilliway J, Wang W, Nourshargh S, Voisin MB.

(18) Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesiclesEMBO molecular medicineOctober 1, 2016Balusu S, Van Wonterghem E, De Rycke R, Raemdonck K, Stremersch S, Gevaert K, Brkic M, Demeestere D, Vanhooren V, Hendrix A, et al.

(19) Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progressionNature medicineNovember 1, 2015Alam MS, Gaida MM, Bergmann F, Lasitschka F, Giese T, Giese NA, Hackert T, Hinz U, Hussain SP, Kozlov SV, et al.(RB6-8C5; eBiosciences), MHC class II (M5 1114.15.2 ; eBiosciences), TNF-α (MP6-XT22; eBiosciences), TNFR1 (AF-425-PB; R&D systems), and IFNγ (XMG1.2; eBiosciences). GST was detected with B-14 (Santa Cruz Biotechnology).

(20) Tumor necrosis factor (TNF)-receptor 1 and 2 mediate homeostatic synaptic plasticity of denervated mouse dentate granule cellsScientific reportsAugust 6, 2015Becker D, Deller T, Vlachos A.were treated with activating antibodies for TNFR1 or TNFR2 (TNFR1-AB and TNFR2-AB, 2 μg/ml; AF-426-PB, AF-425-PB, R&D systems, USA) for 3d. Laser capture microdissection (LMD) of re-sliced cultures Slice cultures

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TNF RI/TNFRSF1A Antibody

TNF RI/TNFRSF1A Antibody

TNF RI/TNFRSF1A Antibody

TNF RI/TNFRSF1A Antibody

TNF RI/TNFRSF1A Antibody

Mouse TNF RI/TNFRSF1A Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (33)

Reviews

Mouse TNF RI/TNFRSF1A Antibody from R&D Systems, a Bio-Techne Brand

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