IGF-II/IGF2 Antibody AF-292-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIGF-II/IGF2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF-292-NA
Quantity250 ug (also 25 ug)
ApplicationsAffinity Assay,ELISA Microarray Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Neutralization,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ConcentrationLYOPH
PurityAntigen Affinity-purified
IsotypeIgG
HostGoat
TargetIGF-II/IGF2
ReactivityHuman,Mouse,Porcine,Transgenic Mouse
Antibody TypePrimary Antibodies
NCBI Gene AliasesSRS3, GRDF, C11orf43, PP9974, IGF-II
NCBI Full Gene Nameinsulin like growth factor 2
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human IGF-II/IGF2, Ala25-Glu91, Accession # P01344
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human IGF-II/IGF2 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IGF-II/IGF2 is observed and less than 1% cross-reactivity with recombinant human IGF-I/IGF1 is observed.

Sizes Available: 250 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Reprogramming of fibroblasts into cancer-associated fibroblasts via IGF2-mediated autophagy promotes metastasis of lung cancer cellsiScienceDecember 20, 2024Limin Cao, Bingbing Li, Sijia Zheng, Qicheng Zhang, Yongmei Qian, Yinghui Ren, Huimin Wang, Min Wang, Xiang Wu, Jiayi Zhang, et al.goat anti-mouse IgG,ZSGB-BIO,Cat#ZB2305; RRID:AB_2747415 IGF2 neutralizing antibody,R&D Systems,Cat#AF-292-NA; RRID:AB_354449 CXCL12 neutralizing antibody,R&D Systems,Cat#MAB310; RRID:AB_2276927 ,, "Chemicals,

(2) Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expressionNature communicationsJune 8, 2024Jang HJ, Min HY, Kang YP, Boo HJ, Kim J, Ahn JH, Oh SH, Jung JH, Park CS, Park JS, et al.

(3) Novel Isoform DTX3c Associates with UBE2N-UBA1 and Cdc48/p97 as Part of the EphB4 Degradation Complex Regulated by the Autocrine IGF-II/IR(A) Signal in Malignant MesotheliomaInternational journal of molecular sciencesApril 17, 2023Scalia P, Merali C, Barrero C, Suma A, Carnevale V, Merali S, Williams SJ.

(4) PRC2 Heterogeneity Drives Tumor Growth in MedulloblastomaCancer researchAugust 16, 2022Yi J, Kim B, Shi X, Zhan X, Lu QR, Xuan Z, Wu J.media were changed to serum free media or serum free media with 2 ug/ml IGF2 antibody (R&D Systems Cat# AF-292-NA, RRID:AB_354449) for another 72 hours, or 200 ng/ml recombinant human IGF2 (#292-G2, R&D Systems) for

(5) The imprinted Igf2-Igf2r axis is critical for matching placental microvasculature expansion to fetal growthDevelopmental cellJanuary 10, 2022Sandovici I, Georgopoulou A, Pérez-García V, Hufnagel A, López-Tello J, Lam BYH, Schiefer SN, Gaudreau C, Santos F, Hoelle K, et al.the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1, 1:50,000, R&D Systems – AF3787). After 2 × 10 min washes with milliQ water

(6) Insulin-like growth factor II prevents oxidative and neuronal damage in cellular and mice models of Parkinson's diseaseRedox biologyOctober 1, 2021Martín-Montañez E, Valverde N, Ladrón de Guevara-Miranda D, Lara E, Romero-Zerbo YS, Millon C, Boraldi F, Ávila-Gámiz F, Pérez-Cano AM, Garrido-Gil P, et al.by its own receptor, as there are no specific antagonists, the antibody AF-292 (20 ng/mL) (Cat nº: AF-292-NA, R&D System) against the IGF-IIr was used [13,27,28]. To check the involvement of IGF-IIr on the main

(7) Insulin-like growth factor II prevents oxidative and neuronal damage in cellular and mice models of Parkinson's diseaseRedox biologyOctober 1, 2021Martín-Montañez E, Valverde N, Ladrón de Guevara-Miranda D, Lara E, Romero-Zerbo YS, Millon C, Boraldi F, Ávila-Gámiz F, Pérez-Cano AM, Garrido-Gil P, et al.by its own receptor, as there are no specific antagonists, the antibody AF-292 (20 ng/mL) (Cat nº: AF-292-NA, R&D System) against the IGF-IIr was used [13,27,28]. To check the involvement of IGF-IIr on the main

(8) Integrated Genomic Analysis Identifies Driver Genes and Cisplatin-Resistant Progenitor Phenotype in Pediatric Liver CancerCancer discoveryOctober 1, 2021Hirsch TZ, Pilet J, Morcrette G, Roehrig A, Monteiro BJE, Molina L, Bayard Q, Trépo E, Meunier L, Caruso S, et al.tested (10μM). Each concentration was tested in duplicate. Polyclonal goat anti-IGF2 antibody (Ref AF-292-NA, Biotechne) and control anti-Igg goat antibody (Ref AB-108-C, Bio-techne) were tested using 5 concentrations

(9) Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and functionPLoS geneticsOctober 1, 2020Hammerle CM, Sandovici I, Brierley GV, Smith NM, Zimmer WE, Zvetkova I, Prosser HM, Sekita Y, Lam BYH, Ma M, et al.

(10) IGF and mTOR pathway expression and in vitro effects of linsitinib and mTOR inhibitors in adrenocortical cancerEndocrineJune 1, 2019De Martino MC, van Koetsveld PM, Feelders RA, de Herder WW, Dogan F, Janssen JAMJL, Hofste Op Bruinink D, Pivonello C, Waaijers AM, Colao A, et al.1:500) and the primary polyclonal antibodies to detect IGF2 and IGF2R were purchased from R&D Systems (AF-292-NA; dilution: 1:500) and Santa Cruz Biotech (SC-25462; dilution: 1:50) respectively. The slides were washed

(11) Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJanuary 30, 2018Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ.

(12) A journey through growth plates: tracking differences in morphology and regulation between the spine and the long bones in a pig modelThe spine journal : official journal of the North American Spine SocietyNovember 1, 2017Raimann A, Javanmardi A, Egerbacher M, Haeusler G.

(13) Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progressionNature communicationsFebruary 10, 2017Xu WW, Li B, Guan XY, Chung SK, Wang Y, Yip YL, Law SY, Chan KT, Lee NP, Chan KW, et al.

(14) Conversion to stem-cell state in response to microenvironmental cues is regulated by balance between epithelial and mesenchymal features in lung cancer cellsMolecular oncologyFebruary 1, 2016Andriani F, Bertolini G, Facchinetti F, Baldoli E, Moro M, Casalini P, Caserini R, Milione M, Leone G, Pelosi G, et al.Technologies) was used at 1 ng/ml. TGFβ1 and IGFII neutralizing antibodies (clone #9016 and catalog #AF‐292‐NA respectively, R&D Systems) were used at 0.5 μg/ml. To avoid culture conditions artifacts that could

(15) A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell lineInternational journal of oncologyOctober 1, 2014Benabbou N, Mirshahi P, Bordu C, Faussat AM, Tang R, Therwath A, Soria J, Marie JP, Mirshahi M.

(16) 'Big'-insulin-like growth factor-II signaling is an autocrine survival pathway in gastrointestinal stromal tumorsThe American journal of pathologyJuly 1, 2012Rikhof B, van der Graaf WT, Suurmeijer AJ, van Doorn J, Meersma GJ, Groenen PJ, Schuuring EM, Meijer C, de Jong S.

(17) Feeder-free maintenance of hESCs in mesenchymal stem cell-conditioned media: distinct requirements for TGF-beta and IGF-IICell ResearchJune 1, 2009Rosa Montes et al.specific ligand neutralization through addition of 2 μg/ml of the antibody α-hIGF-II (R&D Systems; AF-292-NA), as described previously [5]. As an appropriate control, an immunoglobulin isotype match was used.

(18) Insulin-like growth factor-II is increased in systemic sclerosis-associated pulmonary fibrosis and contributes to the fibrotic process via Jun N-terminal kinase- and phosphatidylinositol-3 kinase-dependent pathwaysThe American journal of pathologyJune 1, 2008Hsu E, Feghali-Bostwick CA.

(19) Development and validation of sandwich ELISA microarrays with minimal assay interferenceJournal of proteome researchJune 1, 2008Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC.

(20) The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pyloriGastroenterologyMay 1, 2006McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, et al.

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IGF-II/IGF2 Antibody

IGF-II/IGF2 Antibody

IGF-II/IGF2 Antibody

IGF-II/IGF2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (22)

Reviews

IGF-II/IGF2 Antibody from R&D Systems, a Bio-Techne Brand

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